Organism : Pseudomonas aeruginosa | Module List :
PA5045 ponA

penicillin-binding protein 1A (NCBI)

CircVis
Functional Annotations (10)
Function System
Membrane carboxypeptidase/penicillin-binding protein cog/ cog
peptidase activity go/ molecular_function
penicillin binding go/ molecular_function
peptidoglycan biosynthetic process go/ biological_process
peptidoglycan-based cell wall go/ cellular_component
transferase activity, transferring pentosyl groups go/ molecular_function
response to antibiotic go/ biological_process
Peptidoglycan biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
PBP_1a_fam tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA5045
(Mouseover regulator name to see its description)

PA5045 is regulated by 32 influences and regulates 0 modules.
Regulators for PA5045 ponA (32)
Regulator Module Operator
PA0456 59 tf
PA0576 59 tf
PA0893 59 tf
PA1269 59 tf
PA1504 59 tf
PA3804 59 tf
PA4052 59 tf
PA4269 59 tf
PA4270 59 tf
PA4275 59 tf
PA4279 59 tf
PA4451 59 tf
PA4462 59 tf
PA4745 59 tf
PA4769 59 tf
PA4853 59 tf
PA4890 59 tf
PA5337 59 tf
PA5344 59 tf
PA0393 91 tf
PA0890 91 tf
PA0893 91 tf
PA1526 91 tf
PA3285 91 tf
PA3804 91 tf
PA4070 91 tf
PA4269 91 tf
PA4547 91 tf
PA4755 91 tf
PA5125 91 tf
PA5483 91 tf
PA5562 91 tf

Warning: PA5045 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2950 9.10e+02 GccgCGCTtGC
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2951 3.90e+03 cgCGGgtt..TtC.tcTCtct
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3014 5.90e-02 cTtctTc.TtCatcTT
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3015 2.00e+02 ActAaAttTG.TcA.taT.TGAaC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA5045

PA5045 is enriched for 10 functions in 3 categories.
Enrichment Table (10)
Function System
Membrane carboxypeptidase/penicillin-binding protein cog/ cog
peptidase activity go/ molecular_function
penicillin binding go/ molecular_function
peptidoglycan biosynthetic process go/ biological_process
peptidoglycan-based cell wall go/ cellular_component
transferase activity, transferring pentosyl groups go/ molecular_function
response to antibiotic go/ biological_process
Peptidoglycan biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
PBP_1a_fam tigr/ tigrfam
Module neighborhood information for PA5045

PA5045 has total of 40 gene neighbors in modules 59, 91
Gene neighbors (40)
Gene Common Name Description Module membership
PA0428 PA0428 probable ATP-dependent RNA helicase (NCBI) 82, 91
PA0500 bioB biotin synthase (NCBI) 91, 368
PA0501 bioF 8-amino-7-oxononanoate synthase (NCBI) 91, 253
PA0502 PA0502 probable biotin biosynthesis protein bioH (NCBI) 91, 253
PA0503 PA0503 probable biotin synthesis protein BioC (NCBI) 60, 91
PA0553 PA0553 hypothetical protein (NCBI) 91, 205
PA0554 PA0554 hypothetical protein (NCBI) 59, 195
PA0705 PA0705 probable glycosyl transferase (NCBI) 59, 263
PA0832 PA0832 hypothetical protein (NCBI) 31, 59
PA0965 ruvC Holliday junction resolvase (NCBI) 59, 69
PA1162 dapE succinyl-diaminopimelate desuccinylase (NCBI) 59, 281
PA1180 phoQ two-component sensor PhoQ (NCBI) 91, 202
PA1193 PA1193 hypothetical protein (NCBI) 59, 442
PA1269 PA1269 probable transcriptional regulator (NCBI) 59, 143
PA1295 PA1295 hypothetical protein (NCBI) 59, 544
PA1530 PA1530 hypothetical protein (NCBI) 91, 511
PA2987 PA2987 probable ATP-binding component of ABC transporter (NCBI) 91, 353
PA3047 PA3047 probable D-alanyl-D-alanine carboxypeptidase (NCBI) 91, 130
PA3849 ndpA nucleoid-associated protein NdpA (NCBI) 59, 263
PA4002 rodA rod shape-determining protein (NCBI) 59, 478
PA4049 PA4049 hypothetical protein (NCBI) 91, 202
PA4116 PA4116 hypothetical protein (NCBI) 59, 195
PA4118 PA4118 hypothetical protein (NCBI) 59, 268
PA4715 PA4715 hypothetical protein (NCBI) 91, 420
PA4769 PA4769 probable transcriptional regulator (NCBI) 59, 117
PA4856 retS regulator of exopolysaccharide and type III secretion (NCBI) 59, 552
PA4965 PA4965 hypothetical protein (NCBI) 59, 442
PA4968 PA4968 hypothetical protein (NCBI) 59, 442
PA4969 PA4969 hypothetical protein (NCBI) 59, 218
PA4970 PA4970 hypothetical protein (NCBI) 59, 368
PA5000 PA5000 probable glycosyl transferase (NCBI) 59, 296
PA5037 PA5037 hypothetical protein (NCBI) 91, 144
PA5045 ponA penicillin-binding protein 1A (NCBI) 59, 91
PA5077 mdoH glucosyltransferase MdoH (NCBI) 91, 552
PA5110 fbp fructose-1,6-bisphosphatase (NCBI) 59, 225
PA5111 gloA3 lactoylglutathione lyase (NCBI) 91, 328
PA5131 pgm phosphoglyceromutase (NCBI) 91, 186
PA5322 algC phosphomannomutase AlgC (NCBI) 91, 214
PA5323 argB acetylglutamate kinase (NCBI) 91, 214
PA5487 PA5487 hypothetical protein (NCBI) 91, 378
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA5045
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend