Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0724 mscL

Large-conductance mechanosensitive channel (NCBI)

CircVis
Functional Annotations (5)
Function System
Large-conductance mechanosensitive channel cog/ cog
ion channel activity go/ molecular_function
transport go/ biological_process
membrane go/ cellular_component
mscL tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0724
(Mouseover regulator name to see its description)

RSP_0724 is regulated by 29 influences and regulates 0 modules.
Regulators for RSP_0724 mscL (29)
Regulator Module Operator
RSP_0032 59 tf
RSP_0068 59 tf
RSP_0327 59 tf
RSP_0755 59 tf
RSP_0927 59 tf
RSP_1606 59 tf
RSP_1866 59 tf
RSP_2346 59 tf
RSP_2591 59 tf
RSP_2840 59 tf
RSP_3324 59 tf
RSP_3341 59 tf
RSP_3621 59 tf
RSP_3665 59 tf
RSP_3694 59 tf
RSP_0327 234 tf
RSP_0394 234 tf
RSP_0443 234 tf
RSP_0755 234 tf
RSP_1663 234 tf
RSP_1990 234 tf
RSP_2800 234 tf
RSP_2922 234 tf
RSP_3029 234 tf
RSP_3094 234 tf
RSP_3341 234 tf
RSP_3400 234 tf
RSP_3667 234 tf
RSP_3694 234 tf

Warning: RSP_0724 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7838 2.10e+00 CtccTccctcTcgCtctcaccca
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7839 2.90e+03 TCCTcCtTGCatCc
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8186 3.20e-03 CCGcaACAGGAAgga
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8187 1.30e+03 ccaGtc.gttGAcAaaCcata
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0724

RSP_0724 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Large-conductance mechanosensitive channel cog/ cog
ion channel activity go/ molecular_function
transport go/ biological_process
membrane go/ cellular_component
mscL tigr/ tigrfam
Module neighborhood information for RSP_0724

RSP_0724 has total of 50 gene neighbors in modules 59, 234
Gene neighbors (50)
Gene Common Name Description Module membership
RSP_0163 RSP_0163 putative iron(III) ABC transporter, fused inner membrane subunits (NCBI) 115, 234
RSP_0334 RSP_0334 hypothetical protein (NCBI) 59, 259
RSP_0358 gor probable glutathione reductase (NCBI) 234, 299
RSP_0364 RSP_0364 possible transporter, RarD family, DMT superfamily (NCBI) 38, 234
RSP_0418 RSP_0418 hypothetical protein (NCBI) 58, 234
RSP_0551 RSP_0551 putative thymidylate kinase (NCBI) 234, 340
RSP_0664 RSP_0664 hypothetical protein (NCBI) 59, 344
RSP_0724 mscL Large-conductance mechanosensitive channel (NCBI) 59, 234
RSP_0729 lysA Orn/DAP/Arg decarboxylase, family 2 (NCBI) 54, 234
RSP_0755 nusB putative N utilization substance protein B (NCBI) 234, 240
RSP_0858 RSP_0858 Putative transporter, RhaT family, DMT superfamily (NCBI) 59, 88
RSP_1012 gabD4 succinate-semialdehyde dehdyrogenase (NCBI) 59, 344
RSP_1186 RSP_1186 hypothetical protein (NCBI) 59, 67
RSP_1187 RSP_1187 hypothetical protein (NCBI) 7, 59
RSP_1188 wcaG Nucleotide sugar epimerase/dehydratase (NCBI) 59, 381
RSP_1233 maf putative Maf/YceF/YhdE family protein (NCBI) 87, 234
RSP_1306 RSP_1306 PAS sensor Signal Tranduction Histidine Kinase (NCBI) 4, 234
RSP_1389 aroC Chorismate synthase (NCBI) 181, 234
RSP_1391 thiB ABC thiamine transporter, periplasmic substrate-binding protein (NCBI) 121, 234
RSP_1392 thiP ABC thiamine transporter, inner membrane subunit (NCBI) 233, 234
RSP_1452 RSP_1452 ABC peptide/nickel/opine transporter, inner membrane subunit (NCBI) 17, 59
RSP_1462 lpxK Putative Tetraacyldisaccharide-1-P 4'-kinase (NCBI) 84, 234
RSP_1472 RSP_1472 hypothetical protein (NCBI) 113, 234
RSP_1505 RSP_1505 Putative Metallo-phosphoesterase (NCBI) 59, 344
RSP_1507 RSP_1507 Aldehyde dehydrogenase (NCBI) 59, 344
RSP_1800 RSP_1800 hypothetical protein (NCBI) 234, 293
RSP_1928 dnaB Replicative DNA helicase, DnaB (NCBI) 76, 234
RSP_2155 hisD Histidinol dehydrogenase (NCBI) 17, 59
RSP_2158 RSP_2158 ABC transporter, periplasmic solute-binding protein (NCBI) 57, 59
RSP_2159 RSP_2159 ABC transporter, ATPase subunit (NCBI) 59, 299
RSP_2205 RSP_2205 hypothetical protein (NCBI) 67, 234
RSP_2206 RSP_2206 hypothetical protein (NCBI) 67, 234
RSP_2306 RSP_2306 putative HAD superfamily protein (NCBI) 234, 279
RSP_2334 pfkB carbohydrate kinase, PfkB (NCBI) 59, 344
RSP_2407 RSP_2407 periplasmic sensor hybrid histidine kinase (NCBI) 234, 322
RSP_2492 RSP_2492 Possible transporter, RhaT family, DMT superfamily (NCBI) 54, 234
RSP_2496 RSP_2496 hypothetical protein (NCBI) 47, 59
RSP_2577 cycI isocytochrome c2 (NCBI) 26, 59
RSP_2800 acoR Putative AcoR, Transcriptional activator of acetoin/glycerol metabolism (NCBI) 234, 337
RSP_2817 aroK Putative shikimate kinase (NCBI) 26, 234
RSP_2818 aroB Putative 3-dehydroquinate synthase (NCBI) 84, 234
RSP_2844 RSP_2844 Putative GTP-binding protein (NCBI) 59, 114
RSP_2896 RSP_2896 Multiple antibiotic transporter (NCBI) 15, 234
RSP_3401 RSP_3401 diguanylate cyclase (NCBI) 17, 59
RSP_3508 RSP_3508 predicted acetamidase/formamidase (NCBI) 59, 344
RSP_3538 RSP_3538 hypothetical protein (NCBI) 59, 344
RSP_3539 RSP_3539 Hemolysin-type calcium-binding region, RTX (NCBI) 59, 344
RSP_3540 RSP_3540 Putative adhesin or RTX toxin (NCBI) 59, 344
RSP_3833 fadB enoyl CoA hydratase (NCBI) 59, 278
RSP_3834 RSP_3834 hypothetical protein (NCBI) 59, 274
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0724
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend