Organism : Pseudomonas aeruginosa | Module List :
PA1473

hypothetical protein (NCBI)

CircVis
Functional Annotations (3)
Function System
Uncharacterized homolog of the cytoplasmic domain of flagellar protein FhlB cog/ cog
protein secretion go/ biological_process
membrane go/ cellular_component
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1473
(Mouseover regulator name to see its description)

PA1473 is regulated by 29 influences and regulates 0 modules.
Regulators for PA1473 (29)
Regulator Module Operator
PA0034 522 tf
PA0179 522 tf
PA0225 522 tf
PA0289 522 tf
PA0491 522 tf
PA0707 522 tf
PA0893 522 tf
PA1097 522 tf
PA1229 522 tf
PA1430 522 tf
PA1526 522 tf
PA2586 522 tf
PA2588 522 tf
PA2879 522 tf
PA3002 522 tf
PA3599 522 tf
PA3689 522 tf
PA4547 522 tf
PA5344 522 tf
PA0191 3 tf
PA0367 3 tf
PA0393 3 tf
PA0707 3 tf
PA0791 3 tf
PA1760 3 tf
PA3458 3 tf
PA4354 3 tf
PA4547 3 tf
PA5562 3 tf

Warning: PA1473 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2842 7.10e+02 TTTttCcg
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2843 4.30e+04 AA.GT.GTttTcCTA
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3858 6.10e+01 GtAAcagcctgt.TTacaCttcCC
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3859 4.50e+03 TGGATAAaA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1473

PA1473 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Uncharacterized homolog of the cytoplasmic domain of flagellar protein FhlB cog/ cog
protein secretion go/ biological_process
membrane go/ cellular_component
Module neighborhood information for PA1473

PA1473 has total of 50 gene neighbors in modules 3, 522
Gene neighbors (50)
Gene Common Name Description Module membership
PA0416 chpD probable transcriptional regulator (NCBI) 3, 321
PA0457 PA0457 hypothetical protein (NCBI) 3, 550
PA0458 PA0458 probable major facilitator superfamily (MFS) transporter (NCBI) 3, 443
PA0597 PA0597 probable nucleotidyl transferase (NCBI) 3, 452
PA0598 PA0598 hypothetical protein (NCBI) 452, 522
PA0917 kup potassium uptake protein Kup (NCBI) 3, 499
PA1039 PA1039 hypothetical protein (NCBI) 3, 338
PA1042 PA1042 hypothetical protein (NCBI) 3, 384
PA1208 PA1208 hypothetical protein (NCBI) 3, 432
PA1460 motC flagellar motor protein (NCBI) 129, 522
PA1461 motD flagellar motor protein (NCBI) 129, 522
PA1473 PA1473 hypothetical protein (NCBI) 3, 522
PA1526 PA1526 probable transcriptional regulator (NCBI) 62, 522
PA1527 PA1527 hypothetical protein (NCBI) 202, 522
PA2231 pslA probable glycosyl transferase (NCBI) 300, 522
PA2232 pslB probable phosphomannose isomerase/GDP-mannose pyrophosphorylase (NCBI) 300, 522
PA2233 pslC probable glycosyl transferase (NCBI) 300, 522
PA2234 pslD probable exopolysaccharide transporter (NCBI) 300, 522
PA2235 pslE hypothetical protein (NCBI) 300, 522
PA2236 pslF hypothetical protein (NCBI) 300, 522
PA2237 pslG probable glycosyl hydrolase (NCBI) 300, 522
PA2238 pslH hypothetical protein (NCBI) 300, 522
PA2239 pslI probable transferase (NCBI) 300, 522
PA2240 pslJ hypothetical protein (NCBI) 300, 522
PA2241 pslK hypothetical protein (NCBI) 289, 522
PA2242 pslL hypothetical protein (NCBI) 300, 522
PA2706 PA2706 hypothetical protein (NCBI) 3, 185
PA3020 PA3020 probable soluble lytic transglycosylase (NCBI) 62, 522
PA3087 PA3087 hypothetical protein (NCBI) 3, 253
PA3138 uvrB excinuclease ABC subunit B (NCBI) 3, 87
PA3300 fadD2 long-chain-fatty-acid--CoA ligase (NCBI) 3, 163
PA3707 wspB hypothetical protein (NCBI) 3, 388
PA3739 PA3739 probable sodium/hydrogen antiporter (NCBI) 3, 85
PA3750 PA3750 hypothetical protein (NCBI) 3, 253
PA3797 PA3797 hypothetical protein (NCBI) 3, 88
PA4016 PA4016 hypothetical protein (NCBI) 3, 321
PA4199 PA4199 probable acyl-CoA dehydrogenase (NCBI) 3, 443
PA4200 PA4200 hypothetical protein (NCBI) 3, 203
PA4388 PA4388 hypothetical protein (NCBI) 3, 320
PA4400 PA4400 hypothetical protein (NCBI) 3, 384
PA4401 PA4401 probable glutathione S-transferase (NCBI) 3, 384
PA4476 PA4476 hypothetical protein (NCBI) 3, 28
PA4604 PA4604 hypothetical protein (NCBI) 3, 511
PA4618 PA4618 hypothetical protein (NCBI) 3, 517
PA5014 glnE glutamate-ammonia-ligase adenylyltransferase (NCBI) 3, 62
PA5198 PA5198 LD-carboxypeptidase (NCBI) 3, 253
PA5221 PA5221 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (NCBI) 3, 499
PA5229 PA5229 hypothetical protein (NCBI) 3, 203
PA5302 dadX catabolic alanine racemase (NCBI) 3, 517
PA5447 wbpZ glycosyltransferase WbpZ (NCBI) 3, 469
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1473
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend