Rv0452 Transcriptional regulator, TetR family

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0452 Transcriptional regulator, TetR family CDS 542142 542852 + 711 236 FALSE

Rv0452 (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0038 with residual 0.42 and bicluster_0049 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 5,200.00 and 7,700.00 for bicluster_0038 and 0.00 and 0.01 for bicluster_0049 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS Re-Annotated Start Tuberculist Annotated Start
-0.008 18 542160 542160 542142
Product (LegacyBRC) Product (RefSeq)
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN transcriptional regulatory protein
Operon # Operon
301
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607593 NP_214966.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 1.14

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00145768
p-value INH: 0.0727849
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 8.19 U
D7U 19 7 8.14 7.37 U
D7I 18 7 8.14 8.12 I
D5U 17 5 6.00 8.45 U
D5I 9 5 6.00 8.75 I
D14I 4 14 15.63 6.26 I
D14U 4 14 15.63 5.66 U
D21I 4 21 23.23 6.20 I
D21U 4 21 23.23 5.31 U
D28I 4 28 30.61 6.06 I
D28U 4 28 30.61 5.50 U
D17I 3 17 19.15 6.89 I
D17U 3 17 19.15 6.06 U
D24I 3 24 26.60 6.81 I
D24U 3 24 26.60 5.56 U
D3I 3 3 3.83 7.86 I
D3U 3 3 3.83 7.64 U