Organism : Halobacterium salinarum NRC-1 | Module List:
Module 45 Profile

GeneModule member RegulatorRegulator MotifMotif
Network Help

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges.

Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif
Regulators for Module 45

There are 4 regulatory influences for Module 45

Regulator Table (4)
Regulator Name Type
VNG2661G
VNG0424C		 combiner
VNG6389G tf
VNG6143H tf
VNG2661G
VNG1029C		 combiner

Regulator Help

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type.

tf: Transcription factor

ef: Environmental factor

combiner: Combinatorial influence of a tf or an ef through logic gate. Table is sortable by clicking on the arrows next to column headers.

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
1067 6.20e+01 AcaACGT
Loader icon
1068 6.80e+03 AATCAAaaTTGTtT
Loader icon
Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment

Regulon 45 is enriched for following functions.

KEGG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini Hochberg pvalue Genes with function Method
Glycolysis / Gluconeogenesis kegg pathway 9.00e-06 1.12e-04 4/32
Citrate cycle (TCA cycle) kegg pathway 0.00e+00 8.00e-06 5/32
Pyruvate metabolism kegg pathway 2.00e-06 3.40e-05 4/32
Oxidative phosphorylation kegg pathway 0.00e+00 0.00e+00 12/32
Methane metabolism kegg pathway 0.00e+00 0.00e+00 8/32
Porphyrin and chlorophyll metabolism kegg pathway 1.00e-06 1.30e-05 5/32
Metabolic pathways kegg pathway 0.00e+00 0.00e+00 24/32
Biosynthesis of secondary metabolites kegg pathway 2.76e-03 7.21e-03 6/32
Microbial metabolism in diverse environments kegg pathway 1.14e-03 4.18e-03 5/32
Carbohydrate Metabolism kegg subcategory 0.00e+00 0.00e+00 21/32
Energy Metabolism kegg subcategory 0.00e+00 0.00e+00 21/32
Metabolism of Cofactors and Vitamins kegg subcategory 4.85e-04 2.82e-03 5/32
Metabolism kegg subcategory 0.00e+00 0.00e+00 35/32
Metabolism kegg category 0.00e+00 0.00e+00 53/32
Global kegg category 0.00e+00 0.00e+00 35/32
Metabolism kegg category 0.00e+00 0.00e+00 24/32
Carbohydrate Metabolism kegg subcategory 7.50e-05 6.94e-04 6/32
Glycolysis / Gluconeogenesis kegg pathway 9.00e-06 1.13e-04 4/32
Citrate cycle (TCA cycle) kegg pathway 0.00e+00 7.00e-06 5/32
Pyruvate metabolism kegg pathway 2.00e-06 3.30e-05 4/32
Energy Metabolism kegg subcategory 0.00e+00 0.00e+00 13/32
Oxidative phosphorylation kegg pathway 0.00e+00 0.00e+00 12/32
Methane metabolism kegg pathway 0.00e+00 0.00e+00 8/32
Metabolism of Cofactors and Vitamins kegg subcategory 4.85e-04 2.88e-03 5/32
Porphyrin and chlorophyll metabolism kegg pathway 1.00e-06 1.30e-05 5/32
Global kegg category 0.00e+00 0.00e+00 24/32
Metabolism kegg subcategory 0.00e+00 0.00e+00 24/32
Metabolic pathways kegg pathway 0.00e+00 0.00e+00 24/32
Biosynthesis of secondary metabolites kegg pathway 2.76e-03 9.62e-03 6/32
Microbial metabolism in diverse environments kegg pathway 1.14e-03 5.43e-03 5/32

GO Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
electron transport biological_process 7.31e-04 1.68e-03 5/32
metabolic process biological_process 4.33e-03 6.97e-03 5/32
cobalamin biosynthetic process biological_process 5.70e-05 2.42e-04 4/32
ATP synthesis coupled proton transport biological_process 0.00e+00 0.00e+00 7/32
hydrogen-transporting two-sector ATPase activity molecular_function 0.00e+00 0.00e+00 8/32
NADH dehydrogenase (ubiquinone) activity molecular_function 0.00e+00 2.00e-06 4/32
hydrogen ion transporting ATP synthase activity, rotational mechanism molecular_function 0.00e+00 0.00e+00 8/32
proton-transporting ATPase activity, rotational mechanism molecular_function 0.00e+00 0.00e+00 8/32

TIGRFam Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Energy metabolism tigr mainrole 1.00e-06 1.10e-05 6/32
Energy metabolism tigr mainrole 1.00e-06 1.00e-06 6/32

COG Enrichment Table

Function Name Function Type Unadjusted pvalue Benjamini& Hochberg pvalue Genes with function Method
Energy production and conversion cog subcategory 0.00e+00 0.00e+00 15/32
Coenzyme transport and metabolism cog subcategory 1.83e-03 1.78e-02 5/32
Metabolism cog category 1.00e-06 2.80e-05 21/32
Metabolism cog category 2.00e-06 5.00e-06 20/32
Energy production and conversion cog subcategory 0.00e+00 0.00e+00 15/32
Coenzyme transport and metabolism cog subcategory 1.83e-03 3.29e-03 5/32
Functions Help

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Members for Module 45

There are 32 genes in Module 45

Gene Member Table (32)
Name Common name Type Gene ID Chromosome Start End Strand Description TF
VNG0043H CDS 1446988 chromosome 36321 36713 + hypothetical protein VNG0043H False
VNG0640G nolD CDS 1447447 chromosome 489042 489503 + NADH dehydrogenase/oxidoreductase-like protein False
VNG0641C CDS 1447448 chromosome 489561 489821 + NADH dehydrogenase subunit J False
VNG0642C CDS 1447449 chromosome 489893 490126 + hypothetical protein VNG0642C False
VNG0646G nuoL CDS 1447451 chromosome 490430 492508 + F420H2:quinone oxidoreductase subunit L False
VNG1048G udg1 CDS 1447756 chromosome 796773 798053 - UDP-glucose dehydrogenase False
VNG1542G sucD CDS 1448127 chromosome 1149527 1150396 + hypothetical protein VNG1542G False
VNG1550G cbiT CDS 1448134 chromosome 1156903 1157466 + cobalamin biosynthesis protein False
VNG1551G cbiL CDS 1448135 chromosome 1157463 1158167 + cobalt-precorrin-2 C(20)-methyltransferase False
VNG1553G cbiF CDS 1448136 chromosome 1158160 1159026 + cobalamin biosynthesis protein False
VNG1554G cbiG CDS 1448137 chromosome 1159023 1160000 + cobalamin biosynthesis protein CbiG False
VNG1557G cbiH CDS 1448139 chromosome 1160827 1161834 + cobalamin biosynthesis protein False
VNG1558H CDS 1448140 chromosome 1161834 1162082 + hypothetical protein VNG1558H False
VNG1559H CDS 1448141 chromosome 1162079 1162777 + hypothetical protein VNG1559H False
VNG1562H CDS 1448142 chromosome 1163994 1164365 + hypothetical protein VNG1562H False
VNG2099C CDS 1448548 chromosome 1542347 1542730 - hypothetical protein VNG2099C False
VNG2138G atpB CDS 1448579 chromosome 1572295 1573710 - V-type ATP synthase subunit B False
VNG2139G atpA CDS 1448580 chromosome 1573716 1575473 - V-type ATP synthase subunit A False
VNG2140G atpF CDS 1448581 chromosome 1575479 1575841 - V-type ATP synthase subunit F False
VNG2141G atpC CDS 1448582 chromosome 1575838 1576896 - V-type ATP synthase subunit C False
VNG2142G atpE CDS 1448583 chromosome 1576893 1577480 - V-type ATP synthase subunit E False
VNG2143G atpK CDS 1448584 chromosome 1577503 1577718 - H+-transporting ATP synthase subunit K False
VNG2144G atpI CDS 1448585 chromosome 1577784 1579952 - H+-transporting ATP synthase subunit I False
VNG2146H CDS 1448586 chromosome 1579939 1580271 - hypothetical protein VNG2146H False
VNG2150G etfB CDS 1448590 chromosome 1584077 1584877 + electron transfer flavoprotein subunit beta False
VNG2151G etfA CDS 1448591 chromosome 1584874 1585842 + electron transfer flavoprotein subunit alpha False
VNG2217G pdhA2 CDS 1448646 chromosome 1646140 1647399 + pyruvate dehydrogenase alpha subunit False
VNG2218G pdhB CDS 1448647 chromosome 1647485 1648378 + hypothetical protein VNG2218G False
VNG2219G dsa CDS 1448648 chromosome 1648380 1649816 + branched-chain alpha-keto acid dehydrogenase subunit E2 False
VNG2220G lpdA CDS 1448649 chromosome 1649819 1651243 + LpdA False
VNG2658G rps12P CDS 1448995 chromosome 1993547 1993975 - 30S ribosomal protein S12P False
VNG2662G rpoC CDS 1448997 chromosome 1994558 1995751 - DNA-directed RNA polymerase subunit A'' False

Genes Help

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Help

What is a module?

Regulatory units (modules) in the Network Portal are based on the network inference algorithm used. For the current version, modules are based on cMonkey modules and Inferelator regulatory influences on these modules. More specifically, module refers to set of genes that are conditionally co-regulated under subset of the conditions. Identification of modules integrates co-expression, de-novo motif identification, and other functional associations such as operon information and protein-protein interactions.

