Organism : Pseudomonas aeruginosa | Module List :
PA2524

probable two-component sensor (NCBI)

CircVis
Functional Annotations (9)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
ATP binding go/ molecular_function
sensory perception go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
cztS_silS_copS tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2524
(Mouseover regulator name to see its description)

PA2524 is regulated by 19 influences and regulates 0 modules.
Regulators for PA2524 (19)
Regulator Module Operator
PA0218 418 tf
PA0527 418 tf
PA0828 418 tf
PA1980 418 tf
PA2354 418 tf
PA3711 418 tf
PA4269 418 tf
PA0167 226 tf
PA0873 226 tf
PA1015 226 tf
PA1201 226 tf
PA2758 226 tf
PA2885 226 tf
PA3782 226 tf
PA4070 226 tf
PA4269 226 tf
PA4703 226 tf
PA4806 226 tf
PA5380 226 tf

Warning: PA2524 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3280 2.00e+04 TTCaggCcGggTTCAGC.GA
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3281 9.70e+03 AGAAAT
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3658 5.80e-04 TaaCgg.at.gTaAt
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3659 6.90e-03 CGcTGGC.A.gcTGcCGgCgA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2524

PA2524 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
ATP binding go/ molecular_function
sensory perception go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
cztS_silS_copS tigr/ tigrfam
Module neighborhood information for PA2524

PA2524 has total of 36 gene neighbors in modules 226, 418
Gene neighbors (36)
Gene Common Name Description Module membership
PA0100 PA0100 hypothetical protein (NCBI) 11, 226
PA1243 PA1243 probable sensor/response regulator hybrid (NCBI) 189, 226
PA1331 PA1331 hypothetical protein (NCBI) 187, 226
PA1343 PA1343 hypothetical protein (NCBI) 202, 226
PA1435 PA1435 probable Resistance-Nodulation-Cell Division (RND) efflux membrane fusion protein precursor (NCBI) 418, 538
PA1436 PA1436 probable Resistance-Nodulation-Cell Division (RND) efflux transporter (NCBI) 418, 538
PA1437 PA1437 probable two-component response regulator (NCBI) 418, 538
PA1438 PA1438 probable two-component sensor (NCBI) 418, 538
PA1970 PA1970 hypothetical protein (NCBI) 108, 226
PA2119 PA2119 alcohol dehydrogenase (Zn-dependent) (NCBI) 26, 226
PA2372 PA2372 hypothetical protein (NCBI) 200, 226
PA2476 dsbG thiol:disulfide interchange protein DsbG (NCBI) 290, 418
PA2477 PA2477 probable thiol:disulfide interchange protein (NCBI) 290, 418
PA2478 PA2478 probable thiol:disulfide interchange protein (NCBI) 290, 418
PA2479 PA2479 probable two-component response regulator (NCBI) 290, 418
PA2480 PA2480 probable two-component sensor (NCBI) 290, 418
PA2506 PA2506 hypothetical protein (NCBI) 79, 418
PA2522 czcC outer membrane protein precursor CzcC (NCBI) 418, 446
PA2523 PA2523 probable two-component response regulator (NCBI) 196, 418
PA2524 PA2524 probable two-component sensor (NCBI) 226, 418
PA2526 PA2526 probable Resistance-Nodulation-Cell Division (RND) efflux transporter (NCBI) 196, 418
PA2527 PA2527 probable Resistance-Nodulation-Cell Division (RND) efflux transporter (NCBI) 196, 418
PA2593 PA2593 hypothetical protein (NCBI) 226, 291
PA2724 PA2724 hypothetical protein (NCBI) 33, 226
PA2933 PA2933 probable major facilitator superfamily (MFS) transporter (NCBI) 246, 418
PA3221 csaA CsaA protein (NCBI) 189, 226
PA3412 PA3412 hypothetical protein (NCBI) 418, 500
PA3782 PA3782 probable transcriptional regulator (NCBI) 226, 245
PA3920 PA3920 probable metal transporting P-type ATPase (NCBI) 226, 407
PA4136 PA4136 probable major facilitator superfamily (MFS) transporter (NCBI) 61, 226
PA4290 PA4290 probable chemotaxis transducer (NCBI) 187, 226
PA4638 PA4638 hypothetical protein (NCBI) 31, 226
PA4803 PA4803 hypothetical protein (NCBI) 79, 418
PA4884 PA4884 hypothetical protein (NCBI) 418, 439
PA4885 irlR two-component response regulator (NCBI) 283, 418
PA4886 PA4886 probable two-component sensor (NCBI) 418, 434
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2524
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend