Rv0651 LSU ribosomal protein L10p (P0)
This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,200.00 and 5,400.00 for bicluster_0232 and 0.01 and 9.30 for bicluster_0396 respectively.
These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cell, cell part organic hydroxy compound transport, tetracycline transport, antibiotic transport, toxin transport, drug transport, response to drug, tetracycline transporter activity, drug:hydrogen antiporter activity, tetracycline:hydrogen antiporter activit..., toxin transporter activity, antibiotic transporter activity, organic hydroxy compound transmembrane t..., solute:cation antiporter activity, solute:hydrogen antiporter activity, antiporter activity.
This gene is found to be for growth on cholesterol.
|Product (LegacyBRC)||Product (RefSeq)|
|50S ribosomal protein L10||50S ribosomal protein L10|
|BioCyc Gene Page||Cellular Overview Map|
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.