Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0296 cycA

Cytochrome c2 (NCBI)

CircVis
Functional Annotations (5)
Function System
Cytochrome c2 cog/ cog
iron ion binding go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
heme binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0296
(Mouseover regulator name to see its description)

RSP_0296 is regulated by 19 influences and regulates 0 modules.
Regulators for RSP_0296 cycA (19)
Regulator Module Operator
RSP_0068 21 tf
RSP_0386 21 tf
RSP_0547 21 tf
RSP_0611 21 tf
RSP_0755 21 tf
RSP_1032 21 tf
RSP_1231 21 tf
RSP_1518 21 tf
RSP_1660 21 tf
RSP_2606 21 tf
RSP_3238 21 tf
RSP_0547 45 tf
RSP_0601 45 tf
RSP_1518 45 tf
RSP_2346 45 tf
RSP_2888 45 tf
RSP_2963 45 tf
RSP_3238 45 tf
RSP_3341 45 tf

Warning: RSP_0296 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7762 1.10e+00 a.AcTG.cAA
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7763 5.20e+02 TTctTcGA
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7810 2.80e+01 aaGcGCGGcGAAAtGcGgcA
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7811 1.70e+02 CcTcCTgTcCtGcCGggaAgcCta
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0296

RSP_0296 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Cytochrome c2 cog/ cog
iron ion binding go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
heme binding go/ molecular_function
Module neighborhood information for RSP_0296

RSP_0296 has total of 37 gene neighbors in modules 21, 45
Gene neighbors (37)
Gene Common Name Description Module membership
RSP_0254 dxsA deoxyxylulose-5-phosphate synthase (NCBI) 45, 144
RSP_0269 tspO Tryptophan rich sensory protein (NCBI) 45, 144
RSP_0273 bchI Magnesium-chelatase, ChlI subunit (NCBI) 45, 260
RSP_0274 bchD Magnesium-chelatase, subunit D (NCBI) 45, 260
RSP_0296 cycA Cytochrome c2 (NCBI) 21, 45
RSP_0315 pucC Light-harvesting 1 (B870) complex assembly (NCBI) 45, 163
RSP_0474 cycP Cytochrome c' (NCBI) 21, 264
RSP_0820 RSP_0820 Putative cytochrome B561 (NCBI) 45, 275
RSP_1394 fbcC Cytochrome c1 precursor (NCBI) 21, 118
RSP_1395 fbcB Cytochrome b (NCBI) 21, 118
RSP_1396 fbcF Ubiquinol-cytochrome C reductase, iron-sulfur protein (NCBI) 21, 118
RSP_1556 puc2B light-harvesting complex, beta subunit (NCBI) 21, 230
RSP_1762 RSP_1762 hypothetical protein (NCBI) 21, 144
RSP_1836 apt adenine phosphoribosyltransferase (NCBI) 45, 382
RSP_1837 RSP_1837 Purine nucleoside phosphorylase, probable 5'-methylthioadenosine phosphorylase (NCBI) 45, 382
RSP_2046 RSP_2046 hypothetical protein (NCBI) 21, 118
RSP_2086 RSP_2086 Putative Antibiotic biosynthesis monooxygenase (NCBI) 45, 264
RSP_2087 RSP_2087 hypothetical protein (NCBI) 45, 139
RSP_2386 RSP_2386 translation initiation factor eIF-2B, alpha subunit (NCBI) 45, 382
RSP_2687 fbcQ cytochrome b-c1 subunit IV (NCBI) 21, 45
RSP_2688 soxD putative sarcosine oxidase delta subunit protein (NCBI) 21, 172
RSP_2984 hemA 5-AMINOLEVULINIC ACID SYNTHASE 1 (NCBI) 45, 260
RSP_3070 RSP_3070 hypothetical protein (NCBI) 45, 144
RSP_3361 RSP_3361 Putative restriction endonuclease or methylase (NCBI) 21, 45
RSP_3588 RSP_3588 hypothetical protein (NCBI) 45, 118
RSP_3640 RSP_3640 hypothetical protein (NCBI) 21, 305
RSP_3641 RSP_3641 possible pfkB family carbohydrate kinase (NCBI) 21, 305
RSP_3642 exsB putative ExsB (NCBI) 21, 305
RSP_3643 RSP_3643 hypothetical protein (NCBI) 21, 305
RSP_3706 RSP_3706 hypothetical protein (NCBI) 45, 144
RSP_3750 RSP_3750 hypothetical protein (NCBI) 21, 358
RSP_3751 RSP_3751 hypothetical protein (NCBI) 21, 358
RSP_3752 RSP_3752 hypothetical protein (NCBI) 21, 358
RSP_3754 RSP_3754 hypothetical protein (NCBI) 21, 100
RSP_3755 RSP_3755 hypothetical protein (NCBI) 21, 358
RSP_3756 RSP_3756 hypothetical protein (NCBI) 21, 358
RSP_3757 RSP_3757 hypothetical protein (NCBI) 21, 358
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0296
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend