Organism : Clostridium acetobutylicum | Module List :
CAC3472

Protein containing transcriptional regulator domain (NCBI ptt file)

CircVis
Functional Annotations (1)
Function System
sequence-specific DNA binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC3472
(Mouseover regulator name to see its description)

CAC3472 is regulated by 17 influences and regulates 29 modules.
Regulators for CAC3472 (17)
Regulator Module Operator
CAC0189 357 tf
CAC0254 357 tf
CAC0255 357 tf
CAC0445 357 tf
CAC0977 357 tf
CAC1675 357 tf
CAC2236 357 tf
CAC2471 357 tf
CAC3429 357 tf
CAC3472 357 tf
CAC0144 349 tf
CAC0240 349 tf
CAC1928 349 tf
CAC2471 349 tf
CAC3271 349 tf
CAC3525 349 tf
CAC3646 349 tf
Regulated by CAC3472 (29)
Module Residual Genes
3 0.41 26
46 0.24 12
65 0.47 22
108 0.36 24
111 0.35 19
125 0.29 16
141 0.32 19
144 0.32 18
145 0.29 18
161 0.25 15
165 0.36 21
171 0.27 16
190 0.46 30
202 0.35 20
220 0.24 17
223 0.32 20
226 0.29 16
230 0.36 19
240 0.45 26
243 0.31 15
268 0.34 18
288 0.33 19
295 0.37 22
307 0.25 13
333 0.39 21
343 0.40 24
347 0.30 15
357 0.31 14
361 0.31 20
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7350 8.40e+02 CcgTTGat..tcc
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7351 5.00e+02 AAAgGAgaatG
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7366 2.40e-04 tGgAGGgt
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7367 2.10e+04 aGAGCG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC3472

CAC3472 is enriched for 1 functions in 2 categories.
Enrichment Table (1)
Function System
sequence-specific DNA binding go/ molecular_function
Module neighborhood information for CAC3472

CAC3472 has total of 31 gene neighbors in modules 349, 357
Gene neighbors (31)
Gene Common Name Description Module membership
CAC0240 CAC0240 Response regulator (CheY-like recever domain and HTH DNA-binding domain) (NCBI ptt file) 210, 349
CAC0346 CAC0346 Hypothetical protein (NCBI ptt file) 210, 349
CAC0396 CAC0396 3-hexulose-6-phosphate synthase (D-arabino-3-hexulose-6-phosphate formaldehyde lyase) (NCBI ptt file) 343, 357
CAC0674 CAC0674 L-serine dehydratase, alpha chain (NCBI ptt file) 19, 357
CAC1071 CAC1071 Transcriptional regulator, AcrR family (NCBI ptt file) 65, 357
CAC1366 CAC1366 Predicted membrane protein (NCBI ptt file) 234, 357
CAC1519 CAC1519 Predicted permease (NCBI ptt file) 253, 357
CAC1636 CAC1636 Uncharacterized protein, homolog of B. firmus (2654481) (NCBI ptt file) 291, 349
CAC1637 CAC1637 Hypothetical protein, CF-12 family (NCBI ptt file) 44, 349
CAC1638 CAC1638 Hypothetical protein, CF-37 family(almost identical) (NCBI ptt file) 291, 349
CAC1639 CAC1639 Hypothetical protein, CF-7 family (NCBI ptt file) 291, 349
CAC1640 CAC1640 Uncharacterized protein, YQCG B.subtilis homolog; CF-7 family (NCBI ptt file) 291, 349
CAC1773 sacC Levanase (NCBI ptt file) 98, 357
CAC1863 CAC1863 Hypothetical protein (NCBI ptt file) 336, 357
CAC2420 CAC2420 Methyl-accepting chemotaxis protein (NCBI ptt file) 65, 357
CAC2551 CAC2551 RNAse H family protein (NCBI ptt file) 19, 349
CAC2557 CAC2557 Hypothetical protein (NCBI ptt file) 223, 349
CAC2932 CAC2932 ABC-type MDR transporter, ATPase component (NCBI ptt file) 223, 349
CAC2970 PatB PLP-dependent aminotransferase, (NCBI ptt file) 337, 357
CAC3407 CAC3407 Acyl-CoA thioesterase 1 (NCBI ptt file) 46, 357
CAC3419 CAC3419 S-adenosylmethionine-dependent methyltransferase (NCBI ptt file) 220, 357
CAC3472 CAC3472 Protein containing transcriptional regulator domain (NCBI ptt file) 349, 357
CAC3511 CAC3511 Membrane associated methyl-accepting chemotaxis protein with HAMP domain (NCBI ptt file) 210, 357
CAC3525 CAC3525 Uncharacterized conserved protein, YjeB/RRF2 family (NCBI ptt file) 223, 349
CAC3534 CAC3534 Site-specific modification DNA-methyltransferase (NCBI ptt file) 120, 349
CAC3655 CAC3655 Heavy-metal transporting P-type ATPase (NCBI ptt file) 108, 357
CAC3703 CAC3703 Hypothetical protein (NCBI ptt file) 44, 349
CAC3705 CAC3705 Hypothetical protein, CF-37 family(almost identical) (NCBI ptt file) 44, 349
CAC3706 CAC3706 Hypothetical protein, CF-12 family (NCBI ptt file) 44, 349
CAC3707 CAC3707 Uncharacterized protein, homolog of Bacillus firmus (gi:2654481) (NCBI ptt file) 44, 349
CAC3713 CAC3713 Uncharacterized conserved protein, yukE/yfjA B.subtilis family (NCBI ptt file) 44, 349
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC3472
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend