Organism : Pseudomonas aeruginosa | Module List :
PA5015 aceE

pyruvate dehydrogenase (NCBI)

CircVis
Functional Annotations (12)
Function System
Pyruvate dehydrogenase complex, dehydrogenase (E1) component cog/ cog
pyruvate dehydrogenase (acetyl-transferring) activity go/ molecular_function
metabolic process go/ biological_process
Glycolysis / Gluconeogenesis kegg/ kegg pathway
Citrate cycle (TCA cycle) kegg/ kegg pathway
Valine leucine and isoleucine biosynthesis kegg/ kegg pathway
Pyruvate metabolism kegg/ kegg pathway
Butanoate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
aceE tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA5015
(Mouseover regulator name to see its description)

PA5015 is regulated by 43 influences and regulates 0 modules.
Regulators for PA5015 aceE (43)
Regulator Module Operator
PA0123 213 tf
PA0448 213 tf
PA0652 213 tf
PA1184 213 tf
PA1309 213 tf
PA1949 213 tf
PA1998 213 tf
PA2020 213 tf
PA3002 213 tf
PA3571 213 tf
PA3583 213 tf
PA4080 213 tf
PA4238 213 tf
PA4269 213 tf
PA4270 213 tf
PA4581 213 tf
PA4755 213 tf
PA5189 213 tf
PA5253 213 tf
PA5324 213 tf
PA0163 47 tf
PA0279 47 tf
PA0393 47 tf
PA0448 47 tf
PA0512 47 tf
PA0576 47 tf
PA0652 47 tf
PA1099 47 tf
PA1998 47 tf
PA2020 47 tf
PA2050 47 tf
PA2126 47 tf
PA3002 47 tf
PA3225 47 tf
PA3879 47 tf
PA4070 47 tf
PA4269 47 tf
PA4270 47 tf
PA4493 47 tf
PA4755 47 tf
PA5125 47 tf
PA5166 47 tf
PA5253 47 tf

Warning: PA5015 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2926 2.20e+01 tagctaTttT.CcT
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2927 1.40e+03 tttcGGatgtT
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3254 1.70e+03 tAgcaAaaCtActA
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3255 4.50e+03 GgCTgtTT.T
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA5015

PA5015 is enriched for 12 functions in 3 categories.
Enrichment Table (12)
Function System
Pyruvate dehydrogenase complex, dehydrogenase (E1) component cog/ cog
pyruvate dehydrogenase (acetyl-transferring) activity go/ molecular_function
metabolic process go/ biological_process
Glycolysis / Gluconeogenesis kegg/ kegg pathway
Citrate cycle (TCA cycle) kegg/ kegg pathway
Valine leucine and isoleucine biosynthesis kegg/ kegg pathway
Pyruvate metabolism kegg/ kegg pathway
Butanoate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
aceE tigr/ tigrfam
Module neighborhood information for PA5015

PA5015 has total of 23 gene neighbors in modules 47, 213
Gene neighbors (23)
Gene Common Name Description Module membership
PA0401 pyrC dihydroorotase (NCBI) 47, 225
PA0402 pyrB aspartate carbamoyltransferase catalytic subunit (NCBI) 47, 225
PA0403 pyrR pyrimidine regulatory protein PyrR (NCBI) 47, 167
PA0426 mexB Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexB (NCBI) 47, 509
PA0427 oprM Major intrinsic multiple antibiotic resistance efflux outer membrane protein OprM precursor (NCBI) 47, 509
PA1155 nrdB ribonucleotide-diphosphate reductase beta subunit (NCBI) 179, 213
PA1156 nrdA ribonucleotide-diphosphate reductase alpha subunit (NCBI) 179, 213
PA1561 aer aerotaxis receptor Aer (NCBI) 47, 167
PA1583 sdhA succinate dehydrogenase (NCBI) 213, 467
PA1584 sdhB succinate dehydrogenase catalytic subunit (NCBI) 213, 467
PA1585 sucA 2-oxoglutarate dehydrogenase (E1 subunit) (NCBI) 213, 244
PA1586 sucB dihydrolipoamide acetyltransferase (NCBI) 213, 244
PA1587 lpdG dihydrolipoamide dehydrogenase (NCBI) 213, 244
PA1588 sucC succinyl-CoA synthetase subunit beta (NCBI) 213, 467
PA1589 sucD succinyl-CoA synthetase alpha subunit (NCBI) 213, 467
PA2612 serS seryl-tRNA synthetase (NCBI) 47, 121
PA5015 aceE pyruvate dehydrogenase (NCBI) 47, 213
PA5016 aceF dihydrolipoamide acetyltransferase (NCBI) 47, 213
PA5119 glnA glutamine synthetase (NCBI) 47, 167
PA5242 ppk polyphosphate kinase (NCBI) 47, 167
PA5243 hemB delta-aminolevulinic acid dehydratase (NCBI) 47, 167
PA5435 PA5435 oxaloacetate decarboxylase (NCBI) 47, 179
PA5436 PA5436 acetyl-CoA carboxylase (NCBI) 47, 179
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA5015
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend