Organism : Pseudomonas aeruginosa | Module List :
PA4077

probable transcriptional regulator (NCBI)

CircVis
Functional Annotations (2)
Function System
Predicted transcriptional regulators cog/ cog
sequence-specific DNA binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4077
(Mouseover regulator name to see its description)

PA4077 is regulated by 42 influences and regulates 8 modules.
Regulators for PA4077 (42)
Regulator Module Operator
PA0123 58 tf
PA0152 58 tf
PA0225 58 tf
PA0831 58 tf
PA1627 58 tf
PA2076 58 tf
PA2877 58 tf
PA3322 58 tf
PA3604 58 tf
PA3898 58 tf
PA4057 58 tf
PA4077 58 tf
PA4451 58 tf
PA5029 58 tf
PA5403 58 tf
PA5437 58 tf
PA0116 422 tf
PA0155 422 tf
PA0236 422 tf
PA0475 422 tf
PA0707 422 tf
PA1141 422 tf
PA1197 422 tf
PA1241 422 tf
PA1290 422 tf
PA1312 422 tf
PA1397 422 tf
PA1490 422 tf
PA1853 422 tf
PA2047 422 tf
PA2115 422 tf
PA2802 422 tf
PA3067 422 tf
PA3322 422 tf
PA3622 422 tf
PA3921 422 tf
PA4077 422 tf
PA4547 422 tf
PA4853 422 tf
PA5356 422 tf
PA5382 422 tf
PA5550 422 tf

Warning: PA4077 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2948 6.80e+01 GgAa.AAaaCCTTg
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2949 1.20e+00 aTaGGTcataAtGacTgCaAccAt
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3666 4.80e-04 aaaAagGGgc.cgAct.TagC
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3667 2.60e+01 AATGTAaagcttGCTTgaCaAAGA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4077

PA4077 is enriched for 2 functions in 3 categories.
Enrichment Table (2)
Function System
Predicted transcriptional regulators cog/ cog
sequence-specific DNA binding go/ molecular_function
Module neighborhood information for PA4077

PA4077 has total of 47 gene neighbors in modules 58, 422
Gene neighbors (47)
Gene Common Name Description Module membership
PA0063 PA0063 hypothetical protein (NCBI) 58, 170
PA0123 PA0123 probable transcriptional regulator (NCBI) 58, 541
PA0225 PA0225 probable transcriptional regulator (NCBI) 58, 489
PA0310 PA0310 hypothetical protein (NCBI) 48, 422
PA0350 folA dihydrofolate reductase (NCBI) 58, 351
PA0463 creB two-component response regulator CreB (NCBI) 58, 351
PA0562 PA0562 probable hydrolase (NCBI) 58, 313
PA0574 PA0574 hypothetical protein (NCBI) 58, 138
PA0757 PA0757 probable two-component sensor (NCBI) 58, 283
PA0831 oruR transcriptional regulator OruR (NCBI) 58, 139
PA0874 PA0874 hypothetical protein (NCBI) 58, 101
PA1116 PA1116 hypothetical protein (NCBI) 230, 422
PA1394 PA1394 hypothetical protein (NCBI) 372, 422
PA1395 PA1395 hypothetical protein (NCBI) 372, 422
PA1574 PA1574 hypothetical protein (NCBI) 58, 541
PA1958 PA1958 probable transporter (NCBI) 58, 422
PA2664 fhp flavohemoprotein (NCBI) 58, 110
PA2665 PA2665 anaerobic nitric oxide reductase transcription regulator (NCBI) 58, 373
PA2876 pyrF orotidine 5'-phosphate decarboxylase (NCBI) 247, 422
PA2942 PA2942 probable magnesium chelatase (NCBI) 58, 490
PA2956 PA2956 hypothetical protein (NCBI) 344, 422
PA3050 pyrD dihydroorotate dehydrogenase (NCBI) 111, 422
PA3322 PA3322 hypothetical protein (NCBI) 58, 387
PA3453 PA3453 hypothetical protein (NCBI) 58, 72
PA3466 PA3466 probable ATP-dependent RNA helicase (NCBI) 58, 170
PA3766 PA3766 probable aromatic amino acid transporter (NCBI) 58, 247
PA3898 PA3898 probable transcriptional regulator (NCBI) 58, 187
PA3967 PA3967 hypothetical protein (NCBI) 422, 520
PA4013 PA4013 hypothetical protein (NCBI) 351, 422
PA4014 PA4014 hypothetical protein (NCBI) 58, 351
PA4075 PA4075 hypothetical protein (NCBI) 229, 422
PA4076 PA4076 hypothetical protein (NCBI) 58, 422
PA4077 PA4077 probable transcriptional regulator (NCBI) 58, 422
PA4113 PA4113 sugar efflux transporter (NCBI) 377, 422
PA4233 PA4233 probable major facilitator superfamily (MFS) transporter (NCBI) 299, 422
PA4396 PA4396 probable two-component response regulator (NCBI) 58, 374
PA4436 PA4436 probable transcriptional regulator (NCBI) 58, 340
PA4445 PA4445 hypothetical protein (NCBI) 299, 422
PA4644 PA4644 hypothetical protein (NCBI) 65, 422
PA4988 waaA 3-deoxy-D-manno-octulosonic-acid transferase (NCBI) 266, 422
PA4991 PA4991 hypothetical protein (NCBI) 58, 266
PA5029 PA5029 probable transcriptional regulator (NCBI) 58, 196
PA5034 hemE uroporphyrinogen decarboxylase (NCBI) 335, 422
PA5193 hslO Hsp33-like chaperonin (NCBI) 422, 520
PA5370 PA5370 probable major facilitator superfamily (MFS) transporter (NCBI) 58, 220
PA5403 PA5403 probable transcriptional regulator (NCBI) 58, 99
PA5529 PA5529 probable sodium/proton antiporter (NCBI) 220, 422
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4077
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend