Rv0232 Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0232 Transcriptional regulator, TetR family CDS 277899 278588 + 690 229 TRUE

Rv0232 (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0089 with residual 0.51 and bicluster_0560 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.83 and 9.30 for bicluster_0089 and 0.01 and 21.00 for bicluster_0560 respectively.

These modules are enriched for following go terms: folic acid-containing compound biosynthe..., pteridine-containing compound biosynthet..., coenzyme metabolic process, cellular modified amino acid biosyntheti....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13500 MT0242 148
Displaying 1 - 2 of 2
Gene Target Differential Expression Distance Expression pvalue Type
No -28 0.81 0.0930744 CDS
Probable phiRV1 phage protein
No -44 -0.06 0.949829 CDS
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Mycofactocin system transcriptional regulator
No -107 -0.11 0.999588 Primary.TSS
No 51 0.54 0.313819 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN [PROBABLY TETR_ACRR-FAMILY] TetR/ACRR family transcriptional regulator
Operon # Operon
161 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607373 NP_214746.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426432 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426433 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426434 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426435 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426436 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0232_B139 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.320000 1.43

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00035723
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 11.16 U
D3I 3 3 3.83 11.22 I
D3U 3 3 3.83 10.57 U
D5I 9 5 6.00 10.77 I
D5U 17 5 6.00 10.44 U
D7I 18 7 8.14 11.41 I
D7U 19 7 8.14 10.44 U
D14I 4 14 15.63 9.81 I
D14U 4 14 15.63 8.41 U
D17I 3 17 19.15 8.94 I
D17U 3 17 19.15 7.30 U
D21U 4 21 23.23 7.75 U
D21I 4 21 23.23 8.91 I
D24I 3 24 26.60 8.03 I
D24U 3 24 26.60 6.46 U
D28I 4 28 30.61 8.25 I
D28U 4 28 30.61 6.87 U