Organism : Bacillus cereus ATCC14579 | Module List :
BC2289

3-hydroxyisobutyrate dehydrogenase (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases cog/ cog
phosphogluconate dehydrogenase (decarboxylating) activity go/ molecular_function
pentose-phosphate shunt go/ biological_process
valine metabolic process go/ biological_process
3-hydroxyisobutyrate dehydrogenase activity go/ molecular_function
coenzyme binding go/ molecular_function
Valine leucine and isoleucine degradation kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BC2289
(Mouseover regulator name to see its description)

BC2289 is regulated by 17 influences and regulates 0 modules.
Regulators for BC2289 (17)
Regulator Module Operator
BC0742 362 tf
BC1080 362 tf
BC1115 362 tf
BC2178 362 tf
BC3588 362 tf
BC0057 212 tf
BC0953 212 tf
BC1115 212 tf
BC1253 212 tf
BC2410 212 tf
BC2738 212 tf
BC3025 212 tf
BC3423 212 tf
BC3588 212 tf
BC4661 212 tf
BC4960 212 tf
BC5363 212 tf

Warning: BC2289 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
4340 6.50e-02 CTCCTTc
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4341 3.20e+04 AGGGcG
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4634 3.80e-02 aGGgGG
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4635 6.00e+00 ctgctTT.acAAAaaaacaacc.T
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BC2289

BC2289 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases cog/ cog
phosphogluconate dehydrogenase (decarboxylating) activity go/ molecular_function
pentose-phosphate shunt go/ biological_process
valine metabolic process go/ biological_process
3-hydroxyisobutyrate dehydrogenase activity go/ molecular_function
coenzyme binding go/ molecular_function
Valine leucine and isoleucine degradation kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for BC2289

BC2289 has total of 35 gene neighbors in modules 212, 362
Gene neighbors (35)
Gene Common Name Description Module membership
BC0252 BC0252 4-hydroxyphenylpyruvate dioxygenase (NCBI ptt file) 178, 362
BC0253 BC0253 Fumarylacetoacetase (NCBI ptt file) 178, 362
BC0268 BC0268 Zinc metalloprotease (NCBI ptt file) 212, 424
BC0440 BC0440 surface protein (NCBI ptt file) 291, 362
BC0441 BC0441 Glucokinase (NCBI ptt file) 258, 362
BC0487 BC0487 UDP-N-acetyl-D-mannosamine 6-dehydrogenase (NCBI ptt file) 212, 376
BC0655 BC0655 Universal stress protein family (NCBI ptt file) 212, 454
BC0945 BC0945 DNA integration/recombination/invertion protein (NCBI ptt file) 85, 212
BC1255 BC1255 hypothetical protein (NCBI ptt file) 212, 216
BC1509 BC1509 Stage IV sporulation protein A (NCBI ptt file) 212, 437
BC1801 BC1801 Sensor protein vanS (NCBI ptt file) 212, 279
BC2026 BC2026 Oligopeptide-binding protein oppA (NCBI ptt file) 355, 362
BC2143 BC2143 hypothetical Cytosolic Protein (NCBI ptt file) 34, 362
BC2245 BC2245 6-acetamido-3-oxohexanoate aminotransferase (NCBI ptt file) 362, 495
BC2246 BC2246 putative 3-keto-6-acetamidohexanoate cleavage enzyme (NCBI ptt file) 178, 362
BC2247 BC2247 putative 3-keto-6-acetamidohexanoate cleavage enzyme (NCBI ptt file) 355, 362
BC2248 BC2248 Acetylornithine deacetylase (NCBI ptt file) 362, 495
BC2249 BC2249 Beta-lysine acetyltransferase (NCBI ptt file) 359, 362
BC2288 BC2288 Acyl-CoA dehydrogenase (NCBI ptt file) 212, 279
BC2289 BC2289 3-hydroxyisobutyrate dehydrogenase (NCBI ptt file) 212, 362
BC2290 BC2290 Methylmalonate-semialdehyde dehydrogenase (acylating) (NCBI ptt file) 212, 250
BC2483 BC2483 None 229, 362
BC2486 BC2486 Hydroxymethylglutaryl-CoA lyase (NCBI ptt file) 178, 362
BC2488 BC2488 Propionyl-CoA carboxylase beta chain (NCBI ptt file) 178, 362
BC2489 BC2489 Acetyl-coenzyme A synthetase (NCBI ptt file) 178, 362
BC2855 BC2855 Transcriptional regulator, AraC family (NCBI ptt file) 362, 496
BC3019 BC3019 hypothetical protein (NCBI ptt file) 362, 486
BC3106 BC3106 Small acid-soluble spore protein (NCBI ptt file) 54, 212
BC3158 BC3158 Pyrimidine Reductase (NCBI ptt file) 34, 212
BC3425 BC3425 hypothetical Membrane Associated Protein (NCBI ptt file) 212, 424
BC3610 BC3610 hypothetical protein (NCBI ptt file) 212, 364
BC3634 BC3634 Acetyltransferase (NCBI ptt file) 212, 279
BC4645 BC4645 Acetyl-coenzyme A synthetase (NCBI ptt file) 178, 362
BC5015 BC5015 hypothetical exported repetitive protein (NCBI ptt file) 307, 362
BC5210 BC5210 PTS system, lichenan oligosaccharide-specific IIA component (NCBI ptt file) 212, 446
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BC2289
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend