Organism : Pseudomonas aeruginosa | Module List :
PA1375 pdxB

erythronate-4-phosphate dehydrogenase (NCBI)

CircVis
Functional Annotations (7)
Function System
Phosphoglycerate dehydrogenase and related dehydrogenases cog/ cog
metabolic process go/ biological_process
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor go/ molecular_function
4-phosphoerythronate dehydrogenase activity go/ molecular_function
NAD binding go/ molecular_function
Vitamin B6 metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1375
(Mouseover regulator name to see its description)

PA1375 is regulated by 39 influences and regulates 0 modules.
Regulators for PA1375 pdxB (39)
Regulator Module Operator
PA0236 490 tf
PA0456 490 tf
PA1754 490 tf
PA1853 490 tf
PA2047 490 tf
PA2093 490 tf
PA2447 490 tf
PA2497 490 tf
PA2921 490 tf
PA2957 490 tf
PA3007 490 tf
PA3322 490 tf
PA4275 490 tf
PA4451 490 tf
PA4745 490 tf
PA4764 490 tf
PA4769 490 tf
PA4853 490 tf
PA5125 490 tf
PA5344 490 tf
PA5438 490 tf
PA5483 490 tf
PA0037 351 tf
PA0152 351 tf
PA0253 351 tf
PA0831 351 tf
PA1241 351 tf
PA1290 351 tf
PA1630 351 tf
PA2115 351 tf
PA2270 351 tf
PA2447 351 tf
PA2510 351 tf
PA2921 351 tf
PA3322 351 tf
PA3604 351 tf
PA4451 351 tf
PA4890 351 tf
PA5356 351 tf

Warning: PA1375 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3526 2.00e-03 CgcCG.TcCgcCAt.CcttgtaG
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3527 6.60e+00 tTcG.CaAcGC
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3794 1.30e+02 tTttCgaGacAatcCcat
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3795 2.20e+01 tCGcCcTtcTaGagt
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1375

PA1375 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Phosphoglycerate dehydrogenase and related dehydrogenases cog/ cog
metabolic process go/ biological_process
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor go/ molecular_function
4-phosphoerythronate dehydrogenase activity go/ molecular_function
NAD binding go/ molecular_function
Vitamin B6 metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for PA1375

PA1375 has total of 43 gene neighbors in modules 351, 490
Gene neighbors (43)
Gene Common Name Description Module membership
PA0152 pcaQ transcriptional regulator PcaQ (NCBI) 351, 536
PA0253 PA0253 probable transcriptional regulator (NCBI) 181, 351
PA0308 PA0308 hypothetical protein (NCBI) 220, 490
PA0350 folA dihydrofolate reductase (NCBI) 58, 351
PA0463 creB two-component response regulator CreB (NCBI) 58, 351
PA0464 creC two-component sensor CreC (NCBI) 351, 507
PA0760 PA0760 hypothetical protein (NCBI) 135, 490
PA0789 PA0789 probable amino acid permease (NCBI) 100, 490
PA1126 PA1126 hypothetical protein (NCBI) 265, 490
PA1332 PA1332 hypothetical protein (NCBI) 108, 351
PA1375 pdxB erythronate-4-phosphate dehydrogenase (NCBI) 351, 490
PA1853 PA1853 probable transcriptional regulator (NCBI) 117, 490
PA1965 PA1965 hypothetical protein (NCBI) 139, 351
PA2270 PA2270 probable transcriptional regulator (NCBI) 257, 351
PA2450 PA2450 hypothetical protein (NCBI) 139, 351
PA2502 PA2502 hypothetical protein (NCBI) 135, 351
PA2503 PA2503 hypothetical protein (NCBI) 351, 490
PA2510 catR transcriptional regulator CatR (NCBI) 307, 351
PA2855 PA2855 hypothetical protein (NCBI) 230, 351
PA2921 PA2921 probable transcriptional regulator (NCBI) 61, 351
PA2942 PA2942 probable magnesium chelatase (NCBI) 58, 490
PA2982 PA2982 hypothetical protein (NCBI) 387, 490
PA2983 PA2983 probable tolQ-type transport protein (NCBI) 143, 490
PA3208 PA3208 hypothetical protein (NCBI) 8, 490
PA3388 PA3388 hypothetical protein (NCBI) 299, 351
PA3397 fpr ferredoxin--NADP+ reductase (NCBI) 371, 490
PA3473 PA3473 hypothetical protein (NCBI) 351, 377
PA3474 PA3474 hypothetical protein (NCBI) 351, 377
PA3537 argF ornithine carbamoyltransferase, anabolic (NCBI) 276, 490
PA3794 PA3794 hypothetical protein (NCBI) 139, 490
PA3887 nhaP Na+/H+ antiporter NhaP (NCBI) 220, 490
PA3949 PA3949 hypothetical protein (NCBI) 351, 544
PA4013 PA4013 hypothetical protein (NCBI) 351, 422
PA4014 PA4014 hypothetical protein (NCBI) 58, 351
PA4634 PA4634 hypothetical protein (NCBI) 299, 351
PA4640 mqoB malate:quinone oxidoreductase (NCBI) 398, 490
PA4789 PA4789 hypothetical protein (NCBI) 229, 351
PA4790 PA4790 hypothetical protein (NCBI) 229, 351
PA4801 PA4801 hypothetical protein (NCBI) 299, 490
PA5150 PA5150 probable short-chain dehydrogenase (NCBI) 190, 351
PA5151 PA5151 hypothetical protein (NCBI) 190, 351
PA5252 PA5252 probable ATP-binding component of ABC transporter (NCBI) 151, 490
PA5351 rubA1 Rubredoxin 1 (NCBI) 117, 490
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1375
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend