Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0900

FAD-dependent pyridine nucleotide-disulphide oxidoreductase (NCBI)

CircVis
Functional Annotations (7)
Function System
cytoplasm go/ cellular_component
electron transport go/ biological_process
oxidoreductase activity go/ molecular_function
cell redox homeostasis go/ biological_process
Fatty acid metabolism kegg/ kegg pathway
Phenylalanine metabolism kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0900
(Mouseover regulator name to see its description)

RSP_0900 is regulated by 20 influences and regulates 0 modules.
Regulators for RSP_0900 (20)
Regulator Module Operator
RSP_0794 129 tf
RSP_2165 129 tf
RSP_2494 129 tf
RSP_2572 129 tf
RSP_2606 129 tf
RSP_2888 129 tf
RSP_3341 129 tf
RSP_3680 129 tf
RSP_3731 129 tf
RSP_0327 142 tf
RSP_0755 142 tf
RSP_1034 142 tf
RSP_2346 142 tf
RSP_2606 142 tf
RSP_2838 142 tf
RSP_2850 142 tf
RSP_2888 142 tf
RSP_3179 142 tf
RSP_3665 142 tf
RSP_3731 142 tf

Warning: RSP_0900 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7978 2.00e+02 gtTaTA
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7979 3.20e+03 CaaaTTGtCgC
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8004 2.90e-02 aTAcGGTCGCaCCGGaCGcCCGGC
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8005 8.20e-06 aaCcC.Tc.cgtTgCGgaaaggat
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0900

RSP_0900 is enriched for 7 functions in 4 categories.
Enrichment Table (7)
Function System
cytoplasm go/ cellular_component
electron transport go/ biological_process
oxidoreductase activity go/ molecular_function
cell redox homeostasis go/ biological_process
Fatty acid metabolism kegg/ kegg pathway
Phenylalanine metabolism kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for RSP_0900

RSP_0900 has total of 25 gene neighbors in modules 129, 142
Gene neighbors (25)
Gene Common Name Description Module membership
RSP_0816 RSP_0816 Putative aminotransferase, class IV (NCBI) 129, 177
RSP_0817 RSP_0817 Putative anthranilate synthase component I and chorismate binding protein (NCBI) 129, 177
RSP_0824 pth Peptidyl-tRNA hydrolase (NCBI) 142, 323
RSP_0826 RSP_0826 hypothetical protein (NCBI) 4, 142
RSP_0899 RSP_0899 AhpC/TSA family protein (NCBI) 58, 142
RSP_0900 RSP_0900 FAD-dependent pyridine nucleotide-disulphide oxidoreductase (NCBI) 129, 142
RSP_0901 RSP_0901 N-6 Adenine-specific DNA methylase (NCBI) 142, 271
RSP_0902 RSP_0902 Hydrolase, haloacid dehalogenase-like hydrolase (NCBI) 142, 271
RSP_1101 RSP_1101 hypothetical protein (NCBI) 129, 356
RSP_1154 RSP_1154 hypothetical protein (NCBI) 129, 356
RSP_1155 RSP_1155 OstA-like protein (NCBI) 129, 356
RSP_3607 RSP_3607 hypothetical protein (NCBI) 129, 356
RSP_6024 RSP_6024 hypothetical protein (NCBI) 142, 243
RSP_6053 RSP_6053 putative phage tail-head adaptor (NCBI) 142, 266
RSP_6109 pufK Transcriptional regulatory protein pufK (NCBI) 142, 179
RSP_6114 RSP_6114 hypothetical protein (NCBI) 142, 321
RSP_6135 thi thiamine biosynthetic protein (NCBI) 142, 243
RSP_6175 RSP_6175 None 142, 241
RSP_6183 RSP_6183 hypothetical protein (NCBI) 40, 142
RSP_6186 RSP_6186 hypothetical protein (NCBI) 142, 205
RSP_6202 RSP_6202 hypothetical protein (NCBI) 142, 243
RSP_6212 RSP_6212 hypothetical protein (NCBI) 142, 167
RSP_6225 RSP_6225 hypothetical protein (NCBI) 142, 194
RSP_6230 RSP_6230 hypothetical protein (NCBI) 50, 142
RSP_6248 RSP_6248 putative class I holin (NCBI) 142, 165
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0900
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend