Organism : Pseudomonas aeruginosa | Module List :
PA3706 wspC

probable protein methyltransferase (NCBI)

CircVis
Functional Annotations (4)
Function System
Methylase of chemotaxis methyl-accepting proteins cog/ cog
binding go/ molecular_function
protein-glutamate O-methyltransferase activity go/ molecular_function
Two-component system kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA3706
(Mouseover regulator name to see its description)

PA3706 is regulated by 28 influences and regulates 0 modules.
Regulators for PA3706 wspC (28)
Regulator Module Operator
PA0393 60 tf
PA0890 60 tf
PA0893 60 tf
PA1335 60 tf
PA2622 60 tf
PA2859 60 tf
PA3002 60 tf
PA3804 60 tf
PA4547 60 tf
PA5166 60 tf
PA5562 60 tf
PA0034 388 tf
PA0159 388 tf
PA0179 388 tf
PA0893 388 tf
PA1097 388 tf
PA1430 388 tf
PA1760 388 tf
PA1998 388 tf
PA2047 388 tf
PA2359 388 tf
PA2586 388 tf
PA2588 388 tf
PA4296 388 tf
PA4547 388 tf
PA4778 388 tf
PA5059 388 tf
PA5105 388 tf

Warning: PA3706 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2952 2.30e+04 AGaaTcGACAaATTGTTaA
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2953 6.90e+04 ACtgGaAaTCC
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3600 1.60e+00 agTcttgGaaAAagA
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3601 1.00e+04 TTtgccGgcaTTcTa
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA3706

PA3706 is enriched for 4 functions in 3 categories.
Enrichment Table (4)
Function System
Methylase of chemotaxis methyl-accepting proteins cog/ cog
binding go/ molecular_function
protein-glutamate O-methyltransferase activity go/ molecular_function
Two-component system kegg/ kegg pathway
Module neighborhood information for PA3706

PA3706 has total of 54 gene neighbors in modules 60, 388
Gene neighbors (54)
Gene Common Name Description Module membership
PA0156 PA0156 probable Resistance-Nodulation-Cell Division (RND) efflux membrane fusion protein precursor (NCBI) 127, 388
PA0157 PA0157 probable Resistance-Nodulation-Cell Division (RND) efflux membrane fusion protein precursor (NCBI) 127, 388
PA0158 PA0158 probable Resistance-Nodulation-Cell Division (RND) efflux transporter (NCBI) 127, 388
PA0395 pilT twitching motility protein PilT (NCBI) 127, 388
PA0454 PA0454 hypothetical protein (NCBI) 60, 268
PA0503 PA0503 probable biotin synthesis protein BioC (NCBI) 60, 91
PA0569 PA0569 hypothetical protein (NCBI) 60, 493
PA0704 PA0704 amidase (NCBI) 388, 443
PA0799 PA0799 probable helicase (NCBI) 20, 60
PA0893 argR transcriptional regulator ArgR (NCBI) 60, 160
PA0928 gacS sensor/response regulator hybrid (NCBI) 81, 388
PA1054 PA1054 probable NADH dehydrogenase (NCBI) 60, 82
PA1055 PA1055 hypothetical protein (NCBI) 60, 82
PA1056 PA1056 NADH dehydrogenase subunit N (NCBI) 60, 82
PA1057 PA1057 hypothetical protein (NCBI) 60, 82
PA1058 PA1058 hypothetical protein (NCBI) 60, 82
PA1059 PA1059 hypothetical protein (NCBI) 60, 82
PA1105 fliJ flagellar protein (NCBI) 60, 493
PA1119 PA1119 probable outer membrane protein precursor (NCBI) 388, 390
PA1120 PA1120 hypothetical protein (NCBI) 20, 388
PA1121 PA1121 hypothetical protein (NCBI) 159, 388
PA1967 PA1967 hypothetical protein (NCBI) 166, 388
PA2049 PA2049 hypothetical protein (NCBI) 60, 320
PA2617 aat leucyl/phenylalanyl-tRNA--protein transferase (NCBI) 60, 390
PA2618 PA2618 arginyl-tRNA-protein transferase (NCBI) 388, 390
PA2858 PA2858 hypothetical protein (NCBI) 60, 117
PA2944 cobN cobalamin biosynthetic protein CobN (NCBI) 60, 83
PA3070 PA3070 hypothetical protein (NCBI) 60, 94
PA3073 PA3073 hypothetical protein (NCBI) 60, 387
PA3091 PA3091 hypothetical protein (NCBI) 127, 388
PA3211 PA3211 probable permease of ABC transporter (NCBI) 60, 338
PA3340 PA3340 hypothetical protein (NCBI) 388, 437
PA3465 PA3465 hypothetical protein (NCBI) 38, 388
PA3702 wspR probable two-component response regulator (NCBI) 81, 388
PA3703 wspF probable methylesterase (NCBI) 53, 388
PA3704 wspE probable chemotaxis sensor/effector fusion protein (NCBI) 81, 388
PA3705 wspD hypothetical protein (NCBI) 81, 388
PA3706 wspC probable protein methyltransferase (NCBI) 60, 388
PA3707 wspB hypothetical protein (NCBI) 3, 388
PA3708 wspA probable chemotaxis transducer (NCBI) 81, 388
PA3864 PA3864 hypothetical protein (NCBI) 60, 268
PA4117 PA4117 probable bacteriophytochrome (NCBI) 35, 388
PA4145 PA4145 probable transcriptional regulator (NCBI) 60, 511
PA4285 recC exodeoxyribonuclease V gamma chain (NCBI) 49, 60
PA4312 PA4312 hypothetical protein (NCBI) 388, 492
PA4313 PA4313 hypothetical protein (NCBI) 31, 388
PA4601 morA motility regulator (NCBI) 60, 174
PA4617 PA4617 hypothetical protein (NCBI) 14, 60
PA4646 upp uracil phosphoribosyltransferase (NCBI) 60, 499
PA4647 uraA uracil permease (NCBI) 60, 499
PA4677 PA4677 hypothetical protein (NCBI) 127, 388
PA5022 PA5022 hypothetical protein (NCBI) 60, 253
PA5112 estA esterase EstA (NCBI) 193, 388
PA5567 PA5567 tRNA modification GTPase (NCBI) 60, 528
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA3706
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend