Organism : Rhodobacter sphaeroides 2.4.1 | Module List:
Module 184 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 184

There are 12 regulatory influences for Module 184

Regulator Table (12)
Regulator Name Type
RSP_1231 tf
RSP_2922 tf
RSP_2888 tf
RSP_0958 tf
RSP_2410 tf
RSP_1163 tf
RSP_0927 tf
RSP_0774 tf
RSP_0728 tf
RSP_1925 tf
RSP_1890 tf
RSP_3684 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
8088 6.30e+00 ttA.aa.GGg.GccTCg.aaaGGA
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8089 4.70e-02 acTcTgct.CcGgCccTttt
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 184 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolism kegg category 4.11e-04 3.35e-03 14/30
Carbohydrate Metabolism kegg subcategory 6.97e-03 2.07e-02 5/30
Metabolism of Other Amino Acids kegg subcategory 1.47e-03 7.94e-03 3/30
Global kegg category 1.20e-05 2.05e-04 15/30
Metabolism kegg subcategory 1.20e-05 2.05e-04 15/30
Metabolic pathways kegg pathway 2.80e-05 4.29e-04 14/30
Microbial metabolism in diverse environments kegg pathway 2.33e-02 4.74e-02 4/30

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Metabolism cog category 2.24e-02 3.47e-02 15/30
Cell wall/membrane/envelope biogenesis cog subcategory 5.03e-03 8.38e-03 4/30
Coenzyme transport and metabolism cog subcategory 5.33e-03 8.85e-03 4/30
Secondary metabolites biosynthesis, transport and catabolism cog subcategory 5.44e-03 9.03e-03 3/30
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 184

There are 30 genes in Module 184

Gene Member Table (30)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
RSP_0661 RSP_0661 CDS None chromosome 1 2401243 2402133 - Probable tetrahydrofolate dehydrogenase/cyclohydrolase (NCBI) False
RSP_0662 RSP_0662 CDS None chromosome 1 2402130 2402447 - Putative chorismate mutase (NCBI) False
RSP_0774 RSP_0774 CDS None chromosome 1 2515853 2518174 - RNA binding protein (NCBI) True
RSP_0779 perM CDS None chromosome 1 2522512 2523639 + Lipocalin-related protein and Bos/Can/Equ allergen (NCBI) False
RSP_0848 xthA1 CDS None chromosome 1 2598593 2599381 + Exodeoxyribonuclease III (NCBI) False
RSP_1073 pgsA CDS None chromosome 1 2828256 2828921 - Phosphatidylglycerol phosphate synthase (NCBI) False
RSP_1096 RSP_1096 CDS None chromosome 1 2853937 2855244 - putative zinc protease (NCBI) False
RSP_1100 purH CDS None chromosome 1 2857807 2859396 - AICARFT/IMPCHase bienzyme (NCBI) False
RSP_1160 kdsB CDS None chromosome 1 2922660 2923457 - CMP-2-keto-3-deoxyoctulaosonic acid synthetase (NCBI) False
RSP_1461 kdtA CDS None chromosome 1 46812 47999 + Putative 3-deoxy-D-manno-octulosonic-acid transferase (NCBI) False
RSP_1671 RSP_1671 CDS None chromosome 1 262693 263349 + hypothetical protein (NCBI) False
RSP_1767 RSP_1767 CDS None chromosome 1 350457 351194 + possible N-formylglutamate amidohydrolase (NCBI) False
RSP_1815 gshB CDS None chromosome 1 403146 404096 + glutathione synthetase (NCBI) False
RSP_1862 RSP_1862 CDS None chromosome 1 460538 461575 + Dihydropteroate synthase, DHPS (NCBI) False
RSP_1897 mgtE CDS None chromosome 1 495484 496818 + Mg/Co/Ni transporter, MgtE (NCBI) False
RSP_2091 RSP_2091 CDS None chromosome 1 690398 691543 - Putative benzoate transporter, BenE (NCBI) False
RSP_2147 cysK CDS None chromosome 1 749066 750100 + O-acetylserine (Thiol)-lyase, cysteine synthase (NCBI) False
RSP_2288 RSP_2288 CDS None chromosome 1 910229 911404 + hypothetical protein (NCBI) False
RSP_2289 RSP_2289 CDS None chromosome 1 911408 912985 + Outer membrane protein, OmpA/MotB family (NCBI) False
RSP_2502 glmS CDS None chromosome 1 1146147 1147958 - Glucosamine--fructose-6-phosphate aminotransferase (NCBI) False
RSP_2503 glmU CDS None chromosome 1 1147958 1149322 - Glucosamine-1-phosphate N-acetyltransferase; UDP-N-acetylglucosamine pyrophosphorylase (NCBI) False
RSP_2504 gph CDS None chromosome 1 1149423 1150112 + phosphoglycolate phosphatase (NCBI) False
RSP_2697 RSP_2697 CDS None chromosome 1 1343517 1344164 + hypothetical protein (NCBI) False
RSP_2781 RSP_2781 CDS None chromosome 1 1429456 1430004 - peroxiredoxin/glutaredoxin family protein (NCBI) False
RSP_2809 RSP_2809 CDS None chromosome 1 1505485 1506585 - ABC transporter, inner membrane subunit (NCBI) False
RSP_2852 RSP_2852 CDS None chromosome 1 1451545 1452189 + Predicted polyketide biosynthesis associated protein (NCBI) False
RSP_2959 gapB CDS None chromosome 1 1641318 1642319 + Glyceraldehyde 3-phosphate dehydrogenase B (GAPDH) (NCBI) False
RSP_2968 RSP_2968 CDS None chromosome 1 1652437 1653843 - Probable dihydrolipoamide dehydrogenase (NCBI) False
RSP_3455 RSP_3455 CDS None chromosome 2 524927 525784 + D-alanine aminotransferase (NCBI) False
RSP_3583 RSP_3583 CDS None chromosome 2 676618 677838 - Class I monoheme cytochrome c (NCBI) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.