Organism : Rhodobacter sphaeroides 2.4.1 | Module List:
Module 332 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 332

There are 16 regulatory influences for Module 332

Regulator Table (16)
Regulator Name Type
RSP_0527 tf
RSP_2838 tf
RSP_1225 tf
RSP_2572 tf
RSP_2200 tf
RSP_0722 tf
RSP_3621 tf
RSP_0415 tf
RSP_1952 tf
RSP_2888 tf
RSP_2867 tf
RSP_2130 tf
RSP_3317 tf
RSP_3042 tf
RSP_1139 tf
RSP_0087 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
8370 1.10e-08 caCtTGGCAcGccgccTGCTTct
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8371 8.70e+02 CttTgTcGGAtT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 332 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Metabolism kegg category 1.52e-03 8.01e-03 10/21
Energy Metabolism kegg subcategory 0.00e+00 1.00e-06 9/21
Nitrogen metabolism kegg pathway 0.00e+00 0.00e+00 9/21
Xenobiotics Biodegradation and Metabolism kegg subcategory 3.39e-04 2.91e-03 3/21
Chloroalkane and chloroalkene degradation kegg pathway 2.00e-06 4.40e-05 3/21

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Central intermediary metabolism tigr mainrole 0.00e+00 0.00e+00 8/21
Nitrogen fixation tigr sub1role 0.00e+00 0.00e+00 8/21
Energy metabolism tigr mainrole 6.00e-06 1.60e-05 6/21
Electron transport tigr sub1role 0.00e+00 0.00e+00 6/21

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Metabolism cog category 5.00e-04 9.79e-04 14/21
Energy production and conversion cog subcategory 0.00e+00 0.00e+00 11/21
Nitrogenase molybdenum-iron protein, alpha and beta chains cog 0.00e+00 0.00e+00 4/21
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 332

There are 21 genes in Module 332

Gene Member Table (21)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
RSP_0528 nifW CDS None chromosome 1 2266028 2266354 - nitrogen fixation protein NifW (NCBI) False
RSP_0529 nifV CDS None chromosome 1 2266463 2267539 - Homocitrate synthase (NCBI) False
RSP_0530 nifS CDS None chromosome 1 2267539 2268702 - Cysteine desulfurase (NCBI) False
RSP_0531 nifU CDS None chromosome 1 2268702 2269442 - Nitrogen fixation protein (NCBI) False
RSP_0532 RSP_0532 CDS None chromosome 1 2269452 2269772 - hypothetical protein (NCBI) False
RSP_0535 RSP_0535 CDS None chromosome 1 2271272 2271742 - hypothetical protein (NCBI) False
RSP_0536 nifX CDS None chromosome 1 2271739 2272215 - putative nitrogenase molybdenum-iron protein NifX (NCBI) False
RSP_0537 nifN CDS None chromosome 1 2272187 2273554 - nitrogenase molybdenum-iron protein beta chain, nifN (NCBI) False
RSP_0538 nifE CDS None chromosome 1 2273554 2275017 - nifE, nitrogenase molybdenum-cofactor synthesis protein (NCBI) False
RSP_0539 nifK CDS None chromosome 1 2275264 2276784 - nitrogenase molybdenum-iron protein beta chain (NCBI) False
RSP_0540 nifD CDS None chromosome 1 2276885 2278366 - Nitrogenase iron-molybdenum protein, alpha chain (NCBI) False
RSP_0541 nifH CDS None chromosome 1 2278428 2279303 - Nitrogenase iron protein (Nitrogenase component II) (NCBI) False
RSP_0546 nifB CDS None chromosome 1 2282039 2283514 - NifB family--FeMo cofactor biosynthesis protein (NCBI) False
RSP_3189 RSP_3189 CDS None chromosome 2 243883 244080 - 4Fe-4S ferredoxin (NCBI) False
RSP_3192 rnfA CDS None chromosome 2 246232 246813 + Predicted NADH-ubiquinone oxidoreductase; subunit RnfA (NCBI) False
RSP_3193 rnfB CDS None chromosome 2 246833 247399 + Electron transport complex protein rnfB (NCBI) False
RSP_3194 rnfC CDS None chromosome 2 247396 248949 + 4Fe-4S ferredoxin, RnfC (NCBI) False
RSP_3195 rnfD CDS None chromosome 2 248946 250034 + RnfD protein (NCBI) False
RSP_3196 rnfG CDS None chromosome 2 250031 250690 + Electron transport complex, RnfABCDGE type, G subunit (NCBI) False
RSP_3197 rnfE CDS None chromosome 2 250706 251440 + rnfE protein precursor (NCBI) False
RSP_3198 rnfH CDS None chromosome 2 251437 251694 + probable rnfH protein (NCBI) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.