Organism : Pseudomonas aeruginosa | Module List :
PA1272 cobO

cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI)

CircVis
Functional Annotations (7)
Function System
ATP:corrinoid adenosyltransferase cog/ cog
ATP binding go/ molecular_function
cob(I)yrinic acid a,c-diamide adenosyltransferase activity go/ molecular_function
cobalamin biosynthetic process go/ biological_process
Porphyrin and chlorophyll metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
cobA tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1272
(Mouseover regulator name to see its description)

PA1272 is regulated by 44 influences and regulates 0 modules.
Regulators for PA1272 cobO (44)
Regulator Module Operator
PA0179 277 tf
PA0564 277 tf
PA0707 277 tf
PA0763 277 tf
PA0784 277 tf
PA0890 277 tf
PA0893 277 tf
PA1335 277 tf
PA1945 277 tf
PA3285 277 tf
PA3563 277 tf
PA3571 277 tf
PA4269 277 tf
PA4703 277 tf
PA4755 277 tf
PA5105 277 tf
PA5342 277 tf
PA5403 277 tf
PA5511 277 tf
PA5550 277 tf
PA5562 277 tf
PA0564 83 tf
PA0784 83 tf
PA0890 83 tf
PA0893 83 tf
PA1335 83 tf
PA1754 83 tf
PA1945 83 tf
PA2246 83 tf
PA2921 83 tf
PA3002 83 tf
PA3477 83 tf
PA3563 83 tf
PA4269 83 tf
PA4703 83 tf
PA4769 83 tf
PA5293 83 tf
PA5342 83 tf
PA5365 83 tf
PA5431 83 tf
PA5506 83 tf
PA5511 83 tf
PA5550 83 tf
PA5562 83 tf

Warning: PA1272 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2998 3.20e-04 GcgAGCCaGGAGACCtGCCt
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2999 6.30e+03 CATACA
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3382 7.90e+01 GcGAGCCaGGAGACCtGCC
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3383 2.80e+03 TATTTT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1272

PA1272 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
ATP:corrinoid adenosyltransferase cog/ cog
ATP binding go/ molecular_function
cob(I)yrinic acid a,c-diamide adenosyltransferase activity go/ molecular_function
cobalamin biosynthetic process go/ biological_process
Porphyrin and chlorophyll metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
cobA tigr/ tigrfam
Module neighborhood information for PA1272

PA1272 has total of 26 gene neighbors in modules 83, 277
Gene neighbors (26)
Gene Common Name Description Module membership
PA1271 PA1271 probable tonB-dependent receptor (NCBI) 83, 520
PA1272 cobO cob(I)yrinic acid a,c-diamide adenosyltransferase (NCBI) 83, 277
PA1273 cobB cobyrinic acid a,c-diamide synthase (NCBI) 83, 277
PA1274 PA1274 hypothetical protein (NCBI) 83, 277
PA1275 cobD adenosylcobinamide-phosphate synthase (NCBI) 83, 277
PA1276 cobC hypothetical protein (NCBI) 83, 277
PA1277 cobQ cobyric acid synthase (NCBI) 83, 277
PA1278 cobP adenosylcobinamide kinase (NCBI) 83, 277
PA1279 cobU nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI) 83, 277
PA1280 PA1280 hypothetical protein (NCBI) 83, 277
PA1281 cobV cobalamin synthase (NCBI) 83, 277
PA2903 cobJ precorrin-3 methylase CobJ (NCBI) 83, 277
PA2904 cobI precorrin-2 methyltransferase (NCBI) 83, 277
PA2905 cobH precorrin-8X methylmutase (NCBI) 83, 277
PA2906 PA2906 probable oxidoreductase (NCBI) 83, 440
PA2911 PA2911 probable TonB-dependent receptor (NCBI) 83, 408
PA2912 PA2912 probable ATP-binding component of ABC transporter (NCBI) 83, 408
PA2913 PA2913 hypothetical protein (NCBI) 23, 83
PA2914 PA2914 probable permease of ABC transporter (NCBI) 83, 408
PA2944 cobN cobalamin biosynthetic protein CobN (NCBI) 60, 83
PA2945 PA2945 hypothetical protein (NCBI) 83, 277
PA3642 rnhB ribonuclease HII (NCBI) 131, 277
PA3643 lpxB lipid-A-disaccharide synthase (NCBI) 270, 277
PA3820 secF protein export protein SecF (NCBI) 270, 277
PA3821 secD protein export protein SecD (NCBI) 277, 453
PA4237 rplQ 50S ribosomal protein L17 (NCBI) 277, 329
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1272
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend