Organism : Clostridium acetobutylicum | Module List :
CAC3603

Transcriptional regulator, FadR family (NCBI ptt file)

CircVis
Functional Annotations (6)
Function System
Transcriptional regulators cog/ cog
fatty-acyl-CoA binding go/ molecular_function
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
regulation of fatty acid metabolic process go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC3603
(Mouseover regulator name to see its description)

CAC3603 is regulated by 14 influences and regulates 21 modules.
Regulators for CAC3603 (14)
Regulator Module Operator
CAC0254 50 tf
CAC0402 50 tf
CAC0445 50 tf
CAC0474 50 tf
CAC1941 50 tf
CAC3603 50 tf
CAC0240 124 tf
CAC0254 124 tf
CAC0402 124 tf
CAC0474 124 tf
CAC1941 124 tf
CAC2471 124 tf
CAC2486 124 tf
CAC3603 124 tf
Regulated by CAC3603 (21)
Module Residual Genes
10 0.41 22
50 0.19 11
61 0.30 14
63 0.38 20
68 0.35 24
97 0.22 21
120 0.31 15
124 0.22 15
137 0.47 27
148 0.48 16
163 0.33 13
172 0.45 25
181 0.23 15
210 0.30 17
245 0.27 16
295 0.37 22
316 0.35 17
325 0.29 16
327 0.35 18
336 0.38 26
354 0.20 10
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6754 2.40e+00 GAGGtG
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6755 1.30e+03 AGgAGTggaG
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6900 2.70e-01 gaGGtG
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6901 4.10e+01 GcAacGgcgc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC3603

CAC3603 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Transcriptional regulators cog/ cog
fatty-acyl-CoA binding go/ molecular_function
sequence-specific DNA binding transcription factor activity go/ molecular_function
intracellular go/ cellular_component
regulation of transcription, DNA-dependent go/ biological_process
regulation of fatty acid metabolic process go/ biological_process
Module neighborhood information for CAC3603

CAC3603 has total of 21 gene neighbors in modules 50, 124
Gene neighbors (21)
Gene Common Name Description Module membership
CAC0348 CAC0348 Hypothetical protein, CF-21 family (NCBI ptt file) 124, 210
CAC0369 CAC0369 Predicted acetyltransferase (NCBI ptt file) 124, 210
CAC0378 glnQ Glutamine ABC transporter, ATP-binding protein (gene glnQ) (NCBI ptt file) 50, 316
CAC0474 CAC0474 ACT domain containing transcriptional regulators, related to gcvR of E.coli (NCBI ptt file) 50, 124
CAC0785 CAC0785 Hypothetical protein (NCBI ptt file) 50, 311
CAC1219 CAC1219 Hypothetical protein (NCBI ptt file) 50, 181
CAC1417 CAC1417 Hypothetical protein (NCBI ptt file) 124, 337
CAC1971 CAC1971 Hypothetical protein (NCBI ptt file) 50, 120
CAC2192 CAC2192 Lactoylglutathione lyase, YQJC B.subtilis ortholog (NCBI ptt file) 120, 124
CAC2218 cheY Chemotaxis signal receiving protein CheY (NCBI ptt file) 120, 124
CAC2248 CAC2248 Hypothetical protein (NCBI ptt file) 50, 119
CAC2250 CAC2250 UDP-glucose pyrophosphorylase (NCBI ptt file) 50, 124
CAC2256 CAC2256 ABC transporter, ATPase component (NCBI ptt file) 50, 124
CAC2471 CAC2471 Transcriptional regulator, TetR/AcrR family (NCBI ptt file) 124, 257
CAC2667 CAC2667 Possible peptide ABC-type transporter, periplasmic binding component (NCBI ptt file) 50, 163
CAC2717 CAC2717 Ethanolamine ammonia lyase small subunit (NCBI ptt file) 124, 139
CAC2767 CAC2767 Uncharacterized conserved protein (NCBI ptt file) 46, 124
CAC2929 CAC2929 Predicted membrane protein (NCBI ptt file) 120, 124
CAC2958 CAC2958 PTS system enzyme IIBC component (galactitol/fructose specific) (NCBI ptt file) 50, 124
CAC3026 CAC3026 Protein from GDSL (phospholipase B) family of lipolitic enzymes (NCBI ptt file) 124, 210
CAC3603 CAC3603 Transcriptional regulator, FadR family (NCBI ptt file) 50, 124
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC3603
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend