Organism : Pseudomonas aeruginosa | Module List :
PA0580 gcp

O-sialoglycoprotein endopeptidase (NCBI)

CircVis
Functional Annotations (5)
Function System
Metal-dependent proteases with possible chaperone activity cog/ cog
proteolysis go/ biological_process
zinc ion binding go/ molecular_function
O-sialoglycoprotein endopeptidase activity go/ molecular_function
gcp_kae1 tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0580
(Mouseover regulator name to see its description)

PA0580 is regulated by 37 influences and regulates 0 modules.
Regulators for PA0580 gcp (37)
Regulator Module Operator
PA0120 230 tf
PA0236 230 tf
PA0289 230 tf
PA1359 230 tf
PA1422 230 tf
PA2047 230 tf
PA2622 230 tf
PA2692 230 tf
PA2957 230 tf
PA3574 230 tf
PA3622 230 tf
PA3921 230 tf
PA4052 230 tf
PA4279 230 tf
PA4853 230 tf
PA4890 230 tf
PA5403 230 tf
PA5550 230 tf
PA0120 404 tf
PA0125 404 tf
PA1159 404 tf
PA1359 404 tf
PA1422 404 tf
PA1520 404 tf
PA2047 404 tf
PA2622 404 tf
PA2957 404 tf
PA3135 404 tf
PA3604 404 tf
PA3622 404 tf
PA4052 404 tf
PA4451 404 tf
PA4745 404 tf
PA4853 404 tf
PA5239 404 tf
PA5253 404 tf
PA5550 404 tf

Warning: PA0580 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3288 1.20e-05 TAaAaT.cgcccCctTttc
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3289 3.40e+03 ctTgGcAAAT
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3632 1.80e+00 TAagAT.cgCgaCCcTtgaa.acc
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3633 2.00e-01 AAaGCaaagcctccGAgCggAAA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0580

PA0580 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Metal-dependent proteases with possible chaperone activity cog/ cog
proteolysis go/ biological_process
zinc ion binding go/ molecular_function
O-sialoglycoprotein endopeptidase activity go/ molecular_function
gcp_kae1 tigr/ tigrfam
Module neighborhood information for PA0580

PA0580 has total of 26 gene neighbors in modules 230, 404
Gene neighbors (26)
Gene Common Name Description Module membership
PA0352 PA0352 probable transporter (NCBI) 39, 230
PA0559 PA0559 hypothetical protein (NCBI) 65, 230
PA0577 dnaG DNA primase (NCBI) 404, 474
PA0578 PA0578 hypothetical protein (NCBI) 404, 474
PA0580 gcp O-sialoglycoprotein endopeptidase (NCBI) 230, 404
PA0583 PA0583 hypothetical protein (NCBI) 135, 230
PA0858 PA0858 hypothetical protein (NCBI) 114, 404
PA1116 PA1116 hypothetical protein (NCBI) 230, 422
PA1299 PA1299 hypothetical protein (NCBI) 111, 230
PA1964 PA1964 probable ATP-binding component of ABC transporter (NCBI) 114, 230
PA2653 PA2653 probable transporter (NCBI) 65, 230
PA2840 PA2840 probable ATP-dependent RNA helicase (NCBI) 404, 412
PA2855 PA2855 hypothetical protein (NCBI) 230, 351
PA3179 PA3179 hypothetical protein (NCBI) 404, 528
PA3607 potA polyamine transport protein PotA (NCBI) 230, 314
PA3741 PA3741 hypothetical protein (NCBI) 404, 501
PA3818 PA3818 extragenic suppressor protein SuhB (NCBI) 230, 404
PA3824 queA S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) 404, 520
PA4544 rluD pseudouridine synthase (NCBI) 404, 424
PA4748 tpiA triosephosphate isomerase (NCBI) 404, 474
PA4840 PA4840 hypothetical protein (NCBI) 230, 255
PA4873 PA4873 probable heat-shock protein (NCBI) 135, 230
PA5118 thiI thiamine biosynthesis protein ThiI (NCBI) 56, 404
PA5296 rep ATP-dependent DNA helicase Rep (NCBI) 106, 230
PA5406 PA5406 hypothetical protein (NCBI) 65, 230
PA5407 PA5407 hypothetical protein (NCBI) 65, 230
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0580
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend