Organism : Geobacter sulfurreducens | Module List:
Module 53 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 53

There are 9 regulatory influences for Module 53

Regulator Table (9)
Regulator Name Type
GSU1218 tf
GSU2945 tf
GSU1525 tf
GSU1989 tf
GSU3089 tf
GSU1992 tf
GSU0732 tf
GSU1692 tf
GSU1320 tf

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
2266 6.10e+02 taaaGTTctTacagg
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2267 2.40e+03 aAAATCAaaCaAcTgC.AA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 53 is enriched for following functions.

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Regulatory functions tigr mainrole 2.55e-03 4.46e-03 3/28
Transport and binding proteins tigr mainrole 2.82e-03 4.88e-03 3/28
Unknown substrate tigr sub1role 1.00e-05 3.20e-05 3/28

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Cellular processes and signaling cog category 3.11e-03 5.94e-03 12/28
Signal transduction mechanisms cog subcategory 3.42e-04 7.45e-04 8/28
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 53

There are 28 genes in Module 53

Gene Member Table (28)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
GSU0144 GSU0144 CDS None chromosome 158787 160916 + sensory box histidine kinase (VIMSS) False
GSU0304 pepN CDS None chromosome 334411 337044 - aminopeptidase N (NCBI) False
GSU0500 typA CDS None chromosome 533056 534852 + GTP-binding protein TypA (NCBI) False
GSU0657 GSU0657 CDS None chromosome 692974 693240 + hypothetical protein (VIMSS) False
GSU0681 GSU0681 CDS None chromosome 716939 718009 + sensory box histidine kinase (VIMSS) False
GSU0827 GSU0827 CDS None chromosome 887079 887483 + hypothetical protein (VIMSS) False
GSU0829 GSU0829 CDS None chromosome 888914 890185 + heavy metal efflux pump, CzcB family (VIMSS) False
GSU1036 GSU1036 CDS None chromosome 1119359 1121098 - sensor histidine kinase (VIMSS) False
GSU1414 GSU1414 CDS None chromosome 1551250 1553784 + sensory box histidine kinase/response regulator (VIMSS) False
GSU1427 GSU1427 CDS None chromosome 1563855 1564190 - anti-anti-sigma factor (VIMSS) False
GSU1430 GSU1430 CDS None chromosome 1567543 1567800 - hypothetical protein (VIMSS) False
GSU1642 GSU1642 CDS None chromosome 1801229 1801723 - conserved hypothetical protein (VIMSS) False
GSU1673 GSU1673 CDS None chromosome 1833619 1833879 - hypothetical protein (VIMSS) False
GSU1817 GSU1817 CDS None chromosome 1983011 1983580 + outer membrane lipoprotein, Slp family, putative (VIMSS) False
GSU1857 GSU1857 CDS None chromosome 2032030 2032995 - hypothetical protein (VIMSS) False
GSU1989 GSU1989 CDS None chromosome 2182011 2183345 - sigma-54 dependent DNA-binding response regulator (VIMSS) True
GSU1990 GSU1990 CDS None chromosome 2183375 2184382 - sensor histidine kinase (VIMSS) False
GSU2665 GSU2665 CDS None chromosome 2939179 2940339 - efflux transporter, RND family, MFP subunit (NCBI) False
GSU2666 GSU2666 CDS None chromosome 2940336 2940977 - transcriptional regulator, TetR family (VIMSS) True
GSU2668 GSU2668 CDS None chromosome 2942694 2942975 + hypothetical protein (VIMSS) False
GSU2669 GSU2669 CDS None chromosome 2942972 2944003 + sensor histidine kinase (VIMSS) False
GSU2678 hsp CDS None chromosome 2957582 2958037 + heat shock protein, Hsp20 family (NCBI) False
GSU2692 GSU2692 CDS None chromosome 2970258 2971247 - immunogenic protein family protein (VIMSS) False
GSU3086 GSU3086 CDS None chromosome 3391779 3392954 - conserved hypothetical protein (VIMSS) False
GSU3088 GSU3088 CDS None chromosome 3393821 3394303 - ybaK/ebsC protein (VIMSS) False
GSU3089 rpoD CDS None chromosome 3394412 3396145 - RNA polymerase sigma factor RpoD (NCBI) True
GSU3095 hisF CDS None chromosome 3399779 3400540 - imidazoleglycerol phosphate synthase, cyclase subunit (NCBI) False
GSU3139 GSU3139 CDS None chromosome 3446013 3446861 - conserved hypothetical protein (VIMSS) False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.