Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1219 grpE

putative chaperone protein GrpE (heat shock protein) (NCBI)

CircVis
Functional Annotations (5)
Function System
Molecular chaperone GrpE (heat shock protein) cog/ cog
adenyl-nucleotide exchange factor activity go/ molecular_function
protein folding go/ biological_process
protein homodimerization activity go/ molecular_function
chaperone binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1219
(Mouseover regulator name to see its description)

RSP_1219 is regulated by 27 influences and regulates 0 modules.
Regulators for RSP_1219 grpE (27)
Regulator Module Operator
RSP_0591 7 tf
RSP_0623 7 tf
RSP_1014 7 tf
RSP_1225 7 tf
RSP_1272 7 tf
RSP_1590 7 tf
RSP_1606 7 tf
RSP_2171 7 tf
RSP_2236 7 tf
RSP_2838 7 tf
RSP_2840 7 tf
RSP_2850 7 tf
RSP_2950 7 tf
RSP_2965 7 tf
RSP_3203 7 tf
RSP_3680 7 tf
RSP_0122 187 tf
RSP_0728 187 tf
RSP_1225 187 tf
RSP_1712 187 tf
RSP_1866 187 tf
RSP_2324 187 tf
RSP_2533 187 tf
RSP_2950 187 tf
RSP_2965 187 tf
RSP_3433 187 tf
RSP_3748 187 tf

Warning: RSP_1219 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7736 2.40e+01 TTCCAaGcGactGCCgACTTTa
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7737 4.30e+00 gCaTGgA.AAgcTcatcgC
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8094 1.80e+02 GCgAAaGt
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8095 2.80e+01 GtCaGcagctTcgAAggcaGCAG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1219

RSP_1219 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Molecular chaperone GrpE (heat shock protein) cog/ cog
adenyl-nucleotide exchange factor activity go/ molecular_function
protein folding go/ biological_process
protein homodimerization activity go/ molecular_function
chaperone binding go/ molecular_function
Module neighborhood information for RSP_1219

RSP_1219 has total of 40 gene neighbors in modules 7, 187
Gene neighbors (40)
Gene Common Name Description Module membership
RSP_0586 RSP_0586 probable nicotinate-nucleotide adenylyltransferase (NCBI) 7, 215
RSP_0650 RSP_0650 Probable transglycosylase (NCBI) 7, 33
RSP_0711 YbaB hypothetical protein (NCBI) 173, 187
RSP_0736 mobC MobC protein (NCBI) 12, 187
RSP_0737 mobB Molybdopterin guanine dinucleotide synthesis (NCBI) 12, 187
RSP_0738 mobA molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI) 12, 187
RSP_0866 RSP_0866 hypothetical protein (NCBI) 7, 357
RSP_0877 RSP_0877 Putative transporter, RhaT family, DMT superfamily (NCBI) 29, 187
RSP_0879 RSP_0879 hypothetical protein (NCBI) 7, 65
RSP_0895 RSP_0895 phosphodiesterase-like (NCBI) 7, 175
RSP_0896 RSP_0896 hypothetical protein (NCBI) 7, 87
RSP_0949 ostB putative trehalose-6-phosphate phosphatase (NCBI) 7, 368
RSP_1005 gshA putative glutathione synthetase (NCBI) 7, 181
RSP_1006 RSP_1006 hypothetical protein (NCBI) 7, 10
RSP_1187 RSP_1187 hypothetical protein (NCBI) 7, 59
RSP_1219 grpE putative chaperone protein GrpE (heat shock protein) (NCBI) 7, 187
RSP_1223 tdcF Putative translation initiation inhibitor, yjgF family / putative Endoribonuclease L-PSP (NCBI) 7, 258
RSP_1224 hemN putative Coproporphyrinogen III oxidase and related Fe-S oxidoreductases (NCBI) 68, 187
RSP_1226 RSP_1226 hypothetical protein (NCBI) 133, 187
RSP_1337 aarF probable ubiquinone biosynthesis protein (NCBI) 7, 108
RSP_1380 RSP_1380 hypothetical protein (NCBI) 7, 175
RSP_1387 RSP_1387 AMP nucleosidase (NCBI) 7, 308
RSP_1468 RSP_1468 Site-specific DNA-methyltransferase (NCBI) 7, 218
RSP_1570 purK Phosphoribosylaminoimidazole carboxylase, ATPase subunit (NCBI) 7, 374
RSP_1775 RSP_1775 hypothetical protein (NCBI) 7, 240
RSP_1866 RSP_1866 Transcriptional regulator, AsnC family (NCBI) 187, 356
RSP_2104 RSP_2104 hypothetical protein (NCBI) 7, 298
RSP_2239 RSP_2239 tRNA/rRNA metyltransferase (NCBI) 42, 187
RSP_2544 RSP_2544 Protein-L-isoaspartate O-methyltransferase (NCBI) 7, 72
RSP_2619 trmU tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (NCBI) 187, 356
RSP_2651 RSP_2651 hypothetical protein (NCBI) 7, 110
RSP_2652 RSP_2652 putative Acetyltransferase (NCBI) 7, 110
RSP_2715 RSP_2715 hypothetical protein (NCBI) 7, 114
RSP_2716 lpxB putative lipid-A-disaccharide synthase (NCBI) 7, 240
RSP_2917 RSP_2917 putative cystathionine gamma-synthase beta-lyase (NCBI) 7, 383
RSP_2950 RSP_2950 Transcriptional regulator, LysR family (NCBI) 7, 263
RSP_3209 cbiQ ABC cobalt transporter, inner membrane subunit, CbiQ (NCBI) 7, 100
RSP_3237 lguL Lactoylglutathione lyase (NCBI) 7, 301
RSP_3564 RSP_3564 Generic methyltransferase (NCBI) 7, 215
RSP_3729 RSP_3729 hypothetical protein (NCBI) 187, 354
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_1219
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend