Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0601 rpoH2

sigma factor RpoH2 (Sigma-32 group (NCBI)

CircVis
Functional Annotations (7)
Function System
DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32) cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
transcription initiation, DNA-dependent go/ biological_process
regulation of transcription, DNA-dependent go/ biological_process
sigma factor activity go/ molecular_function
RNA polymerase kegg/ kegg pathway
sigma70-ECF tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0601
(Mouseover regulator name to see its description)

RSP_0601 is regulated by 22 influences and regulates 31 modules.
Regulators for RSP_0601 rpoH2 (22)
Regulator Module Operator
RSP_0018 58 tf
RSP_0068 58 tf
RSP_0547 58 tf
RSP_0601 58 tf
RSP_0611 58 tf
RSP_1274 58 tf
RSP_1776 58 tf
RSP_1892 58 tf
RSP_2346 58 tf
RSP_2850 58 tf
RSP_2888 58 tf
RSP_3179 58 tf
RSP_3341 58 tf
RSP_0443 161 tf
RSP_0601 161 tf
RSP_1032 161 tf
RSP_1092 161 tf
RSP_2130 161 tf
RSP_2351 161 tf
RSP_2410 161 tf
RSP_2533 161 tf
RSP_3179 161 tf

Warning: RSP_0601 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7836 6.20e-04 tGtTTTT
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7837 8.10e+01 TTCcAGAcCgAAagtTtAC
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8042 1.60e-07 AgattT.TtgagTcATatcAtAt
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8043 3.90e+00 cTgaTA.Gg.ataA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0601

RSP_0601 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32) cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
transcription initiation, DNA-dependent go/ biological_process
regulation of transcription, DNA-dependent go/ biological_process
sigma factor activity go/ molecular_function
RNA polymerase kegg/ kegg pathway
sigma70-ECF tigr/ tigrfam
Module neighborhood information for RSP_0601

RSP_0601 has total of 54 gene neighbors in modules 58, 161
Gene neighbors (54)
Gene Common Name Description Module membership
RSP_0124 RSP_0124 hypothetical protein (NCBI) 161, 309
RSP_0165 RSP_0165 hypothetical protein (NCBI) 58, 345
RSP_0244 RSP_0244 Predicted glutamine amidotransferases (NCBI) 46, 161
RSP_0418 RSP_0418 hypothetical protein (NCBI) 58, 234
RSP_0442 RSP_0442 putative aminotransferase (NCBI) 159, 161
RSP_0443 RSP_0443 Rrf2 family transcriptional regulator (NCBI) 161, 289
RSP_0525 RSP_0525 hypothetical protein (NCBI) 24, 161
RSP_0601 rpoH2 sigma factor RpoH2 (Sigma-32 group (NCBI) 58, 161
RSP_0611 RSP_0611 transcriptional regulator, ArsR family (NCBI) 58, 274
RSP_0705 RSP_0705 Cytochrome cy (NCBI) 58, 271
RSP_0850 RSP_0850 conserved hypothetical transmembrane protein (NCBI) 161, 287
RSP_0899 RSP_0899 AhpC/TSA family protein (NCBI) 58, 142
RSP_1403 RSP_1403 conserved hypoothetical protein (NCBI) 58, 181
RSP_1409 RSP_1409 Beta-Ig-H3/Fasciclin (NCBI) 2, 161
RSP_1545 RSP_1545 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites (NCBI) 161, 289
RSP_1546 bfr Bacterioferritin (NCBI) 161, 232
RSP_1547 RSP_1547 probable bacterioferritin-associated ferredoxin (NCBI) 161, 232
RSP_1692 pduO hypothetical protein (NCBI) 58, 231
RSP_1825 tldD probable modulator of DNA gyrase (NCBI) 161, 186
RSP_1852 RSP_1852 hypothetical protein (NCBI) 46, 161
RSP_1948 RSP_1948 Protein of unknown function, HesB/YadR/YfhF (NCBI) 161, 309
RSP_1949 RSP_1949 hypothetical protein (NCBI) 161, 309
RSP_2082 RSP_2082 putative NADH-ubiquinone oxidoreductase-related protein (NCBI) 58, 372
RSP_2128 purQ phosphoribosylformylglycinamidine synthase I (FGAM) synthase (NCBI) 58, 231
RSP_2161 bcrC Efflux channel (NCBI) 26, 58
RSP_2228 RSP_2228 PAS domain containing protein (NCBI) 58, 353
RSP_2229 RSP_2229 Chemotaxis multidomain, CheB methylesterase/CheR, Methylase (NCBI) 58, 353
RSP_2346 RSP_2346 Cold-shock DNA-binding domain protein (NCBI) 58, 274
RSP_2347 RSP_2347 hypothetical protein (NCBI) 58, 274
RSP_2359 RSP_2359 hypothetical protein (NCBI) 161, 331
RSP_2360 RSP_2360 putative head portal protein, HK97 family (NCBI) 161, 331
RSP_2361 RSP_2361 putative integrase/resolvase recombinase protein phage-related integrase (NCBI) 93, 161
RSP_2388 RSP_2388 hypothetical protein (NCBI) 161, 210
RSP_2538 fabG Short-chain dehydrogenase/reductase (NCBI) 58, 274
RSP_2547 exoY exopolysaccharide production protein exoY (NCBI) 58, 349
RSP_2548 exoF Outer membrane polysaccharide export protein, exoF (NCBI) 58, 349
RSP_2783 lipA lipoic acid synthetase (NCBI) 161, 309
RSP_2961 RSP_2961 Protein containing a CBS domain (NCBI) 58, 274
RSP_2976 RSP_2976 hypothetical protein (NCBI) 58, 372
RSP_3078 RSP_3078 hypothetical protein (NCBI) 161, 232
RSP_3117 RSP_3117 hypothetical protein (NCBI) 161, 331
RSP_3141 RSP_3141 hypothetical protein (NCBI) 161, 280
RSP_3142 RSP_3142 Na+/solute symporter (NCBI) 161, 280
RSP_3162 RSP_3162 hypothetical protein (NCBI) 58, 231
RSP_3163 RSP_3163 Probable oxidoreductase (NCBI) 58, 231
RSP_3164 RSP_3164 ferredoxin like protein (NCBI) 58, 231
RSP_3179 fur probable Ferric-uptake regulator (NCBI) 58, 363
RSP_3567 znuB ABC zinc tranporter, inner membrane subunit ZnuB (NCBI) 161, 331
RSP_3568 znuC ABC zinc transporter, ATPase subunit ZnuC (NCBI) 161, 331
RSP_3569 zur Zinc-uptake regulator, Zur (NCBI) 161, 331
RSP_3571 znuA ABC zinc transporter, periplasmic binding protein ZnuA (NCBI) 161, 331
RSP_3717 RSP_3717 hypothetical protein (NCBI) 17, 58
RSP_3718 RSP_3718 hypothetical protein (NCBI) 17, 58
RSP_3721 RSP_3721 hypothetical protein (NCBI) 58, 274
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0601
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend