Organism : Pseudomonas aeruginosa | Module List :
PA3976 thiE

thiamine-phosphate diphosphorylase (NCBI)

CircVis
Functional Annotations (6)
Function System
Thiamine monophosphate synthase cog/ cog
thiamine-phosphate diphosphorylase activity go/ molecular_function
thiamine biosynthetic process go/ biological_process
Thiamine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
thiE tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA3976
(Mouseover regulator name to see its description)

PA3976 is regulated by 38 influences and regulates 0 modules.
Regulators for PA3976 thiE (38)
Regulator Module Operator
PA3604 286 tf
PA3804 286 tf
PA3948 286 tf
PA4057 286 tf
PA4238 286 tf
PA4275 286 tf
PA4451 286 tf
PA4745 286 tf
PA4853 286 tf
PA5337 286 tf
PA5344 286 tf
PA0167 106 tf
PA0393 106 tf
PA0701 106 tf
PA0815 106 tf
PA1397 106 tf
PA1539 106 tf
PA1898 106 tf
PA2196 106 tf
PA2622 106 tf
PA2665 106 tf
PA2696 106 tf
PA2899 106 tf
PA2957 106 tf
PA3002 106 tf
PA3126 106 tf
PA3184 106 tf
PA3225 106 tf
PA3604 106 tf
PA4052 106 tf
PA4269 106 tf
PA4270 106 tf
PA4275 106 tf
PA4451 106 tf
PA4745 106 tf
PA4890 106 tf
PA5166 106 tf
PA5324 106 tf

Warning: PA3976 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3044 3.10e-01 GaTcgTTcAcaaaAcTttGC
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3045 1.50e+00 tgTAG.AtacgCgcc
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3400 1.80e+00 TATaGTcCtgatAat
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3401 1.30e-01 CtCGGtGcCGGaGAGcCGGCGA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA3976

PA3976 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Thiamine monophosphate synthase cog/ cog
thiamine-phosphate diphosphorylase activity go/ molecular_function
thiamine biosynthetic process go/ biological_process
Thiamine metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
thiE tigr/ tigrfam
Module neighborhood information for PA3976

PA3976 has total of 29 gene neighbors in modules 106, 286
Gene neighbors (29)
Gene Common Name Description Module membership
PA0009 glyQ glycyl-tRNA synthetase alpha subunit (NCBI) 106, 453
PA0316 serA D-3-phosphoglycerate dehydrogenase (NCBI) 106, 433
PA0937 PA0937 hypothetical protein (NCBI) 281, 286
PA1757 thrH homoserine kinase (NCBI) 249, 286
PA1796 folD 5,10-methylene-tetrahydrofolate dehydrogenase / cyclohydrolase (NCBI) 106, 544
PA2629 purB adenylosuccinate lyase (NCBI) 106, 544
PA3654 pyrH uridylate kinase (NCBI) 106, 453
PA3686 adk adenylate kinase (NCBI) 123, 286
PA3763 purL phosphoribosylformylglycinamidine synthase (NCBI) 106, 204
PA3770 guaB inositol-5-monophosphate dehydrogenase (NCBI) 90, 106
PA3975 thiD phosphomethylpyrimidine kinase (NCBI) 86, 286
PA3976 thiE thiamine-phosphate diphosphorylase (NCBI) 106, 286
PA3987 leuS leucyl-tRNA synthetase (NCBI) 286, 398
PA4560 ileS isoleucyl-tRNA synthetase (NCBI) 43, 106
PA4602 glyA3 serine hydroxymethyltransferase (NCBI) 106, 433
PA4722 PA4722 hypothetical protein (NCBI) 286, 384
PA4723 dksA suppressor protein DksA (NCBI) 122, 286
PA4724 PA4724 glutamyl-tRNA synthetase (NCBI) 286, 501
PA4758 carA carbamoyl-phosphate synthase small subunit (NCBI) 106, 453
PA4888 PA4888 hypothetical protein (NCBI) 106, 332
PA4889 PA4889 probable oxidoreductase (NCBI) 106, 332
PA4890 PA4890 hypothetical protein (NCBI) 106, 332
PA4953 motB flagellar motor protein (NCBI) 8, 286
PA4954 motA flagellar motor protein (NCBI) 8, 286
PA4956 rhdA thiosulfate sulfurtransferase (NCBI) 286, 433
PA4957 psd phosphatidylserine decarboxylase (NCBI) 286, 433
PA5296 rep ATP-dependent DNA helicase Rep (NCBI) 106, 230
PA5414 PA5414 hypothetical protein (NCBI) 281, 286
PA5462 PA5462 hypothetical protein (NCBI) 276, 286
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA3976
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend