Rv0891c Adenylyl cyclase class-3/4/guanylyl cyclase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0891c Adenylyl cyclase class-3/4/guanylyl cyclase CDS 992598 993455 - 858 285 TRUE

Rv0891c (Adenylyl cyclase class-3/4/guanylyl cyclase) is predicted to be co-regulated in modules bicluster_0115 with residual 0.48 and bicluster_0429 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 50.00 and 1,500.00 for bicluster_0115 and 1.40 and 2,100.00 for bicluster_0429 respectively.

These modules are enriched for following go terms: transferase activity, transferring one-c....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15107 MT0915 2345
Displaying 1 - 4 of 4
Gene Target Differential Expression Distance Expression pvalue Type
SSU ribosomal protein S13p (S18e)
No 40 0.13 0.723084 Primary.TSS
LSU ribosomal protein L36p
No 40 0 0.996691 Internal.TSS
LSU ribosomal protein L36p
No -10 0 0.996691 Internal.TSS
LSU ribosomal protein L36p
No -39 0 0.996691 CDS
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
No 59 0.47 0.205074 Primary.TSS
Possible oxidoreductase subunit
No 30 0.6 0.0896391 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0891c_MT0915 transcriptional regulatory protein
Operon # Operon
596 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608031 NP_215406.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426584 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426585 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426586 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0891c_B387 UCSC Browser Tracks
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
1.000000 10.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.0477108
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 13.37 U
D14I 4 14 15.63 11.81 I
D14U 4 14 15.63 11.66 U
D17I 3 17 19.15 10.81 I
D17U 3 17 19.15 10.79 U
D21I 4 21 23.23 10.79 I
D21U 4 21 23.23 11.02 U
D24I 3 24 26.60 9.40 I
D24U 3 24 26.60 9.88 U
D28I 4 28 30.61 9.79 I
D28U 4 28 30.61 10.25 U
D3I 3 3 3.83 12.84 I
D3U 3 3 3.83 13.14 U
D5I 9 5 6.00 13.87 I
D5U 17 5 6.00 13.83 U
D7I 18 7 8.14 13.08 I
D7U 19 7 8.14 12.77 U