Module Overview

The landing module page shows quick summary info including co-expression profiles, de-novo identified motifs, and transcription factors and/or environmental factors as regulatory influences. It also includes module residual, motif e-values, conditions and links to other resources such as NCBI and Microbesonline. . If a transcription factor is included in the manually curated RegPrecise database, further information from RegPrecise is shown, allowing users to perform comparative analysis.

Expression Profiles

Expression profiles is a plot of the expression ratios (log10) of the module's genes, over all subset of the conditions included in the module. The X-axis represent conditions and the Y-axis represents log10 expression ratios. Each gene is plotted as line plot with different colors. Colored legend for the lines are presented under the plot. This plot is dynamic. Clicking on the gene names in the legend will show/hide the plot for that particular gene. A tooltip will show expression ratio information if you mouseover the lines in the plot.

Motif Locations

Location of the Identified motifs for the module in the upstream regions of the member genes are shown under the expression profiles plot. This plot shows the diagram of the upstream positions of the motifs, colored red and green for motifs #1, and 2, respectively. Intensity of the color is proportional to the significance of the occurence of that motif at a given location. Motifs on the forward and reverse strand are represented over and under the line respectively.

Network

A network view of the module is created using cytoscapeWeb and enables dynamic, interactive exploration of the module properties. In this view, module member genes, motifs, and regulatory influences are represented as peripheral nodes connected to core module node via edges. Module members are green circles, regulators are red triangles and motifs are blue diamonds. Selection of a node gives access to detailed information in a pop-up window, which allows dragging and pinning to compare multiple selections. Selecting module members will show information about the selected gene such as name, species and fucntions. Motif selection will show motif logo image and e-values. Bicluster selction will show expression profile and summary statistics for the module.

GeneModule member RegulatorRegulator MotifMotif

Regulators

For each module, single or AND logic connected regulatory influences are listed under the regulators tab. These regulatory influences are identified by Inferelator. Table shows name of the regulator and its type. tf: Transcription factor, ef: Environmental factor and combiner:Combinatorial influence of a tf or an ef through logic gate. Tabel is sortable by clicking on the arrows next to column headers.

Motifs

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Functions

Biological networks contain sets of regulatory units called functional modules that together play a role in regulation of specific functional processes. Connections between different modules in the network can help identify regulatory relationships such as hierarchy and epistasis. In addition, associating functions with modules enables putative assignment of functions to hypothetical genes. It is therefore essential to identify functional enrichment of modules within the regulatory network.

Functional annotations from single sources are often either not available or not complete. Therefore, we integrated KEGG pathway, Gene Ontology, TIGRFam and COG information as references for functional enrichment analysis.

We use hypergeometric p-values to identify significant overlaps between co-regulated module members and genes assigned to a particular functional annotation category. P-values are corrected for multiple comparisons by using Benjamini-Hochberg correction and filtered for p-values ≤ 0.05.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Genes

Gene member table shows all the genes included in the module. Listed attributes are;

  1. Name: Gene name or Locus tag
  2. Common Name: Gene short name
  3. Type: Type of the feature, usually CDS.
  4. Gene ID: Link to NCBI Gene ID
  5. Chromosome: Chromosome name from annotation file
  6. Start/End:Feature start and end coordinates
  7. Strand: strand of the gene
  8. Description: Description of the gene from annotation file
  9. TF: If the gene is a Transcription Factor or not.

If you are browsing the Network Portal by using Gaggle/Firegoose, firegoose plugin will capture the NameList of the gene members. Captured names can be saved into your Workspace by clicking on "Capture" in the firegoose toolbar or can be directly sent other desktop and web resources by using "Broadcast" option.

Social

You can start a conversation about this module or join the existing discussion by adding your comments. In order to be able to add your comments you need to sign in by using any of the following services;Disqus, Google, Facebook or Twitter. For full compatibility with other network portal features, we recommend using your Google ID.

Definitions

Residual: is a measure of bicluster quality. Mean bicluster residual is smaller when the expression profile of the genes in the module is "tighter". So smaller residuals are usually indicative of better bicluster quality.

Expression Profile: is a preview of the expression profiles of all the genes under subset of conditions included in the module. Tighter expression profiles are usually indicative of better bicluster quality.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Genes: Number of genes included in the module.

Functions: We identify functional enrichment of each module by camparing to different functional categories such as KEGG, COG, GO etc. by using hypergeometric function. If the module is significantly enriched for any of the functions, this column will list few of the these functions as an overview. Full list of functions is available upon visiting the module page under the Functions tab.