Rv2351c Phospholipase C 3 PlcC (EC 3.1.4.3)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2351c plcA Phospholipase C 3 PlcC (EC 3.1.4.3) CDS 2630537 2632075 - 1 539 512 FALSE

Rv2351c (Phospholipase C 3 PlcC (EC 3.1.4.3)) is predicted to be co-regulated in modules bicluster_0062 with residual 0.51 and bicluster_0368 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 18.00 and 490.00 for bicluster_0062 and 430.00 and 1,000.00 for bicluster_0368 respectively.

These modules are enriched for following go terms: cellular component biogenesis, cellular component organization or bioge..., protein binding.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.433 2632099 2632075
Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-13468 MT2416 1435
Product (LegacyBRC) Product (RefSeq)
Phospholipase C 1 membrane-associated phospholipase C
Operon # Operon
1554
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Inositol phosphate metabolism

9
Total items in this category:  

KEGG

Glycerophospholipid metabolism

21
Total items in this category:  

KEGG

Ether lipid metabolism

7
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15609488 NP_216867.1 Run
GO:0004629

phospholipase C activity

phospholipase C activity

Details: 
Catalysis of the reaction: a phospholipid + H2O = 1,2-diacylglycerol + a phosphatidate.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0034480

phosphatidylcholine phospholipase C activity

phosphatidylcholine phospholipase C activity

Details: 
Catalysis of the reaction: phosphatidylcholine + H2O = 1,2-diacylglycerol + a choline phosphate.
GO Category: 
molecular_function
4
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.410000 1.20

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: