Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2917

putative cystathionine gamma-synthase beta-lyase (NCBI)

CircVis
Functional Annotations (9)
Function System
Cystathionine beta-lyases/cystathionine gamma-synthases cog/ cog
catalytic activity go/ molecular_function
cellular amino acid metabolic process go/ biological_process
pyridoxal phosphate binding go/ molecular_function
Cysteine and methionine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Sulfur metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2917
(Mouseover regulator name to see its description)

RSP_2917 is regulated by 30 influences and regulates 0 modules.
Regulators for RSP_2917 (30)
Regulator Module Operator
RSP_0591 7 tf
RSP_0623 7 tf
RSP_1014 7 tf
RSP_1225 7 tf
RSP_1272 7 tf
RSP_1590 7 tf
RSP_1606 7 tf
RSP_2171 7 tf
RSP_2236 7 tf
RSP_2838 7 tf
RSP_2840 7 tf
RSP_2850 7 tf
RSP_2950 7 tf
RSP_2965 7 tf
RSP_3203 7 tf
RSP_3680 7 tf
RSP_0415 383 tf
RSP_0623 383 tf
RSP_0698 383 tf
RSP_1225 383 tf
RSP_1712 383 tf
RSP_1776 383 tf
RSP_2026 383 tf
RSP_2425 383 tf
RSP_2533 383 tf
RSP_2801 383 tf
RSP_2838 383 tf
RSP_2850 383 tf
RSP_2950 383 tf
RSP_3298 383 tf

Warning: RSP_2917 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7736 2.40e+01 TTCCAaGcGactGCCgACTTTa
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7737 4.30e+00 gCaTGgA.AAgcTcatcgC
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8456 2.20e+01 agGcgcCctTctTtttgG
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8457 2.00e+03 ttCttGgaAA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2917

RSP_2917 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Cystathionine beta-lyases/cystathionine gamma-synthases cog/ cog
catalytic activity go/ molecular_function
cellular amino acid metabolic process go/ biological_process
pyridoxal phosphate binding go/ molecular_function
Cysteine and methionine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Sulfur metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
Module neighborhood information for RSP_2917

RSP_2917 has total of 53 gene neighbors in modules 7, 383
Gene neighbors (53)
Gene Common Name Description Module membership
RSP_0313 RSP_0313 hypothetical protein (NCBI) 115, 383
RSP_0586 RSP_0586 probable nicotinate-nucleotide adenylyltransferase (NCBI) 7, 215
RSP_0650 RSP_0650 Probable transglycosylase (NCBI) 7, 33
RSP_0818 carB carbamoyl phosphate synthase large subunit (RefSeq) 209, 383
RSP_0866 RSP_0866 hypothetical protein (NCBI) 7, 357
RSP_0879 RSP_0879 hypothetical protein (NCBI) 7, 65
RSP_0895 RSP_0895 phosphodiesterase-like (NCBI) 7, 175
RSP_0896 RSP_0896 hypothetical protein (NCBI) 7, 87
RSP_0949 ostB putative trehalose-6-phosphate phosphatase (NCBI) 7, 368
RSP_1005 gshA putative glutathione synthetase (NCBI) 7, 181
RSP_1006 RSP_1006 hypothetical protein (NCBI) 7, 10
RSP_1108 TruB tRNA pseudouridine synthase B (NCBI) 209, 383
RSP_1187 RSP_1187 hypothetical protein (NCBI) 7, 59
RSP_1219 grpE putative chaperone protein GrpE (heat shock protein) (NCBI) 7, 187
RSP_1223 tdcF Putative translation initiation inhibitor, yjgF family / putative Endoribonuclease L-PSP (NCBI) 7, 258
RSP_1288 RSP_1288 ABC branched chain amino acid family transporter, ATPase subunit (NCBI) 263, 383
RSP_1290 RSP_1290 ABC branched chain amino acid family transporter, inner membrane subunit (NCBI) 263, 383
RSP_1337 aarF probable ubiquinone biosynthesis protein (NCBI) 7, 108
RSP_1380 RSP_1380 hypothetical protein (NCBI) 7, 175
RSP_1387 RSP_1387 AMP nucleosidase (NCBI) 7, 308
RSP_1468 RSP_1468 Site-specific DNA-methyltransferase (NCBI) 7, 218
RSP_1570 purK Phosphoribosylaminoimidazole carboxylase, ATPase subunit (NCBI) 7, 374
RSP_1670 spoT/relA RelA/SpoT family protein (NCBI) 130, 383
RSP_1675 rnc Ribonuclease III (NCBI) 263, 383
RSP_1677 RSP_1677 hypothetical protein (NCBI) 221, 383
RSP_1712 rpoC RNA polymerase I subunit A, (NCBI) 104, 383
RSP_1775 RSP_1775 hypothetical protein (NCBI) 7, 240
RSP_1950 RSP_1950 hypothetical protein (NCBI) 213, 383
RSP_2097 RSP_2097 Putative FtsL (NCBI) 374, 383
RSP_2099 murE UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase (NCBI) 374, 383
RSP_2100 murF UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase (NCBI) 374, 383
RSP_2101 mraY Phospho-N-acetylmuramoyl-pentapeptidetransferase (NCBI) 374, 383
RSP_2104 RSP_2104 hypothetical protein (NCBI) 7, 298
RSP_2170 RSP_2170 Phosphate transporter, Pit family (NCBI) 263, 383
RSP_2327 RSP_2327 hypothetical protein (NCBI) 356, 383
RSP_2403 RSP_2403 ABC cobalamin/Fe3+-siderophore transporter, periplasmic substrate-binding subunit (NCBI) 167, 383
RSP_2404 RSP_2404 ABC cobalamin/Fe3+-siderophore transporter, inner membrane subunit (NCBI) 167, 383
RSP_2405 RSP_2405 ABC cobalamin/Fe3+-siderophore transporter, ATPase subunit (NCBI) 167, 383
RSP_2406 RSP_2406 hypothetical protein (NCBI) 167, 383
RSP_2544 RSP_2544 Protein-L-isoaspartate O-methyltransferase (NCBI) 7, 72
RSP_2612 fabH 3-oxoacyl-(acyl-carrier-protein) synthase III (NCBI) 263, 383
RSP_2648 RSP_2648 hypothetical protein (NCBI) 209, 383
RSP_2651 RSP_2651 hypothetical protein (NCBI) 7, 110
RSP_2652 RSP_2652 putative Acetyltransferase (NCBI) 7, 110
RSP_2715 RSP_2715 hypothetical protein (NCBI) 7, 114
RSP_2716 lpxB putative lipid-A-disaccharide synthase (NCBI) 7, 240
RSP_2917 RSP_2917 putative cystathionine gamma-synthase beta-lyase (NCBI) 7, 383
RSP_2950 RSP_2950 Transcriptional regulator, LysR family (NCBI) 7, 263
RSP_3209 cbiQ ABC cobalt transporter, inner membrane subunit, CbiQ (NCBI) 7, 100
RSP_3237 lguL Lactoylglutathione lyase (NCBI) 7, 301
RSP_3564 RSP_3564 Generic methyltransferase (NCBI) 7, 215
RSP_3712 RSP_3712 Multisubunit Na+/H+ antiporter MnhE subunit (NCBI) 311, 383
RSP_3713 RSP_3713 pH adaptation potassium efflux system, phaD subunit (NCBI) 311, 383
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2917
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend