Organism : Geobacter sulfurreducens | Module List :
GSU2753

sigma-54 dependent DNA-binding response regulator (VIMSS)

CircVis
Functional Annotations (9)
Function System
Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains cog/ cog
two-component response regulator activity go/ molecular_function
two-component signal transduction system (phosphorelay) go/ biological_process
sequence-specific DNA binding transcription factor activity go/ molecular_function
ATP binding go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
transcription factor binding go/ molecular_function
nucleoside-triphosphatase activity go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU2753
(Mouseover regulator name to see its description)

GSU2753 is regulated by 19 influences and regulates 49 modules.
Regulators for GSU2753 (19)
Regulator Module Operator
GSU0187 3 tf
GSU0581 3 tf
GSU0770 3 tf
GSU1542 3 tf
GSU1569 3 tf
GSU2033 3 tf
GSU2202 3 tf
GSU2716 3 tf
GSU2753 3 tf
GSU3217 3 tf
GSU3298 3 tf
GSU3457 3 tf
GSU0018 100 tf
GSU0205 100 tf
GSU0732 100 tf
GSU1483 100 tf
GSU2753 100 tf
GSU2964 100 tf
GSU3087 100 tf
Regulated by GSU2753 (49)
Module Residual Genes
3 0.47 20
7 0.54 19
14 0.36 20
23 0.41 22
30 0.50 27
31 0.38 18
33 0.41 20
40 0.46 23
41 0.29 17
49 0.44 26
65 0.49 25
83 0.40 14
97 0.40 26
100 0.40 21
102 0.32 19
105 0.45 27
106 0.42 21
119 0.49 18
130 0.50 30
132 0.33 14
133 0.50 24
138 0.43 17
143 0.28 16
144 0.30 21
160 0.53 32
162 0.44 18
163 0.34 18
167 0.22 12
170 0.40 17
184 0.57 21
187 0.30 18
194 0.47 13
218 0.32 18
221 0.41 22
234 0.40 24
237 0.32 17
240 0.35 17
243 0.48 19
246 0.34 17
248 0.49 28
249 0.43 26
256 0.43 21
295 0.44 29
299 0.26 18
310 0.45 19
316 0.29 16
326 0.42 18
329 0.39 16
332 0.26 14
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2166 1.50e-06 cagcTttcaCgaAcCaTtTta
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2167 3.50e-05 cCgTTGACccCcgCgGgcctatTc
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2360 2.00e+03 gGgAGA
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2361 9.00e+03 TTtATCGTGgGAA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU2753

GSU2753 is enriched for 9 functions in 3 categories.
Module neighborhood information for GSU2753

GSU2753 has total of 40 gene neighbors in modules 3, 100
Gene neighbors (40)
Gene Common Name Description Module membership
GSU0079 GSU0079 transcriptional regulator, Cro/CI family (VIMSS) 3, 18
GSU0080 degQ protease degQ (NCBI) 3, 119
GSU0081 GSU0081 hypothetical protein (VIMSS) 3, 127
GSU0083 GSU0083 conserved hypothetical protein TIGR00726 (VIMSS) 3, 40
GSU0084 GSU0084 aminotransferase, classes I and II (NCBI) 3, 237
GSU0239 GSU0239 conserved hypothetical protein (NCBI) 3, 119
GSU0240 GSU0240 malonyl Coa-acyl carrier protein transacylase, putative (VIMSS) 3, 119
GSU0242 acpP-1 acyl carrier protein (NCBI) 3, 119
GSU0246 GSU0246 polysaccharide deacetylase domain protein (VIMSS) 100, 119
GSU0247 GSU0247 radical SAM domain protein (NCBI) 3, 119
GSU0248 GSU0248 BchE/P-methylase family protein (VIMSS) 3, 119
GSU0485 GSU0485 conserved hypothetical protein (VIMSS) 100, 194
GSU0583 GSU0583 methyl-accepting chemotaxis protein (VIMSS) 100, 157
GSU0584 GSU0584 hypothetical protein (NCBI) 84, 100
GSU0684 cheW-2 purine-binding chemotaxis protein CheW (NCBI) 100, 234
GSU0969 ctpA-1 carboxy-terminal processing protease (NCBI) 3, 128
GSU1059 sucD succinyl-CoA synthase, alpha subunit (NCBI) 100, 326
GSU1212 GSU1212 conserved hypothetical protein (VIMSS) 3, 123
GSU2044 GSU2044 sensory box/GGDEF family protein (VIMSS) 100, 264
GSU2263 GSU2263 oxidoreductase, Gfo/Idh/MocA family (VIMSS) 3, 28
GSU2437 GSU2437 conserved hypothetical protein (VIMSS) 3, 187
GSU2681 GSU2681 iron-sulfur cluster-binding protein (VIMSS) 100, 320
GSU2753 GSU2753 sigma-54 dependent DNA-binding response regulator (VIMSS) 3, 100
GSU2816 GSU2816 sensory box histidine kinase/response regulator (VIMSS) 72, 100
GSU3057 gltA glutamate synthase (NADPH), homotetrameric (NCBI) 100, 145
GSU3114 GSU3114 hypothetical protein (VIMSS) 4, 100
GSU3115 GSU3115 hypothetical protein (VIMSS) 100, 234
GSU3130 GSU3130 lipoprotein, putative (VIMSS) 100, 326
GSU3138 GSU3138 sensor histidine kinase/response regulator (VIMSS) 100, 326
GSU3140 GSU3140 peptidase, M1 family protein (NCBI) 4, 100
GSU3200 GSU3200 chemotaxis protein, CheC family (NCBI) 100, 326
GSU3240 GSU3240 radical SAM domain protein (NCBI) 100, 264
GSU3253 GSU3253 response regulator (VIMSS) 100, 279
GSU3254 mpg phosphoglucomutase/phosphomannomutase family protein (NCBI) 100, 256
GSU3255 GSU3255 conserved hypothetical protein (NCBI) 100, 279
GSU3333 GSU3333 3-deoxy-7-phosphoheptulonate synthase (RefSeq) 3, 119
GSU3334 GSU3334 cytochrome c family protein, putative (NCBI) 3, 205
GSU3335 GSU3335 hypothetical protein (VIMSS) 3, 333
GSU3336 GSU3336 hypothetical protein (NCBI) 3, 160
GSU3408 GSU3408 L-threonine aldolase, low-specificity (VIMSS) 3, 28
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU2753
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend