Organism : Pseudomonas aeruginosa | Module List :
PA2399 pvdD

pyoverdine synthetase D (NCBI)

CircVis
Functional Annotations (7)
Function System
Non-ribosomal peptide synthetase modules and related proteins cog/ cog
biosynthetic process go/ biological_process
hydrolase activity, acting on ester bonds go/ molecular_function
ligase activity go/ molecular_function
phosphopantetheine binding go/ molecular_function
cofactor binding go/ molecular_function
AA-adenyl-dom tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2399
(Mouseover regulator name to see its description)

PA2399 is regulated by 72 influences and regulates 0 modules.
Regulators for PA2399 pvdD (72)
Regulator Module Operator
PA0159 369 tf
PA0479 369 tf
PA0535 369 tf
PA0707 369 tf
PA1226 369 tf
PA1484 369 tf
PA1539 369 tf
PA1570 369 tf
PA1599 369 tf
PA1884 369 tf
PA2028 369 tf
PA2076 369 tf
PA2273 369 tf
PA2337 369 tf
PA2383 369 tf
PA2387 369 tf
PA2417 369 tf
PA2426 369 tf
PA2497 369 tf
PA2519 369 tf
PA2848 369 tf
PA2917 369 tf
PA3410 369 tf
PA3604 369 tf
PA3804 369 tf
PA3815 369 tf
PA4132 369 tf
PA4145 369 tf
PA4196 369 tf
PA4279 369 tf
PA4778 369 tf
PA4784 369 tf
PA4806 369 tf
PA5274 369 tf
PA5356 369 tf
PA5438 369 tf
PA0159 379 tf
PA0479 379 tf
PA0535 379 tf
PA0707 379 tf
PA1226 379 tf
PA1484 379 tf
PA1539 379 tf
PA1570 379 tf
PA1599 379 tf
PA1884 379 tf
PA2028 379 tf
PA2076 379 tf
PA2273 379 tf
PA2337 379 tf
PA2383 379 tf
PA2387 379 tf
PA2417 379 tf
PA2426 379 tf
PA2497 379 tf
PA2519 379 tf
PA2917 379 tf
PA3410 379 tf
PA3604 379 tf
PA3804 379 tf
PA3815 379 tf
PA4132 379 tf
PA4145 379 tf
PA4196 379 tf
PA4279 379 tf
PA4778 379 tf
PA4784 379 tf
PA4806 379 tf
PA5274 379 tf
PA5301 379 tf
PA5356 379 tf
PA5438 379 tf

Warning: PA2399 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3562 2.30e-04 GAgGccagagcgggaAAATTtA
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3563 8.40e+00 GccGTtcCAcaGGcaAcccGacAa
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3582 1.00e-04 GAgGccagagcgggaAAATTtA
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3583 9.30e+00 gaatctCcaGtTGcCtgTGgAAc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2399

PA2399 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Non-ribosomal peptide synthetase modules and related proteins cog/ cog
biosynthetic process go/ biological_process
hydrolase activity, acting on ester bonds go/ molecular_function
ligase activity go/ molecular_function
phosphopantetheine binding go/ molecular_function
cofactor binding go/ molecular_function
AA-adenyl-dom tigr/ tigrfam
Module neighborhood information for PA2399

PA2399 has total of 15 gene neighbors in modules 369, 379
Gene neighbors (15)
Gene Common Name Description Module membership
PA2386 pvdA L-ornithine N5-oxygenase (NCBI) 369, 379
PA2389 PA2389 hypothetical protein (NCBI) 369, 379
PA2392 pvdP PvdP (NCBI) 369, 379
PA2393 PA2393 probable dipeptidase precursor (NCBI) 369, 379
PA2394 pvdN PvdN (NCBI) 369, 379
PA2395 pvdO PvdO (NCBI) 369, 379
PA2396 pvdF pyoverdine synthetase F (NCBI) 369, 379
PA2397 pvdE pyoverdine biosynthesis protein PvdE (NCBI) 369, 379
PA2399 pvdD pyoverdine synthetase D (NCBI) 369, 379
PA2400 pvdJ PvdJ (NCBI) 369, 379
PA2402 PA2402 probable non-ribosomal peptide synthetase (NCBI) 369, 379
PA2411 PA2411 probable thioesterase (NCBI) 369, 379
PA2412 PA2412 hypothetical protein (NCBI) 369, 379
PA2413 pvdH diaminobutyrate--2-oxoglutarate aminotransferase (NCBI) 369, 379
PA2424 pvdL PvdL (NCBI) 115, 369
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2399
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend