Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2557 asnB

putative asparagine synthetase (NCBI)

CircVis
Functional Annotations (8)
Function System
Asparagine synthase (glutamine-hydrolyzing) cog/ cog
asparagine synthase (glutamine-hydrolyzing) activity go/ molecular_function
asparagine biosynthetic process go/ biological_process
Alanine aspartate and glutamate metabolism kegg/ kegg pathway
Nitrogen metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2557
(Mouseover regulator name to see its description)

RSP_2557 is regulated by 15 influences and regulates 0 modules.
Regulators for RSP_2557 asnB (15)
Regulator Module Operator
RSP_0032 323 tf
RSP_1286 323 tf
RSP_1776 323 tf
RSP_1785 323 tf
RSP_1936 323 tf
RSP_2610 323 tf
RSP_2850 323 tf
RSP_3022 323 tf
RSP_3124 323 tf
RSP_3731 323 tf
RSP_0907 157 tf
RSP_1231 157 tf
RSP_1518 157 tf
RSP_3029 157 tf
RSP_3700 157 tf

Warning: RSP_2557 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8034 1.80e-04 Ata.aTCAgtgCa.ataatCtcAA
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8035 1.00e-01 CATGGTTccGCaatCGaaAGa
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8356 4.50e-08 aaaaAttttc.t.tGaTttTCa.t
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8357 2.50e+01 aaagtaaacGaTaTtgATaaT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2557

RSP_2557 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Asparagine synthase (glutamine-hydrolyzing) cog/ cog
asparagine synthase (glutamine-hydrolyzing) activity go/ molecular_function
asparagine biosynthetic process go/ biological_process
Alanine aspartate and glutamate metabolism kegg/ kegg pathway
Nitrogen metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for RSP_2557

RSP_2557 has total of 54 gene neighbors in modules 157, 323
Gene neighbors (54)
Gene Common Name Description Module membership
RSP_0008 RSP_0008 possible transporter, DME family, DMT superfamily (NCBI) 323, 340
RSP_0214 RSP_0214 possible uncharacterized phage protein (NCBI) 323, 325
RSP_0393 RSP_0393 probable riboflavin biosynthesis protein (NCBI) 107, 323
RSP_0563 RSP_0563 putative site-specific recombinase (NCBI) 220, 323
RSP_0581 RSP_0581 hypothetical protein (NCBI) 157, 163
RSP_0813 RSP_0813 Response regulator receiver protein (NCBI) 29, 157
RSP_0824 pth Peptidyl-tRNA hydrolase (NCBI) 142, 323
RSP_0953 RSP_0953 hypothetical protein (NCBI) 121, 323
RSP_1022 RSP_1022 conserved hypothetical protein (possibly transmembrane) (NCBI) 200, 323
RSP_1174 pip Prolyl aminopeptidase (NCBI) 35, 323
RSP_1213 RSP_1213 hypothetical protein (NCBI) 157, 293
RSP_1365 RSP_1365 hypothetical protein (NCBI) 182, 323
RSP_1384 RSP_1384 hypothetical protein (NCBI) 10, 323
RSP_1393 thiQ ABC thiamine transporter, ATPase subunit (NCBI) 31, 157
RSP_1432 RSP_1432 hypothetical protein (NCBI) 94, 157
RSP_1474 nspC putative carboxynorspermidine decarboxylase protein (NCBI) 271, 323
RSP_1527 RSP_1527 Helicase, UvrD/Rep family (NCBI) 110, 157
RSP_1780 RSP_1780 hypothetical protein (NCBI) 285, 323
RSP_1894 RSP_1894 hypothetical protein (NCBI) 323, 381
RSP_1924 RSP_1924 BioY family protein (NCBI) 296, 323
RSP_1993 RSP_1993 hypothetical protein (NCBI) 37, 323
RSP_2005 ybaU possible peptidyl-prolyl cis-trans isomerse (NCBI) 203, 323
RSP_2018 RSP_2018 hypothetical protein (NCBI) 308, 323
RSP_2215 RSP_2215 hypothetical protein (NCBI) 223, 323
RSP_2553 RSP_2553 hypothetical protein (NCBI) 141, 157
RSP_2555 RSP_2555 hypothetical protein (NCBI) 77, 157
RSP_2556 RSP_2556 hypothetical protein (NCBI) 77, 157
RSP_2557 asnB putative asparagine synthetase (NCBI) 157, 323
RSP_2558 RSP_2558 hypothetical protein (NCBI) 157, 316
RSP_2588 RSP_2588 hypothetical protein (NCBI) 157, 363
RSP_2746 RSP_2746 hypothetical protein (NCBI) 323, 335
RSP_2946 argC N-acetyl-gamma-glutamyl-phosphate reductase (NCBI) 299, 323
RSP_3022 RSP_3022 transcriptional regulator, TetR family (NCBI) 213, 323
RSP_3029 RSP_3029 transcriptional regulator, GntR family (NCBI) 97, 157
RSP_3031 RSP_3031 ABC peptide/opine transporter, inner membrane subunit (NCBI) 97, 157
RSP_3085 RSP_3085 Conserved hypothetical membrane protein (NCBI) 157, 176
RSP_3094 RSP_3094 Putative transmembrane anti-sigma factor (NCBI) 202, 323
RSP_3124 RSP_3124 transcriptional regulator, LysR family (NCBI) 1, 323
RSP_3125 RSP_3125 transcriptional regulator, ArsR familyy (NCBI) 1, 323
RSP_3253 RSP_3253 Predicted acetamidase / formamidase (NCBI) 157, 245
RSP_3255 RSP_3255 ABC peptide transporter, periplasmic binding protein (NCBI) 157, 245
RSP_3422 RSP_3422 hypothetical protein (NCBI) 251, 323
RSP_3592 aroA EPSP synthase, 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI) 203, 323
RSP_3655 RSP_3655 Dihydrodipicolinate synthase/N-acetylneuraminate lyase (NCBI) 105, 157
RSP_3675 RSP_3675 Esterase/lipase/thioesterase (NCBI) 44, 323
RSP_3677 RSP_3677 ABC sugar transporter, ATPase subunit (NCBI) 44, 323
RSP_3700 RSP_3700 transcriptional regulator, LacI family (NCBI) 157, 251
RSP_3701 RSP_3701 ABC sugar transporter, periplasmic binding protein (NCBI) 157, 251
RSP_3702 RSP_3702 ABC sugar transporter, ATPase subunit (NCBI) 157, 251
RSP_3703 RSP_3703 ABC sugar transporter, inner membrane subunit (NCBI) 157, 251
RSP_3704 RSP_3704 ribitol 2-dehydrogenase (NCBI) 157, 251
RSP_3705 RSP_3705 D-ribulokinase (NCBI) 157, 251
RSP_3797 RSP_3797 hypothetical protein (NCBI) 141, 157
RSP_3805 RSP_3805 possible integrase (NCBI) 157, 285
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2557
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend