Organism : Pseudomonas aeruginosa | Module List :
PA4367

hypothetical protein (NCBI)

CircVis
Functional Annotations (5)
Function System
Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain cog/ cog
cyclic nucleotide biosynthetic process go/ biological_process
phosphorus-oxygen lyase activity go/ molecular_function
intracellular signal transduction go/ biological_process
GGDEF tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4367
(Mouseover regulator name to see its description)

PA4367 is regulated by 43 influences and regulates 0 modules.
Regulators for PA4367 (43)
Regulator Module Operator
PA0125 378 tf
PA0163 378 tf
PA0167 378 tf
PA0289 378 tf
PA0763 378 tf
PA1335 378 tf
PA1619 378 tf
PA1754 378 tf
PA1760 378 tf
PA2047 378 tf
PA2312 378 tf
PA2737 378 tf
PA3034 378 tf
PA3045 378 tf
PA3622 378 tf
PA3804 378 tf
PA3895 378 tf
PA3921 378 tf
PA3995 378 tf
PA4269 378 tf
PA4462 378 tf
PA4581 378 tf
PA5166 378 tf
PA5253 378 tf
PA5483 378 tf
PA0279 464 tf
PA0393 464 tf
PA0436 464 tf
PA0707 464 tf
PA0893 464 tf
PA0905 464 tf
PA1484 464 tf
PA1754 464 tf
PA2586 464 tf
PA3002 464 tf
PA3563 464 tf
PA3721 464 tf
PA3879 464 tf
PA4270 464 tf
PA4547 464 tf
PA5125 464 tf
PA5166 464 tf
PA5324 464 tf

Warning: PA4367 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3580 5.50e+02 caGtcTtTTtC
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3581 3.80e+03 AAg.cgCTATCAt
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3744 2.00e+01 TtTtcgTt..aA
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3745 1.20e+02 TaGGtA.AgCT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4367

PA4367 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Predicted signal transduction protein containing a membrane domain, an EAL and a GGDEF domain cog/ cog
cyclic nucleotide biosynthetic process go/ biological_process
phosphorus-oxygen lyase activity go/ molecular_function
intracellular signal transduction go/ biological_process
GGDEF tigr/ tigrfam
Module neighborhood information for PA4367

PA4367 has total of 34 gene neighbors in modules 378, 464
Gene neighbors (34)
Gene Common Name Description Module membership
PA0387 PA0387 putative deoxyribonucleotide triphosphate pyrophosphatase (NCBI) 129, 464
PA0388 PA0388 hypothetical protein (NCBI) 129, 464
PA0467 PA0467 hypothetical protein (NCBI) 127, 378
PA0589 glpE thiosulfate sulfurtransferase (NCBI) 202, 464
PA0590 apaH diadenosinetetraphosphatase (NCBI) 18, 464
PA0591 PA0591 hypothetical protein (NCBI) 202, 464
PA1040 PA1040 hypothetical protein (NCBI) 338, 378
PA1307 PA1307 hypothetical protein (NCBI) 378, 513
PA1684 PA1684 probable oxidase (NCBI) 214, 378
PA1802 clpX ATP-dependent protease ATP-binding subunit (NCBI) 57, 464
PA1803 lon Lon protease (NCBI) 393, 464
PA2080 kynU kynureninase (NCBI) 179, 464
PA2530 PA2530 hypothetical protein (NCBI) 378, 540
PA2631 PA2631 probable acetyl transferase (NCBI) 129, 464
PA2632 PA2632 hypothetical protein (NCBI) 390, 464
PA2633 PA2633 hypothetical protein (NCBI) 390, 464
PA2710 PA2710 hypothetical protein (NCBI) 203, 378
PA3052 PA3052 hypothetical protein (NCBI) 10, 378
PA3053 PA3053 probable hydrolytic enzyme (NCBI) 7, 378
PA3214 PA3214 hypothetical protein (NCBI) 81, 378
PA3856 PA3856 hypothetical protein (NCBI) 378, 443
PA3944 PA3944 hypothetical protein (NCBI) 378, 443
PA3970 amn AMP nucleosidase (NCBI) 378, 523
PA4027 PA4027 hypothetical protein (NCBI) 185, 378
PA4115 PA4115 hypothetical protein (NCBI) 378, 523
PA4367 PA4367 hypothetical protein (NCBI) 378, 464
PA4947 amiB N-acetylmuramoyl-L-alanine amidase (NCBI) 81, 464
PA5125 ntrC two-component response regulator NtrC (NCBI) 203, 464
PA5162 rmlD dTDP-4-dehydrorhamnose reductase (NCBI) 179, 464
PA5163 rmlA glucose-1-phosphate thymidylyltransferase (NCBI) 179, 464
PA5164 rmlC dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI) 179, 464
PA5487 PA5487 hypothetical protein (NCBI) 91, 378
PA5488 PA5488 hypothetical protein (NCBI) 378, 488
PA5521 PA5521 probable short-chain dehydrogenase (NCBI) 28, 378
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4367
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend