Organism : Pseudomonas aeruginosa | Module List :
PA2232 pslB

probable phosphomannose isomerase/GDP-mannose pyrophosphorylase (NCBI)

CircVis
Functional Annotations (8)
Function System
Mannose-1-phosphate guanylyltransferase cog/ cog
mannose-1-phosphate guanylyltransferase (GDP) activity go/ molecular_function
lipopolysaccharide biosynthetic process go/ biological_process
Fructose and mannose metabolism kegg/ kegg pathway
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
GMP_PMI tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2232
(Mouseover regulator name to see its description)

PA2232 is regulated by 57 influences and regulates 0 modules.
Regulators for PA2232 pslB (57)
Regulator Module Operator
PA0289 300 tf
PA0376 300 tf
PA0393 300 tf
PA0416 300 tf
PA0475 300 tf
PA0477 300 tf
PA0610 300 tf
PA0791 300 tf
PA1153 300 tf
PA1229 300 tf
PA1374 300 tf
PA1413 300 tf
PA1430 300 tf
PA1526 300 tf
PA1619 300 tf
PA2016 300 tf
PA2020 300 tf
PA2227 300 tf
PA2383 300 tf
PA2588 300 tf
PA2736 300 tf
PA2917 300 tf
PA2930 300 tf
PA3067 300 tf
PA3126 300 tf
PA3135 300 tf
PA3433 300 tf
PA3565 300 tf
PA3571 300 tf
PA3879 300 tf
PA3921 300 tf
PA4132 300 tf
PA4227 300 tf
PA4530 300 tf
PA4547 300 tf
PA4596 300 tf
PA4600 300 tf
PA4984 300 tf
PA0034 522 tf
PA0179 522 tf
PA0225 522 tf
PA0289 522 tf
PA0491 522 tf
PA0707 522 tf
PA0893 522 tf
PA1097 522 tf
PA1229 522 tf
PA1430 522 tf
PA1526 522 tf
PA2586 522 tf
PA2588 522 tf
PA2879 522 tf
PA3002 522 tf
PA3599 522 tf
PA3689 522 tf
PA4547 522 tf
PA5344 522 tf

Warning: PA2232 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
3858 6.10e+01 GtAAcagcctgt.TTacaCttcCC
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3859 4.50e+03 TGGATAAaA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2232

PA2232 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
Mannose-1-phosphate guanylyltransferase cog/ cog
mannose-1-phosphate guanylyltransferase (GDP) activity go/ molecular_function
lipopolysaccharide biosynthetic process go/ biological_process
Fructose and mannose metabolism kegg/ kegg pathway
Amino sugar and nucleotide sugar metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
GMP_PMI tigr/ tigrfam
Module neighborhood information for PA2232

PA2232 has total of 26 gene neighbors in modules 300, 522
Gene neighbors (26)
Gene Common Name Description Module membership
PA0598 PA0598 hypothetical protein (NCBI) 452, 522
PA1460 motC flagellar motor protein (NCBI) 129, 522
PA1461 motD flagellar motor protein (NCBI) 129, 522
PA1473 PA1473 hypothetical protein (NCBI) 3, 522
PA1526 PA1526 probable transcriptional regulator (NCBI) 62, 522
PA1527 PA1527 hypothetical protein (NCBI) 202, 522
PA2231 pslA probable glycosyl transferase (NCBI) 300, 522
PA2232 pslB probable phosphomannose isomerase/GDP-mannose pyrophosphorylase (NCBI) 300, 522
PA2233 pslC probable glycosyl transferase (NCBI) 300, 522
PA2234 pslD probable exopolysaccharide transporter (NCBI) 300, 522
PA2235 pslE hypothetical protein (NCBI) 300, 522
PA2236 pslF hypothetical protein (NCBI) 300, 522
PA2237 pslG probable glycosyl hydrolase (NCBI) 300, 522
PA2238 pslH hypothetical protein (NCBI) 300, 522
PA2239 pslI probable transferase (NCBI) 300, 522
PA2240 pslJ hypothetical protein (NCBI) 300, 522
PA2241 pslK hypothetical protein (NCBI) 289, 522
PA2242 pslL hypothetical protein (NCBI) 300, 522
PA3020 PA3020 probable soluble lytic transglycosylase (NCBI) 62, 522
PA4550 fimU type 4 fimbrial biogenesis protein FimU (NCBI) 168, 300
PA4551 pilV type 4 fimbrial biogenesis protein PilV (NCBI) 168, 300
PA4552 pilW type 4 fimbrial biogenesis protein PilW (NCBI) 168, 300
PA4553 pilX type 4 fimbrial biogenesis protein PilX (NCBI) 168, 300
PA4554 pilY1 type 4 fimbrial biogenesis protein PilY1 (NCBI) 168, 300
PA4555 pilY2 type 4 fimbrial biogenesis protein PilY2 (NCBI) 168, 300
PA4556 pilE type 4 fimbrial biogenesis protein PilE (NCBI) 168, 300
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2232
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend