Organism : Methanococcus maripaludis S2 | Module List :
MMP1545 rplW

50S ribosomal protein L23P

CircVis
Functional Annotations (8)
Function System
Ribosomal protein L23 cog/ cog
nucleotide binding go/ molecular_function
RNA binding go/ molecular_function
structural constituent of ribosome go/ molecular_function
ribosome go/ cellular_component
translation go/ biological_process
Ribosome kegg/ kegg pathway
L23_arch tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for MMP1545
(Mouseover regulator name to see its description)

MMP1545 is regulated by 17 influences and regulates 0 modules.
Regulators for MMP1545 rplW (17)
Regulator Module Operator
MMP0209 56 tf
MMP0257
MMP1447
56 combiner
MMP0460 56 tf
MMP0568 56 tf
MMP0700
MMP1347
56 combiner
MMP1347 56 tf
MMP0052
MMP0097
128 combiner
MMP0052
MMP0257
128 combiner
MMP0460 128 tf
MMP0568 128 tf
MMP0993
MMP1646
128 combiner
MMP1015 128 tf
MMP0052 63 tf
MMP0052
MMP0257
63 combiner
MMP0460 63 tf
MMP0568 63 tf
MMP0907
MMP1646
63 combiner

Warning: MMP1545 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 6 motifs predicted.

Motif Table (6)
Motif Id e-value Consensus Motif Logo
773 1.50e+01 GGcGCg
Loader icon
774 1.30e+02 GGAGGt
Loader icon
787 5.10e+01 GcTaGggcc
Loader icon
788 1.30e+02 ACCTCC
Loader icon
909 1.00e+01 gAcgGG
Loader icon
910 2.10e+01 cGcacGcc
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for MMP1545

MMP1545 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Ribosomal protein L23 cog/ cog
nucleotide binding go/ molecular_function
RNA binding go/ molecular_function
structural constituent of ribosome go/ molecular_function
ribosome go/ cellular_component
translation go/ biological_process
Ribosome kegg/ kegg pathway
L23_arch tigr/ tigrfam
Module neighborhood information for MMP1545

MMP1545 has total of 21 gene neighbors in modules 56, 63, 128
Gene neighbors (21)
Gene Common Name Description Module membership
MMP0062 50S ribosomal protein L31e 61, 77, 96, 128, 138
MMP0156 30S ribosomal protein S19e 63, 79, 91
MMP0259 rplP0 acidic ribosomal protein P0 11, 18, 56
MMP0260 rpl1P 50S ribosomal protein L1P 18, 56
MMP0298 rpl15 50S ribosomal protein L15e 18, 128
MMP0626 cmk cytidylate kinase 18, 56
MMP0627 rpl34e 50S ribosomal protein L34e 18, 56, 79
MMP0952 aIF-5A translation initiation factor IF-5A 63, 130
MMP1148 small nuclear ribonucleoprotein 16, 63
MMP1207 30S ribosomal protein S6e 105, 128
MMP1360 rpoH DNA-directed RNA polymerase subunit H 63, 162
MMP1362 rpoB1 DNA-directed RNA polymerase subunit B' 63, 130, 140
MMP1371 rps10p 30S ribosomal protein S10P 63, 130
MMP1406 translation initiation factor Sui1 63, 158
MMP1543 rpl3p 50S ribosomal protein L3P 63, 92, 128
MMP1544 rpl4lp 50S ribosomal protein L4P 56, 63, 128
MMP1545 rplW 50S ribosomal protein L23P 56, 63, 128
MMP1546 rpl2p 50S ribosomal protein L2P 56, 128
MMP1547 rps19p 30S ribosomal protein S19P 56, 128
MMP1658 30S ribosomal protein S8e 63, 130
Unanno_1 None 63, 135
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for MMP1545
Please add your comments for this gene by using the form below. Your comments will be publicly available.

comments powered by Disqus

Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend