Rv3785 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv3785 dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) CDS 4231320 4232393 + 1 074 357 FALSE

Rv3785 (dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)) is predicted to be co-regulated in modules bicluster_0313 with residual 0.51 and bicluster_0332 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.60 and 0.91 for bicluster_0313 and 0.02 and 590.00 for bicluster_0332 respectively.

These modules are enriched for following go terms: purine nucleotide biosynthetic process, purine nucleotide metabolic process, purine-containing compound biosynthetic ....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv3785_MT3893
Operon # Operon
2478
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15610921 NP_218302.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.360000 0.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: