Organism : Pseudomonas aeruginosa | Module List :
PA4391

hypothetical protein (NCBI)

CircVis
Functional Annotations (2)
Function System
cytokine receptor activity go/ molecular_function
membrane go/ cellular_component
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4391
(Mouseover regulator name to see its description)

PA4391 is regulated by 28 influences and regulates 0 modules.
Regulators for PA4391 (28)
Regulator Module Operator
PA0268 48 tf
PA1159 48 tf
PA1380 48 tf
PA1539 48 tf
PA2047 48 tf
PA2921 48 tf
PA3133 48 tf
PA3622 48 tf
PA4169 48 tf
PA4493 48 tf
PA4853 48 tf
PA5365 48 tf
PA5403 48 tf
PA5437 48 tf
PA0125 299 tf
PA0236 299 tf
PA0961 299 tf
PA2047 299 tf
PA2115 299 tf
PA2921 299 tf
PA3133 299 tf
PA3322 299 tf
PA3604 299 tf
PA3921 299 tf
PA4853 299 tf
PA5253 299 tf
PA5403 299 tf
PA5437 299 tf

Warning: PA4391 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2928 1.30e-03 aatcTCtcgttcctTTgcCtG
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2929 1.20e-01 ccgaGatagGgaAAt.tCtG
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3426 4.10e+03 tagcCcTa.gaaAa
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3427 1.30e+01 AAggAAAa
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4391

PA4391 is enriched for 2 functions in 2 categories.
Enrichment Table (2)
Function System
cytokine receptor activity go/ molecular_function
membrane go/ cellular_component
Module neighborhood information for PA4391

PA4391 has total of 48 gene neighbors in modules 48, 299
Gene neighbors (48)
Gene Common Name Description Module membership
PA0236 PA0236 probable transcriptional regulator (NCBI) 299, 335
PA0310 PA0310 hypothetical protein (NCBI) 48, 422
PA0712 PA0712 hypothetical protein (NCBI) 48, 190
PA0923 dinP DNA polymerase IV (NCBI) 299, 412
PA1026 PA1026 hypothetical protein (NCBI) 48, 299
PA1361 PA1361 probable transporter (NCBI) 48, 377
PA1379 PA1379 short chain dehydrogenase (NCBI) 48, 372
PA1380 PA1380 probable transcriptional regulator (NCBI) 48, 372
PA1593 PA1593 hypothetical protein (NCBI) 247, 299
PA1594 PA1594 hypothetical protein (NCBI) 229, 299
PA1839 PA1839 hypothetical protein (NCBI) 299, 501
PA1959 bacA bacitracin resistance protein (NCBI) 299, 412
PA2039 PA2039 hypothetical protein (NCBI) 247, 299
PA2558 PA2558 probable transport protein (NCBI) 48, 245
PA2561 PA2561 probable chemotaxis transducer (NCBI) 48, 151
PA2749 endA DNA-specific endonuclease I (NCBI) 138, 299
PA2750 PA2750 hypothetical protein (NCBI) 247, 299
PA2770 PA2770 hypothetical protein (NCBI) 139, 299
PA2972 PA2972 Maf-like protein (NCBI) 1, 299
PA3016 PA3016 hypothetical protein (NCBI) 48, 375
PA3280 oprO Pyrophosphate-specific outer membrane porin OprO precursor (NCBI) 48, 302
PA3281 PA3281 hypothetical protein (NCBI) 48, 240
PA3282 PA3282 hypothetical protein (NCBI) 48, 240
PA3283 PA3283 hypothetical protein (NCBI) 48, 240
PA3284 PA3284 hypothetical protein (NCBI) 48, 240
PA3388 PA3388 hypothetical protein (NCBI) 299, 351
PA3573 PA3573 probable major facilitator superfamily (MFS) transporter (NCBI) 181, 299
PA3606 PA3606 hypothetical protein (NCBI) 48, 181
PA3725 recJ single-stranded-DNA-specific exonuclease RecJ (NCBI) 65, 299
PA3990 PA3990 hypothetical protein (NCBI) 255, 299
PA4169 PA4169 hypothetical protein (NCBI) 48, 236
PA4233 PA4233 probable major facilitator superfamily (MFS) transporter (NCBI) 299, 422
PA4280 birA biotin--protein ligase (NCBI) 299, 474
PA4391 PA4391 hypothetical protein (NCBI) 48, 299
PA4445 PA4445 hypothetical protein (NCBI) 299, 422
PA4629 PA4629 hypothetical protein (NCBI) 65, 299
PA4634 PA4634 hypothetical protein (NCBI) 299, 351
PA4801 PA4801 hypothetical protein (NCBI) 299, 490
PA5021 PA5021 potassium/proton antiporter (RefSeq) 299, 306
PA5072 PA5072 probable chemotaxis transducer (NCBI) 48, 61
PA5120 PA5120 hypothetical protein (NCBI) 48, 501
PA5194 PA5194 hypothetical protein (NCBI) 247, 299
PA5365 phoU phosphate uptake regulatory protein PhoU (NCBI) 48, 371
PA5366 pstB ATP-binding component of ABC phosphate transporter (NCBI) 48, 371
PA5367 pstA membrane protein component of ABC phosphate transporter (NCBI) 48, 371
PA5368 pstC membrane protein component of ABC phosphate transporter (NCBI) 48, 302
PA5369 PA5369 hypothetical protein (NCBI) 48, 371
PA5437 PA5437 probable transcriptional regulator (NCBI) 299, 412
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4391
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend