Organism : Campylobacter jejuni | Module List :
Cj1428c fcl

putative fucose synthetase (NCBI ptt file)

CircVis
Functional Annotations (9)
Function System
Nucleoside-diphosphate-sugar epimerases cog/ cog
dTDP-4-dehydrorhamnose reductase activity go/ molecular_function
'de novo' GDP-L-fucose biosynthetic process go/ biological_process
extracellular polysaccharide biosynthetic process go/ biological_process
GDP-L-fucose synthase activity go/ molecular_function
coenzyme binding go/ molecular_function
Fructose and mannose metabolism kegg/ kegg pathway
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for Cj1428c
(Mouseover regulator name to see its description)

Cj1428c is regulated by 2 influences and regulates 0 modules.
Regulators for Cj1428c fcl (2)
Regulator Module Operator
Cj0368c 109 tf
Cj1533c 109 tf

Warning: Cj1428c Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7600 2.60e+04 gC..cTtTAgaAGcT.cTtGg
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7601 2.50e+04 GaAccaTCGG
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7678 2.60e+00 CAAGGA.AA
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7679 2.50e+03 CGATGTTAATCAATGCTTAATCC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for Cj1428c

Cj1428c is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Nucleoside-diphosphate-sugar epimerases cog/ cog
dTDP-4-dehydrorhamnose reductase activity go/ molecular_function
'de novo' GDP-L-fucose biosynthetic process go/ biological_process
extracellular polysaccharide biosynthetic process go/ biological_process
GDP-L-fucose synthase activity go/ molecular_function
coenzyme binding go/ molecular_function
Fructose and mannose metabolism kegg/ kegg pathway
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for Cj1428c

Cj1428c has total of 49 gene neighbors in modules 109, 148
Gene neighbors (49)
Gene Common Name Description Module membership
Cj0035c Cj0035c putative efflux protein (NCBI ptt file) 24, 148
Cj0054c Cj0054c hypothetical protein Cj0054c (NCBI ptt file) 51, 148
Cj0174c Cj0174c putative iron-uptake ABC transport system permease protein (NCBI ptt file) 7, 148
Cj0225 Cj0225 putative acetyltransferase (NCBI ptt file) 104, 109
Cj0276 mreB homolog of E. coli rod shape-determining protein (NCBI ptt file) 76, 148
Cj0303c modA putative molybdate-binding lipoprotein (NCBI ptt file) 123, 148
Cj0349 trpA tryptophan synthase alpha chain (NCBI ptt file) 104, 109
Cj0353c Cj0353c phosphatase (NCBI ptt file) 109, 134
Cj0515 Cj0515 putative periplasmic protein (NCBI ptt file) 99, 148
Cj0544 Cj0544 putative integral memnbrane protein (NCBI ptt file) 148, 161
Cj0561c Cj0561c possible periplasmic protein (NCBI ptt file) 58, 148
Cj0633 Cj0633 putative periplasmic protein (NCBI ptt file) 24, 109
Cj0634 Cj0634 SMF family protein (NCBI ptt file) 106, 109
Cj0635 Cj0635 hypothetical protein Cj0635 (NCBI ptt file) 7, 109
Cj0652 pbpC penicillin-binding protein (NCBI ptt file) 96, 109
Cj0718 dnaE DNA polymerase III, alpha chain (NCBI ptt file) 112, 148
Cj0891c serA D-3-phosphoglycerate dehydrogenase (NCBI ptt file) 63, 148
Cj0985c hipO hippurate hydrolase (NCBI ptt file) 24, 109
Cj1080c Cj1080c hypothetical protein Cj1080c (NCBI ptt file) 118, 148
Cj1081c thiE thiamin-phosphate pyrophosphorylase (NCBI ptt file) 18, 109
Cj1109 aat putative leucyl/phenylalanyl-tRNA--protein transferase (NCBI ptt file) 96, 109
Cj1122c wlaJ putative integral membrane protein (NCBI ptt file) 32, 109
Cj1123c wlaI putative transferase (NCBI ptt file) 96, 109
Cj1124c wlaH putative galactosyltransferase (NCBI ptt file) 109, 118
Cj1125c wlaG putative galactosyltransferase (NCBI ptt file) 106, 109
Cj1127c wlaE putative glycosyltransferase (NCBI ptt file) 109, 125
Cj1174 Cj1174 putative efflux protein (NCBI ptt file) 109, 158
Cj1217c Cj1217c hypothetical protein Cj1217c (NCBI ptt file) 26, 148
Cj1319 Cj1319 putative nucleotide sugar dehydratase (NCBI ptt file) 109, 139
Cj1330 Cj1330 hypothetical protein Cj1330 (NCBI ptt file) 24, 109
Cj1333 Cj1333 hypothetical protein Cj1333 (1318 family) (NCBI ptt file) 63, 109
Cj1374c Cj1374c hypothetical protein Cj1374c (NCBI ptt file) 148, 158
Cj1375 Cj1375 putative efflux protein (NCBI ptt file) 128, 148
Cj1413c Cj1413c possible polysaccharide modification protein (NCBI ptt file) 117, 148
Cj1415c cysC possible adenylylsulfate kinase (NCBI ptt file) 109, 141
Cj1417c Cj1417c hypothetical protein Cj1417c (NCBI ptt file) 24, 109
Cj1418c Cj1418c hypothtical protein Cj1418c (NCBI ptt file) 109, 169
Cj1423c Cj1423c putative sugar-phosphate nucleotidyltransferase (NCBI ptt file) 18, 109
Cj1424c gmhA2 putative phosphoheptose isomerase (NCBI ptt file) 109, 148
Cj1425c Cj1425c putative sugar kinase (NCBI ptt file) 109, 153
Cj1427c Cj1427c putative sugar-nucleotide epimerase/dehydratease (NCBI ptt file) 109, 148
Cj1428c fcl putative fucose synthetase (NCBI ptt file) 109, 148
Cj1429c Cj1429c hypothetical protein Cj1429c (NCBI ptt file) 24, 109
Cj1432c Cj1432c putative sugar transferase (NCBI ptt file) 60, 109
Cj1443c kpsF KpsF protein (NCBI ptt file) 23, 109
Cj1533c Cj1533c putative helix-turn-helix containsing protein (NCBI ptt file) 109, 128
Cj1601 hisA phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (NCBI ptt file) 19, 109
Cj1620c mutY A/G-specific adenine glycosylase (NCBI ptt file) 96, 109
Cj1717c leuC 3-isopropylmalate dehydratase large subunit (NCBI ptt file) 45, 148
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for Cj1428c
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend