Organism : Geobacter sulfurreducens
| Module List :
GSU3286 hemD
uroporphyrinogen III synthase/methyltransferase (NCBI)
Functional Annotations (10)
| Function | System |
|---|---|
| Uroporphyrinogen-III synthase | cog/ cog |
| diphthine synthase activity | go/ molecular_function |
| uroporphyrin-III C-methyltransferase activity | go/ molecular_function |
| uroporphyrinogen-III synthase activity | go/ molecular_function |
| porphyrin-containing compound biosynthetic process | go/ biological_process |
| peptidyl-diphthamide biosynthetic process from peptidyl-histidine | go/ biological_process |
| Porphyrin and chlorophyll metabolism | kegg/ kegg pathway |
| Metabolic pathways | kegg/ kegg pathway |
| Biosynthesis of secondary metabolites | kegg/ kegg pathway |
| cobA_cysG_Cterm | tigr/ tigrfam |
Module member 
Regulator 
Motif
Regulation information for GSU3286
(Mouseover regulator name to see its description)
| Regulator | Module | Operator |
|---|---|---|
| GSU0655 | 89 | tf |
| GSU1003 | 89 | tf |
| GSU1617 | 89 | tf |
| GSU2177 | 89 | tf |
| GSU2698 | 89 | tf |
| GSU3087 | 89 | tf |
| GSU3298 | 89 | tf |
| GSU3396 | 89 | tf |
| GSU0164 | 300 | tf |
| GSU1270 | 300 | tf |
| GSU2202 | 300 | tf |
| GSU2716 | 300 | tf |
| GSU2779 | 300 | tf |
| GSU3217 | 300 | tf |
Motif information (de novo identified motifs for modules)
There are 4 motifs predicted.
| Motif Id | e-value | Consensus | Motif Logo |
|---|---|---|---|
| 2338 | 2.30e-02 | C.aTGAAA.aaattACAaCAg |
 |
| 2339 | 1.10e-01 | aAAAcGCaAtCtTTgttTt |
|
| 2758 | 1.40e-07 | AaaTtgaCcc.tTCttccTCTaTC |
|
| 2759 | 1.00e-06 | CCGacCCTcCAGGtCGgCtAg |
|
Functional Enrichment for GSU3286
| Function | System |
|---|---|
| Uroporphyrinogen-III synthase | cog/ cog |
| diphthine synthase activity | go/ molecular_function |
| uroporphyrin-III C-methyltransferase activity | go/ molecular_function |
| uroporphyrinogen-III synthase activity | go/ molecular_function |
| porphyrin-containing compound biosynthetic process | go/ biological_process |
| peptidyl-diphthamide biosynthetic process from peptidyl-histidine | go/ biological_process |
| Porphyrin and chlorophyll metabolism | kegg/ kegg pathway |
| Metabolic pathways | kegg/ kegg pathway |
| Biosynthesis of secondary metabolites | kegg/ kegg pathway |
| cobA_cysG_Cterm | tigr/ tigrfam |
Module neighborhood information for GSU3286
| Gene | Common Name | Description | Module membership |
|---|---|---|---|
| GSU0015 | GSU0015 | PPIC-type PPIASE domain protein (NCBI) | 4, 89 |
| GSU0016 | ppiD | PPIC-type PPIASE domain protein (NCBI) | 4, 89 |
| GSU0018 | GSU0018 | transcriptional regulator, GntR family/aminotransferase class-I (VIMSS) | 4, 89 |
| GSU0019 | GSU0019 | pentapeptide repeat domain protein (VIMSS) | 4, 89 |
| GSU0044 | GSU0044 | salmonella virulence plasmid 65kDa B protein /YD repeat protein (NCBI) | 274, 300 |
| GSU0045 | GSU0045 | hypothetical protein (VIMSS) | 232, 300 |
| GSU0048 | GSU0048 | integrase (NCBI) | 287, 300 |
| GSU0223 | GSU0223 | protoheme IX farnesyl transferase, putative (VIMSS) | 257, 300 |
| GSU0369 | GSU0369 | FlhB domain protein, putative (NCBI) | 293, 300 |
| GSU0386 | radC | DNA repair protein RadC (NCBI) | 89, 283 |
| GSU0387 | GSU0387 | undecaprenol kinase, putative (VIMSS) | 89, 295 |
| GSU0388 | GSU0388 | conserved hypothetical protein (NCBI) | 89, 264 |
| GSU0389 | GSU0389 | phosphate transporter, putative (VIMSS) | 26, 89 |
| GSU0443 | GSU0443 | ribonuclease D, putative (VIMSS) | 89, 295 |
| GSU0529 | nfo | endonuclease IV (NCBI) | 89, 225 |
| GSU0595 | GSU0595 | hypothetical protein (NCBI) | 300, 337 |
| GSU0876 | GSU0876 | diacylglycerol kinase catalytic domain protein (NCBI) | 89, 322 |
| GSU1002 | GSU1002 | isochorismatase family protein (NCBI) | 89, 265 |
| GSU1003 | ntrC | nitrogen regulation protein NR(I) (NCBI) | 89, 265 |
| GSU1004 | GSU1004 | sensory box histidine kinase (VIMSS) | 89, 265 |
| GSU1034 | GSU1034 | None | 287, 300 |
| GSU1172 | mviN | virulence factor mviN protein (NCBI) | 295, 300 |
| GSU1270 | pyrR | pyrimidine operon regulatory protein PyrR (NCBI) | 281, 300 |
| GSU1329 | gltX | tRNA synthetases class I (E and Q), catalytic domain protein (NCBI) | 15, 300 |
| GSU1383 | dnaQ | exonuclease (NCBI) | 287, 300 |
| GSU1539 | xth | exodeoxyribonuclease III (NCBI) | 274, 300 |
| GSU1565 | GSU1565 | hypothetical protein (VIMSS) | 276, 300 |
| GSU1680 | GSU1680 | conserved hypothetical protein (VIMSS) | 59, 300 |
| GSU1703 | pfk | 6-phosphofructokinase (NCBI) | 204, 300 |
| GSU2080 | GSU2080 | rod shape-determining protein MreD, putative (VIMSS) | 137, 300 |
| GSU2166 | GSU2166 | hypothetical protein (VIMSS) | 1, 300 |
| GSU2204 | GSU2204 | cytochrome c family protein, putative (NCBI) | 300, 327 |
| GSU2205 | GSU2205 | hypothetical protein (VIMSS) | 300, 337 |
| GSU2336 | otsB | trehalose-phosphatase (NCBI) | 296, 300 |
| GSU2551 | GSU2551 | LysM domain protein (VIMSS) | 89, 231 |
| GSU2578 | cheW-9 | purine-binding chemotaxis protein CheW (NCBI) | 79, 300 |
| GSU2592 | GSU2592 | hypothetical protein (VIMSS) | 68, 300 |
| GSU2593 | GSU2593 | ISGsu6, transposase OrfA (VIMSS) | 234, 300 |
| GSU2594 | GSU2594 | ISGsu6, transposase OrfB (VIMSS) | 287, 300 |
| GSU2619 | tgt-2 | queuine tRNA-ribosyltransferase (NCBI) | 89, 231 |
| GSU2716 | sfsA | sugar fermentation stimulation protein (NCBI) | 226, 300 |
| GSU2890 | GSU2890 | cytochrome c biogenesis protein, CcmF/CcyK/CcsA family (VIMSS) | 130, 300 |
| GSU2957 | GSU2957 | thioredoxin family protein (VIMSS) | 146, 300 |
| GSU2989 | GSU2989 | L-threonine-O-3-phosphate decarboxylase, putative (VIMSS) | 89, 281 |
| GSU2991 | GSU2991 | sensor histidine kinase (VIMSS) | 89, 285 |
| GSU2992 | cobQ | cobyric acid synthase (NCBI) | 89, 153 |
| GSU2997 | cbiD | cobalamin biosynthesis protein CbiD (NCBI) | 89, 281 |
| GSU2998 | GSU2998 | nitroimidazole resistance protein, putative (VIMSS) | 89, 169 |
| GSU3002 | GSU3002 | cobalt ABC transporter, permease protein (VIMSS) | 89, 285 |
| GSU3004 | GSU3004 | cobalt transport protein CbiM (RefSeq) | 75, 89 |
| GSU3010 | cobU | bifunctional cobalamin biosynthesis protein CobU (NCBI) | 30, 89 |
| GSU3184 | GSU3184 | hypothetical protein (VIMSS) | 181, 300 |
| GSU3186 | GSU3186 | conserved hypothetical protein (VIMSS) | 213, 300 |
| GSU3264 | GSU3264 | membrane protein, putative (VIMSS) | 295, 300 |
| GSU3283 | GSU3283 | cytochrome c biogenesis protein, CcmF/CcyK/CcsA family (VIMSS) | 44, 89 |
| GSU3286 | hemD | uroporphyrinogen III synthase/methyltransferase (NCBI) | 89, 300 |
| GSU3287 | GSU3287 | hydrolase, TatD family (NCBI) | 30, 89 |
| GSU3288 | moeB | thiF family protein (NCBI) | 30, 89 |
Gene Page Help
Network Tab
If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.
Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.
Regulation Tab
Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.
If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.
You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".
For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.
Motifs Tab
Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.
Functions Tab
Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.
Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.
Module Members Tab
Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.
Help Tab
This help page. More general help can be accessed by clicking help menu in the main navigation bar.
CircVis
Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
- 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
- 2. Source gene
- 3. Target genes (other module members)
- 4. Interactions between source and target genes for a particular module
- 5. Module(s) that source gene and target genes belong to
- 6. Visualisation legend
Comments for GSU3286
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Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.
In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.