Organism : Campylobacter jejuni | Module List :
Cj0333c fdxA

ferredoxin (NCBI ptt file)

CircVis
Functional Annotations (4)
Function System
CDP-diacylglycerol-serine O-phosphatidyltransferase activity go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj0333c
(Mouseover regulator name to see its description)

Cj0333c is regulated by 11 influences and regulates 0 modules.
Regulators for Cj0333c fdxA (11)
Regulator Module Operator
Cj0287c 13 tf
Cj0400 13 tf
Cj0571 13 tf
Cj0757 13 tf
Cj0123c 91 tf
Cj0287c 91 tf
Cj0400 91 tf
Cj0757 91 tf
Cj1000 91 tf
Cj1273c 91 tf
Cj1533c 91 tf

Warning: Cj0333c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7408 3.30e+01 gCttagAcgcaACggg
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7409 4.30e+02 gcCTgT.Ac.CAag.gccCG
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7564 2.60e+04 TGGTGG
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7565 4.30e+04 CCCAaC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj0333c

Cj0333c is enriched for 4 functions in 2 categories.
Enrichment Table (4)
Function System
CDP-diacylglycerol-serine O-phosphatidyltransferase activity go/ molecular_function
electron transport go/ biological_process
electron carrier activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
Module neighborhood information for Cj0333c

Cj0333c has total of 39 gene neighbors in modules 13, 91
Gene neighbors (39)
Gene Common Name Description Module membership
Cj0012c Cj0012c non-haem iron protein (NCBI ptt file) 82, 91
Cj0019c Cj0019c MCP-domain signal transduction protein (NCBI ptt file) 13, 46
Cj0105 atpA ATP synthase F1 sector alpha subunit (NCBI ptt file) 91, 137
Cj0113 pal peptidoglycan associated lipoprotein (omp18) (NCBI ptt file) 91, 120
Cj0114 Cj0114 putative periplasmic protein (NCBI ptt file) 91, 127
Cj0192c clpP ATP-dependent clp protease proteolytic subunit (NCBI ptt file) 66, 91
Cj0333c fdxA ferredoxin (NCBI ptt file) 13, 91
Cj0350 Cj0350 hypothetical protein Cj0350 (NCBI ptt file) 91, 142
Cj0351 fliN flagellar motor switch protein (NCBI ptt file) 75, 91
Cj0358 Cj0358 putative cytochrome C551 peroxidase (NCBI ptt file) 87, 91
Cj0371 Cj0371 putative flagellar motility protein (NCBI ptt file) 13, 110
Cj0372 Cj0372 hypothetical protein Cj0372 (NCBI ptt file) 13, 18
Cj0373 Cj0373 putative D-2-hydroxyacid dehydrogenase (NCBI ptt file) 13, 81
Cj0374 Cj0374 hypothetical protein Cj0374 (NCBI ptt file) 13, 56
Cj0375 Cj0375 putative lipoprotein (NCBI ptt file) 13, 56
Cj0376 Cj0376 putative periplasmic protein (NCBI ptt file) 13, 17
Cj0377 Cj0377 probable AAA family ATPase (NCBI ptt file) 13, 77
Cj0392c pyk pyruvate kinase (NCBI ptt file) 91, 133
Cj0426 Cj0426 ABC transporter ATP-binding protein (NCBI ptt file) 91, 142
Cj0427 Cj0427 hypothetical protein Cj0427 (NCBI ptt file) 91, 142
Cj0428 Cj0428 hypothetical protein Cj0428 (NCBI ptt file) 91, 102
Cj0453 thiC thiamin biosynthesis protein ThiC (NCBI ptt file) 13, 137
Cj0519 Cj0519 hypothetical protein Cj0519 (NCBI ptt file) 13, 108
Cj0575 ilvH acetolactate synthase small subunit (NCBI ptt file) 13, 110
Cj0893c rpsA 30S ribosomal protein S1 (NCBI ptt file) 91, 119
Cj0918c prsA ribose-phosphate pyrophosphokinase (NCBI ptt file) 91, 95
Cj0919c Cj0919c putative ABC-type amino-acid transporter permease protein (NCBI ptt file) 58, 91
Cj0922c pebC ABC-type amino-acid transporter ATP-binding protein (NCBI ptt file) 91, 142
Cj0982c Cj0982c putative amino-acid transporter periplasmic solute-binding protein (NCBI ptt file) 91, 118
Cj1021c Cj1021c putative periplasmic protein (NCBI ptt file) 61, 91
Cj1074c Cj1074c putative lipoprotein (NCBI ptt file) 13, 114
Cj1153 Cj1153 putative periplasmic cytochrome C (NCBI ptt file) 90, 91
Cj1408 fliL possible flagellar protein (NCBI ptt file) 91, 142
Cj1419c Cj1419c possible methyltransferase (NCBI ptt file) 91, 142
Cj1477c Cj1477c putative hydrolase (NCBI ptt file) 82, 91
Cj1485c Cj1485c putative periplasmic protein (NCBI ptt file) 13, 52
Cj1502c putP sodium/proline symporter (NCBI ptt file) 91, 127
Cj1603 hisF cyclase (NCBI ptt file) 13, 127
Cj1650 Cj1650 hypothetical protein Cj1650 (NCBI ptt file) 86, 91
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj0333c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend