Organism : Pseudomonas aeruginosa | Module List :
PA3337 rfaD

ADP-L-glycero-D-mannoheptose 6-epimerase (NCBI)

CircVis
Functional Annotations (8)
Function System
Nucleoside-diphosphate-sugar epimerases cog/ cog
carbohydrate metabolic process go/ biological_process
ADP-glyceromanno-heptose 6-epimerase activity go/ molecular_function
cellular metabolic process go/ biological_process
NADP binding go/ molecular_function
Lipopolysaccharide biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
heptose_epim tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA3337
(Mouseover regulator name to see its description)

PA3337 is regulated by 34 influences and regulates 0 modules.
Regulators for PA3337 rfaD (34)
Regulator Module Operator
PA0515 21 tf
PA0527 21 tf
PA1050 21 tf
PA1196 21 tf
PA1290 21 tf
PA1490 21 tf
PA1949 21 tf
PA2126 21 tf
PA2484 21 tf
PA3006 21 tf
PA3458 21 tf
PA3757 21 tf
PA3815 21 tf
PA3879 21 tf
PA3948 21 tf
PA4132 21 tf
PA4493 21 tf
PA4853 21 tf
PA5105 21 tf
PA5253 21 tf
PA5356 21 tf
PA5428 21 tf
PA0448 429 tf
PA0515 429 tf
PA0527 429 tf
PA1196 429 tf
PA1290 429 tf
PA1544 429 tf
PA2126 429 tf
PA3006 429 tf
PA3458 429 tf
PA3879 429 tf
PA4132 429 tf
PA4853 429 tf

Warning: PA3337 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2878 1.00e-12 TTGatccAcgTCAac
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2879 3.70e+03 tgCTTccaACTgaAA
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3678 6.40e-14 TTGATcCAcgTCAa
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3679 1.00e+03 AAT.tcctAT.AATtcaTt
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA3337

PA3337 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Nucleoside-diphosphate-sugar epimerases cog/ cog
carbohydrate metabolic process go/ biological_process
ADP-glyceromanno-heptose 6-epimerase activity go/ molecular_function
cellular metabolic process go/ biological_process
NADP binding go/ molecular_function
Lipopolysaccharide biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
heptose_epim tigr/ tigrfam
Module neighborhood information for PA3337

PA3337 has total of 19 gene neighbors in modules 21, 429
Gene neighbors (19)
Gene Common Name Description Module membership
PA0141 PA0141 hypothetical protein (NCBI) 429, 448
PA0200 PA0200 hypothetical protein (NCBI) 162, 429
PA1429 PA1429 probable cation-transporting P-type ATPase (NCBI) 21, 162
PA1546 hemN coproporphyrinogen III oxidase (NCBI) 429, 448
PA1673 PA1673 hypothetical protein (NCBI) 429, 448
PA1746 PA1746 hypothetical protein (NCBI) 21, 416
PA2753 PA2753 hypothetical protein (NCBI) 21, 429
PA3309 PA3309 hypothetical protein (NCBI) 21, 416
PA3337 rfaD ADP-L-glycero-D-mannoheptose 6-epimerase (NCBI) 21, 429
PA3880 PA3880 hypothetical protein (NCBI) 429, 448
PA4348 PA4348 hypothetical protein (NCBI) 429, 448
PA4352 PA4352 hypothetical protein (NCBI) 21, 429
PA4577 PA4577 hypothetical protein (NCBI) 21, 416
PA4587 ccpR cytochrome c551 peroxidase precursor (NCBI) 429, 448
PA5027 PA5027 hypothetical protein (NCBI) 21, 429
PA5170 arcD arginine/ornithine antiporter (NCBI) 21, 514
PA5232 PA5232 hypothetical protein (NCBI) 21, 38
PA5427 adhA alcohol dehydrogenase (NCBI) 21, 429
PA5475 PA5475 hypothetical protein (NCBI) 21, 429
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA3337
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend