Organism : Rhodobacter sphaeroides 2.4.1 | Module List:
Module 48 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 48

There are 8 regulatory influences for Module 48

Regulator Table (8)
Regulator Name Type
RSP_0958 tf
RSP_1139 tf
RSP_2610 tf
RSP_1669 tf
RSP_3694 tf
RSP_0316 tf
RSP_3124 tf
RSP_2425 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
7816 1.20e-02 TTTtGaaTtt
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7817 5.60e-02 TTta.c.g.ttgAaAagacGa
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 48 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Genetic Information Processing kegg category 4.28e-04 3.47e-03 7/30
Translation kegg subcategory 1.78e-02 3.87e-02 3/30
Replication and Repair kegg subcategory 5.85e-04 4.30e-03 3/30
Homologous recombination kegg pathway 1.10e-05 1.88e-04 3/30

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
DNA metabolism tigr mainrole 1.05e-04 2.12e-04 4/30
DNA replication, recombination, and repair tigr sub1role 6.50e-05 1.35e-04 4/30

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Replication, recombination and repair cog subcategory 1.79e-03 3.12e-03 4/30
Function unknown cog subcategory 6.50e-03 1.07e-02 6/30
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 48

There are 30 genes in Module 48

Gene Member Table (30)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
RSP_0002 spbB CDS None chromosome 1 1704125 1704520 - Histone-like nucleoid-structuring protein H-NS (NCBI) False
RSP_0019 RSP_0019 CDS None chromosome 1 1721018 1721245 + hypothetical protein (NCBI) False
RSP_0196 clpX CDS None chromosome 1 1911210 1912475 - ATP-dependent protease Clp, ATPase subunit (NCBI) False
RSP_0197 clpP CDS None chromosome 1 1912600 1913232 - Protease subunit of ATP-dependent Clp proteases (NCBI) False
RSP_0555 ruvA CDS None chromosome 1 2292945 2293619 - Holliday junction DNA helicase ruvA (NCBI) False
RSP_0556 ruvC CDS None chromosome 1 2293616 2294122 - Holliday junction nuclease RuvC (NCBI) False
RSP_0557 RSP_0557 CDS None chromosome 1 2294442 2294654 + hypothetical protein (NCBI) False
RSP_0717 RSP_0717 CDS None chromosome 1 2463336 2464028 - hypothetical protein (NCBI) False
RSP_0718 rpsU CDS None chromosome 1 2464235 2464441 + 30S ribosomal protein S21 (RpsU) (NCBI) False
RSP_1383 RSP_1383 CDS None chromosome 1 3162164 3162694 - hypothetical protein (NCBI) False
RSP_1517 spbA CDS None chromosome 1 104024 104338 - Histone-like protein of HNS family (NCBI) False
RSP_1624 RSP_1624 CDS None chromosome 1 221252 221896 - hypothetical protein (NCBI) False
RSP_1741 RSP_1741 CDS None chromosome 1 322519 323130 + Possible LuxR family protein (NCBI) True
RSP_1772 accA CDS None chromosome 1 356475 357434 + Acetyl-CoA carboxylase, alpha subunit (NCBI) False
RSP_1793 RSP_1793 CDS None chromosome 1 379339 379860 - Single-strand DNA binding protein (NCBI) False
RSP_1864 RSP_1864 CDS None chromosome 1 463014 463232 + hypothetical protein (NCBI) False
RSP_1985 RSP_1985 CDS None chromosome 1 580691 581134 - hypothetical protein (NCBI) False
RSP_2175 RSP_2175 CDS None chromosome 1 778440 779180 - ABC transporter, ATPase subunit (NCBI) False
RSP_2540 tatA CDS None chromosome 1 1184186 1184398 + twin-arginine translocation system protein, TatA (NCBI) False
RSP_2625 nifU CDS None chromosome 1 1265057 1265497 - NifU-related protein involved in Fe-S cluster formation (NCBI) False
RSP_2674 RSP_2674 CDS None chromosome 1 1317485 1317832 - hypothetical protein (NCBI) False
RSP_2763 RSP_2763 CDS None chromosome 1 1410065 1410313 + hypothetical protein (NCBI) False
RSP_2764 RSP_2764 CDS None chromosome 1 1410310 1410615 + hypothetical protein (NCBI) False
RSP_2773 RSP_2773 CDS None chromosome 1 1420507 1421145 - hypothetical protein (NCBI) False
RSP_2776 acpP CDS None chromosome 1 1424176 1424442 + Acyl carrier protein, AcpP (NCBI) False
RSP_3007 RSP_3007 CDS None chromosome 2 42204 42785 - hypothetical protein (NCBI) False
RSP_3589 ihfB CDS None chromosome 2 681250 681531 - Integration host factor beta-subunit (NCBI) False
RSP_3634 RSP_3634 CDS None chromosome 2 743884 744174 - hypothetical protein (NCBI) False
RSP_4301 RSP_4301 tRNA None chromosome 1 512637 512720 + tRNA-Leu (NCBI) False
RSP_4314 RSP_4314 tRNA None chromosome 1 1942439 1942528 - tRNA-Ser (NCBI) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.