Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1346

DNA gyrase, subunit B (NCBI)

CircVis
Functional Annotations (7)
Function System
Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit cog/ cog
DNA topoisomerase (ATP-hydrolyzing) activity go/ molecular_function
ATP binding go/ molecular_function
chromosome go/ cellular_component
DNA topological change go/ biological_process
DNA modification go/ biological_process
gyrB tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1346
(Mouseover regulator name to see its description)

RSP_1346 is regulated by 26 influences and regulates 0 modules.
Regulators for RSP_1346 (26)
Regulator Module Operator
RSP_0122 372 tf
RSP_0386 372 tf
RSP_0601 372 tf
RSP_0611 372 tf
RSP_0755 372 tf
RSP_0774 372 tf
RSP_1225 372 tf
RSP_1590 372 tf
RSP_1663 372 tf
RSP_1776 372 tf
RSP_2494 372 tf
RSP_2533 372 tf
RSP_2606 372 tf
RSP_3179 372 tf
RSP_0394 192 tf
RSP_0443 192 tf
RSP_0611 192 tf
RSP_0728 192 tf
RSP_1231 192 tf
RSP_1606 192 tf
RSP_1704 192 tf
RSP_1776 192 tf
RSP_2494 192 tf
RSP_2850 192 tf
RSP_2888 192 tf
RSP_2922 192 tf

Warning: RSP_1346 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8104 3.80e-02 CGGaAAaag
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8105 9.40e+00 TagAAgtCGcCCccaAaGaca
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8438 1.40e-05 CCTTgttcTttgcggcCgccttt
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8439 1.60e+03 aAGcagCagaac.aT.tcGGT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1346

RSP_1346 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit cog/ cog
DNA topoisomerase (ATP-hydrolyzing) activity go/ molecular_function
ATP binding go/ molecular_function
chromosome go/ cellular_component
DNA topological change go/ biological_process
DNA modification go/ biological_process
gyrB tigr/ tigrfam
Module neighborhood information for RSP_1346

RSP_1346 has total of 53 gene neighbors in modules 192, 372
Gene neighbors (53)
Gene Common Name Description Module membership
RSP_0193 RSP_0193 hypothetical protein (NCBI) 87, 372
RSP_0195 RSP_0195 NADH-ubiquinone oxidoreductase 17.2 kD subunit (NCBI) 87, 372
RSP_0247 RSP_0247 aminomethyltransferase related to GcvT (NCBI) 317, 372
RSP_0359 cbbI Ribose-5-phosphate isomerase (NCBI) 127, 372
RSP_0366 RSP_0366 Adenylosuccinate synthetase (NCBI) 192, 258
RSP_0553 ruvB Holiday junction DNA helicase RuvB (NCBI) 299, 372
RSP_0633 RSP_0633 UDP-N-acetylglucosamine enolpyruvyl transferase (NCBI) 192, 349
RSP_0687 RSP_0687 Predicted hydrolase (haloacid dehalogenase (HAD) superfamily) (NCBI) 192, 209
RSP_0725 RSP_0725 Thioredoxin, thioldisulfide interchange protein (NCBI) 51, 192
RSP_0744 RSP_0744 DNA-3-methyladenine glycosylase I (NCBI) 192, 349
RSP_0764 RSP_0764 Multi Antimicrobial Extrusion (MATE) family efflux pump NorM (NCBI) 192, 349
RSP_0832 RSP_0832 Putative GTP-binding protein (NCBI) 166, 192
RSP_0882 DapA Dihydrodipicolinate synthetase (NCBI) 182, 372
RSP_0916 pmbA Putative PmbA/TldD protein invoved in modulation of DNA gyrase (NCBI) 192, 218
RSP_0934 gpmI Phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent (NCBI) 192, 299
RSP_1068 argB acetylglutamate kinase (NCBI) 109, 192
RSP_1131 dapD 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase (NCBI) 25, 192
RSP_1150 bacA Bacitracin Resistance Protein (NCBI) 192, 349
RSP_1151 RSP_1151 NADH-ubiquinone oxidoreductase (NCBI) 192, 349
RSP_1152 rnd Ribonuclease D (NCBI) 192, 356
RSP_1153 GutQ Sugar phosphate Isomerase (NCBI) 109, 192
RSP_1200 RSP_1200 putative membrane protein (NCBI) 152, 372
RSP_1221 rph ribonuclease PH (NCBI) 192, 258
RSP_1222 ham1 putative Ham1p_like protein, Ham1 family (NCBI) 192, 258
RSP_1343 RSP_1343 DNA polymerase III, beta chain (NCBI) 25, 372
RSP_1346 RSP_1346 DNA gyrase, subunit B (NCBI) 192, 372
RSP_1376 asd putative aspartate-semialdehyde dehydrogenase (NCBI) 166, 192
RSP_1390 RSP_1390 Putative transporter, DMT superfamily (NCBI) 192, 331
RSP_1490 RSP_1490 Putative ATP-dependent protease La, LON (NCBI) 231, 372
RSP_1511 RSP_1511 hypothetical protein (NCBI) 215, 372
RSP_1562 RSP_1562 fumarylacetoacetate hydrolase family protein (NCBI) 294, 372
RSP_1580 lgt Prolipoprotein diacylglyceryl transferase (NCBI) 192, 339
RSP_1776 greA Transcription elongation factor greA (NCBI) 240, 372
RSP_1795 serS Probable Seryl-tRNA synthetase (NCBI) 109, 192
RSP_1831 thrC Threonine synthase (NCBI) 109, 192
RSP_1833 RSP_1833 putative ribosomal-protein-alanine acetyltransferase (NCBI) 326, 372
RSP_1875 RSP_1875 hypothetical protein (NCBI) 345, 372
RSP_1947 cbbJ Triosephosphate isomerase (NCBI) 278, 372
RSP_1969 purM Phosphoribosylformylglycinamidine cyclo-ligase (NCBI) 258, 372
RSP_2009 argF Aspartate/ornithine carbamoyltransferase (NCBI) 192, 372
RSP_2038 RSP_2038 possible acetyltransferase (NCBI) 213, 372
RSP_2082 RSP_2082 putative NADH-ubiquinone oxidoreductase-related protein (NCBI) 58, 372
RSP_2083 uvrB Putative Excinuclease subunit B (NCBI) 152, 372
RSP_2519 RSP_2519 hypothetical protein (NCBI) 269, 372
RSP_2533 RSP_2533 Putative transcriptional regulator (NCBI) 361, 372
RSP_2542 RSP_2542 ATPase (NCBI) 130, 372
RSP_2647 RSP_2647 Predicted SAM-dependent methyltransferases (NCBI) 192, 372
RSP_2943 argS Arginyl-tRNA synthetase, class I (NCBI) 160, 372
RSP_2968 RSP_2968 Probable dihydrolipoamide dehydrogenase (NCBI) 184, 192
RSP_2976 RSP_2976 hypothetical protein (NCBI) 58, 372
RSP_3261 bioA Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (BioA) (NCBI) 354, 372
RSP_3262 RSP_3262 Putative thioesterase (NCBI) 259, 372
RSP_4315 RSP_4315 tRNA-Met (NCBI) 75, 372
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_1346
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend