Organism : Pseudomonas aeruginosa | Module List :
PA0524 norB

nitric-oxide reductase subunit B (NCBI)

CircVis
Functional Annotations (9)
Function System
Nitric oxide reductase large subunit cog/ cog
iron ion binding go/ molecular_function
electron transport go/ biological_process
aerobic respiration go/ biological_process
integral to membrane go/ cellular_component
nitric oxide reductase activity go/ molecular_function
heme binding go/ molecular_function
Nitrogen metabolism kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0524
(Mouseover regulator name to see its description)

PA0524 is regulated by 13 influences and regulates 0 modules.
Regulators for PA0524 norB (13)
Regulator Module Operator
PA0512 110 tf
PA0513 110 tf
PA0514 110 tf
PA0515 110 tf
PA2577 110 tf
PA3391 110 tf
PA3689 110 tf
PA3815 110 tf
PA3932 110 tf
PA0512 458 tf
PA0513 458 tf
PA0514 458 tf
PA3391 458 tf

Warning: PA0524 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3052 1.10e-07 TCTTGAtTgCgaTCAAGCa
Loader icon
3053 2.00e-01 aCctCCtGGAATgC.TcGaGcGaA
Loader icon
3732 1.90e-06 aaTCTTGAtTcccaTCAAGCaaG
Loader icon
3733 1.90e+00 CcccgCtTcCTAtgATC
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0524

PA0524 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Nitric oxide reductase large subunit cog/ cog
iron ion binding go/ molecular_function
electron transport go/ biological_process
aerobic respiration go/ biological_process
integral to membrane go/ cellular_component
nitric oxide reductase activity go/ molecular_function
heme binding go/ molecular_function
Nitrogen metabolism kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for PA0524

PA0524 has total of 18 gene neighbors in modules 110, 458
Gene neighbors (18)
Gene Common Name Description Module membership
PA0509 nirN probable c-type cytochrome (NCBI) 458, 529
PA0511 nirJ heme d1 biosynthesis protein NirJ (NCBI) 456, 458
PA0512 PA0512 hypothetical protein (NCBI) 458, 529
PA0519 nirS nitrite reductase precursor (NCBI) 110, 456
PA0520 nirQ regulatory protein NirQ (NCBI) 110, 458
PA0521 PA0521 probable cytochrome c oxidase subunit (NCBI) 110, 458
PA0522 PA0522 hypothetical protein (NCBI) 110, 529
PA0523 norC nitric-oxide reductase subunit C (NCBI) 110, 458
PA0524 norB nitric-oxide reductase subunit B (NCBI) 110, 458
PA0525 PA0525 probable dinitrification protein NorD (NCBI) 110, 458
PA0526 PA0526 hypothetical protein (NCBI) 110, 529
PA2662 PA2662 hypothetical protein (NCBI) 74, 110
PA2663 PA2663 hypothetical protein (NCBI) 74, 110
PA2664 fhp flavohemoprotein (NCBI) 58, 110
PA3391 nosR regulatory protein NosR (NCBI) 110, 458
PA3392 nosZ nitrous-oxide reductase precurser (NCBI) 110, 458
PA3393 nosD NosD protein (NCBI) 458, 497
PA3394 nosF NosF protein (NCBI) 458, 497
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0524
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend