Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0643

periplasmic sensor signal transduction histidine kinase (NCBI)

CircVis
Functional Annotations (4)
Function System
Signal transduction histidine kinase, glucose-6-phosphate specific cog/ cog
protein histidine kinase activity go/ molecular_function
ATP binding go/ molecular_function
peptidyl-histidine phosphorylation go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0643
(Mouseover regulator name to see its description)

RSP_0643 is regulated by 22 influences and regulates 0 modules.
Regulators for RSP_0643 (22)
Regulator Module Operator
RSP_0087 118 tf
RSP_0386 118 tf
RSP_0547 118 tf
RSP_0601 118 tf
RSP_0611 118 tf
RSP_1191 118 tf
RSP_1231 118 tf
RSP_1866 118 tf
RSP_2182 118 tf
RSP_2800 118 tf
RSP_2939 118 tf
RSP_3238 118 tf
RSP_0090 92 tf
RSP_0316 92 tf
RSP_0386 92 tf
RSP_0547 92 tf
RSP_0623 92 tf
RSP_0999 92 tf
RSP_1790 92 tf
RSP_1866 92 tf
RSP_3238 92 tf
RSP_3686 92 tf

Warning: RSP_0643 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7904 9.10e-01 T.tcgAGa.AtttaT
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7905 6.20e-02 GgcgCgCttccacCTGcCcg
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7956 3.50e+02 aAcaGGaa
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7957 3.10e+04 CTGaacttCaGcgGc.c.GaA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0643

RSP_0643 is enriched for 4 functions in 3 categories.
Enrichment Table (4)
Function System
Signal transduction histidine kinase, glucose-6-phosphate specific cog/ cog
protein histidine kinase activity go/ molecular_function
ATP binding go/ molecular_function
peptidyl-histidine phosphorylation go/ biological_process
Module neighborhood information for RSP_0643

RSP_0643 has total of 35 gene neighbors in modules 92, 118
Gene neighbors (35)
Gene Common Name Description Module membership
RSP_0162 RSP_0162 hypothetical protein (NCBI) 100, 118
RSP_0184 RSP_0184 putative N-carbamyl-L-amino acid amidohydrolase (NCBI) 92, 102
RSP_0185 RSP_0185 Transcriptional regulator, TetR family (NCBI) 92, 102
RSP_0186 RSP_0186 Transcriptional regulator, TetR family (NCBI) 92, 370
RSP_0382 RSP_0382 poly-beta-hydroxybutyrate polymerase (NCBI) 118, 200
RSP_0582 RSP_0582 possible penicillin binding protein (NCBI) 118, 358
RSP_0643 RSP_0643 periplasmic sensor signal transduction histidine kinase (NCBI) 92, 118
RSP_0787 RSP_0787 hypothetical protein (NCBI) 41, 92
RSP_0788 RSP_0788 hypothetical protein (NCBI) 41, 92
RSP_0872 def1 Formylmethionine deformylase (NCBI) 92, 195
RSP_0940 RSP_0940 ABC transporter, periplasmic substrate binding protein (NCBI) 92, 195
RSP_0941 RSP_0941 ABC transporter, ATPase subunit (NCBI) 92, 176
RSP_0942 RSP_0942 ABC transporter, inner membrane subunit (NCBI) 92, 176
RSP_0971 RSP_0971 Putative membrane protein (NCBI) 43, 118
RSP_0972 RSP_0972 hypothetical protein (NCBI) 43, 118
RSP_0973 RSP_0973 MaoC family protein (NCBI) 67, 118
RSP_1308 RSP_1308 hypothetical protein (NCBI) 92, 102
RSP_1355 RSP_1355 Glycosyl transferase, family 51 (NCBI) 92, 102
RSP_1356 RSP_1356 hypothetical protein (NCBI) 81, 92
RSP_1394 fbcC Cytochrome c1 precursor (NCBI) 21, 118
RSP_1395 fbcB Cytochrome b (NCBI) 21, 118
RSP_1396 fbcF Ubiquinol-cytochrome C reductase, iron-sulfur protein (NCBI) 21, 118
RSP_1567 RSP_1567 Probable sensory transduction histidine kinase (NCBI) 92, 195
RSP_2006 amsA hypothetical protein (NCBI) 92, 329
RSP_2045 RSP_2045 hypothetical protein (NCBI) 100, 118
RSP_2046 RSP_2046 hypothetical protein (NCBI) 21, 118
RSP_2313 RSP_2313 D-isomer specific 2-hydroxyacid dehydrogenase (NCBI) 85, 118
RSP_2830 RSP_2830 hypothetical protein (NCBI) 92, 139
RSP_2831 cobO Probable cob(I)alamin adenosyltransferase (NCBI) 92, 163
RSP_2974 RSP_2974 Putative metalopeptidase (NCBI) 43, 118
RSP_3186 RSP_3186 hypothetical protein (NCBI) 118, 195
RSP_3225 RSP_3225 periplasmic sensor signal transduction histidine kinase (NCBI) 102, 118
RSP_3326 RSP_3326 Low molecular weight phosphotyrosine protein phosphatase (NCBI) 36, 92
RSP_3342 bfr Bacterioferritin (NCBI) 92, 118
RSP_3588 RSP_3588 hypothetical protein (NCBI) 45, 118
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0643
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend