Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_1861

probable dihydroneopterin aldolase (NCBI)

CircVis
Functional Annotations (6)
Function System
Dihydroneopterin aldolase cog/ cog
dihydroneopterin aldolase activity go/ molecular_function
folic acid-containing compound metabolic process go/ biological_process
Folate biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
folB_dom tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_1861
(Mouseover regulator name to see its description)

RSP_1861 is regulated by 17 influences and regulates 0 modules.
Regulators for RSP_1861 (17)
Regulator Module Operator
RSP_0623 326 tf
RSP_0722 326 tf
RSP_0755 326 tf
RSP_1712 326 tf
RSP_2200 326 tf
RSP_2533 326 tf
RSP_2838 326 tf
RSP_2965 326 tf
RSP_0014 215 tf
RSP_0547 215 tf
RSP_0611 215 tf
RSP_0623 215 tf
RSP_1191 215 tf
RSP_1518 215 tf
RSP_1776 215 tf
RSP_3385 215 tf
RSP_3680 215 tf

Warning: RSP_1861 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8150 1.90e+01 ctGaTcgacATCGACgAgctcaA
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8151 1.20e+04 aaCtGACGGTCGa
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8360 1.70e+04 gAgC.TGA
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8361 1.90e+04 GAAcTcCAgC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_1861

RSP_1861 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Dihydroneopterin aldolase cog/ cog
dihydroneopterin aldolase activity go/ molecular_function
folic acid-containing compound metabolic process go/ biological_process
Folate biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
folB_dom tigr/ tigrfam
Module neighborhood information for RSP_1861

RSP_1861 has total of 52 gene neighbors in modules 215, 326
Gene neighbors (52)
Gene Common Name Description Module membership
RSP_0014 RSP_0014 transcriptional regulator, TetR family (NCBI) 91, 215
RSP_0018 RSP_0018 Putative transcriptional regulator, gntR family (NCBI) 285, 326
RSP_0337 RSP_0337 possible phospodiesterase (NCBI) 120, 215
RSP_0338 RSP_0338 hypothetical protein (NCBI) 120, 215
RSP_0363 RSP_0363 hypothetical protein (NCBI) 215, 218
RSP_0450 RSP_0450 hypothetical protein (NCBI) 293, 326
RSP_0586 RSP_0586 probable nicotinate-nucleotide adenylyltransferase (NCBI) 7, 215
RSP_0666 RSP_0666 Probable cell cycle protein (NCBI) 88, 326
RSP_0667 RSP_0667 hypothetical protein (NCBI) 20, 326
RSP_0669 RSP_0669 Probable TolB protein precursor (NCBI) 326, 339
RSP_0670 RSP_0670 Possible TolA protein (NCBI) 277, 326
RSP_0700 RSP_0700 probable glycosyltransferase (NCBI) 233, 326
RSP_0701 RSP_0701 ABC peptide transporter, fused ATPase domains (NCBI) 233, 326
RSP_0782 RSP_0782 Ppx/GppA phosphatase (NCBI) 215, 240
RSP_0784 RSP_0784 putative acetyltransferase, GNAT family (NCBI) 215, 240
RSP_0814 RSP_0814 Acetyltransferase (NCBI) 215, 248
RSP_1004 RSP_1004 hypothetical protein (NCBI) 23, 326
RSP_1094 RSP_1094 hypothetical protein (NCBI) 17, 326
RSP_1143 RSP_1143 4Fe-4S ferredoxin, iron-sulfur cluster binding protein (NCBI) 94, 326
RSP_1189 wcaJ sugar transferase (NCBI) 47, 215
RSP_1190 RSP_1190 hypothetical protein (NCBI) 215, 368
RSP_1191 RSP_1191 transcriptional regulator, MarR family (NCBI) 215, 368
RSP_1192 RSP_1192 None 215, 368
RSP_1214 RSP_1214 putative branched-chain amino acid aminotransferase (NCBI) 215, 381
RSP_1275 RSP_1275 Crp-Fnr family transciptional regulator (NCBI) 215, 340
RSP_1276 RSP_1276 hypothetical protein (NCBI) 215, 368
RSP_1287 RSP_1287 ABC branched chain amino acid family transporter, ATPase subunit (NCBI) 215, 263
RSP_1369 RSP_1369 Short-chain dehydrogenase/reductase SDR (NCBI) 126, 215
RSP_1506 RSP_1506 Pseudouridine synthase, Rlu (NCBI) 322, 326
RSP_1511 RSP_1511 hypothetical protein (NCBI) 215, 372
RSP_1512 RSP_1512 hypothetical protein (NCBI) 121, 326
RSP_1664 RSP_1664 hypothetical protein (NCBI) 326, 339
RSP_1746 RSP_1746 hypothetical protein (NCBI) 326, 361
RSP_1833 RSP_1833 putative ribosomal-protein-alanine acetyltransferase (NCBI) 326, 372
RSP_1861 RSP_1861 probable dihydroneopterin aldolase (NCBI) 215, 326
RSP_1975 RSP_1975 aminopeptidase P (NCBI) 208, 326
RSP_2098 ftsI Cell division protein FtsI/penicillin-binding protein 2 (NCBI) 326, 374
RSP_2148 RSP_2148 Small Conductance Mechanosensitive Ion Channel (NCBI) 326, 339
RSP_2149 RSP_2149 Alanyl-transfer RNA synthetase domain protein (NCBI) 326, 339
RSP_2312 RSP_2312 hypothetical protein (NCBI) 215, 231
RSP_2375 RSP_2375 hypothetical protein (NCBI) 215, 366
RSP_2382 RSP_2382 hypothetical protein (NCBI) 215, 294
RSP_2391 RSP_2391 2'-5' RNA ligase (NCBI) 126, 215
RSP_2495 nrd putative class II ribonucleoside-diphosphate reductase (NCBI) 293, 326
RSP_2634 ccmH Cytochrome c maturation protein, CcmH (NCBI) 215, 368
RSP_2678 RSP_2678 hypothetical protein (NCBI) 215, 340
RSP_2788 RSP_2788 hypothetical protein (NCBI) 36, 215
RSP_2815 xerD Probable integrase/recombinase XerD (NCBI) 84, 326
RSP_3023 RSP_3023 hypothetical protein (NCBI) 215, 303
RSP_3402 RSP_3402 multisensor diguanylate cyclase/phosphodiesterase (NCBI) 17, 215
RSP_3564 RSP_3564 Generic methyltransferase (NCBI) 7, 215
RSP_3680 RSP_3680 transcriptional regulator, TetR family (NCBI) 143, 215
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_1861
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend