Organism : Pseudomonas aeruginosa | Module List :
PA0475

probable transcriptional regulator (NCBI)

CircVis
Functional Annotations (4)
Function System
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
specific transcriptional repressor activity go/ molecular_function
negative regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0475
(Mouseover regulator name to see its description)

PA0475 is regulated by 24 influences and regulates 9 modules.
Regulators for PA0475 (24)
Regulator Module Operator
PA0289 443 tf
PA0475 443 tf
PA0533 443 tf
PA0815 443 tf
PA1315 443 tf
PA1760 443 tf
PA2047 443 tf
PA2123 443 tf
PA2259 443 tf
PA3363 443 tf
PA3864 443 tf
PA4145 443 tf
PA4703 443 tf
PA5059 443 tf
PA5437 443 tf
PA0032 171 tf
PA0701 171 tf
PA1399 171 tf
PA2093 171 tf
PA3381 171 tf
PA3596 171 tf
PA3776 171 tf
PA3830 171 tf
PA4238 171 tf
Regulated by PA0475 (9)
Module Residual Genes
67 0.46 17
160 0.42 13
300 0.44 18
347 0.34 11
365 0.49 22
422 0.52 21
443 0.54 36
483 0.49 32
523 0.53 33
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3170 3.00e+03 cAgCGcCAgCG
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3171 7.50e+03 ATATTTTTaAT
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3706 1.30e+02 atTCcTCgTgcAtaGgcTgGa
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3707 4.00e+04 gcGgCGgCgcggcGCttGccGaG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0475

PA0475 is enriched for 4 functions in 2 categories.
Enrichment Table (4)
Function System
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
specific transcriptional repressor activity go/ molecular_function
negative regulation of transcription, DNA-dependent go/ biological_process
Module neighborhood information for PA0475

PA0475 has total of 61 gene neighbors in modules 171, 443
Gene neighbors (61)
Gene Common Name Description Module membership
PA0144 PA0144 hypothetical protein (NCBI) 291, 443
PA0145 PA0145 hypothetical protein (NCBI) 171, 333
PA0289 gpuR transcriptional activator GpuR (NCBI) 443, 469
PA0327 PA0327 hypothetical protein (NCBI) 171, 189
PA0442 PA0442 hypothetical protein (NCBI) 171, 340
PA0458 PA0458 probable major facilitator superfamily (MFS) transporter (NCBI) 3, 443
PA0475 PA0475 probable transcriptional regulator (NCBI) 171, 443
PA0657 PA0657 probable ATPase (NCBI) 443, 523
PA0704 PA0704 amidase (NCBI) 388, 443
PA0808 PA0808 hypothetical protein (NCBI) 163, 443
PA0813 PA0813 hypothetical protein (NCBI) 243, 443
PA0815 PA0815 probable transcriptional regulator (NCBI) 243, 443
PA0957 PA0957 hypothetical protein (NCBI) 33, 171
PA1063 PA1063 hypothetical protein (NCBI) 203, 443
PA1133 PA1133 hypothetical protein (NCBI) 171, 241
PA1521 PA1521 probable guanine deaminase (NCBI) 163, 443
PA1680 PA1680 hypothetical protein (NCBI) 206, 443
PA1762 PA1762 hypothetical protein (NCBI) 443, 489
PA1763 PA1763 hypothetical protein (NCBI) 443, 483
PA1765 PA1765 hypothetical protein (NCBI) 153, 443
PA1909 PA1909 hypothetical protein (NCBI) 171, 486
PA2121 PA2121 probable transcriptional regulator (NCBI) 252, 443
PA2123 PA2123 probable transcriptional regulator (NCBI) 443, 483
PA2546 PA2546 probable ring-cleaving dioxygenase (NCBI) 171, 526
PA2772 PA2772 hypothetical protein (NCBI) 156, 171
PA3051 PA3051 hypothetical protein (NCBI) 26, 171
PA3122 PA3122 probable transcriptional regulator (NCBI) 171, 477
PA3316 PA3316 probable permease of ABC transporter (NCBI) 171, 387
PA3343 PA3343 hypothetical protein (NCBI) 203, 443
PA3358 PA3358 hypothetical protein (NCBI) 171, 241
PA3365 PA3365 probable chaperone (NCBI) 34, 171
PA3404 PA3404 probable outer membrane protein precursor (NCBI) 132, 171
PA3405 hasE metalloprotease secretion protein (NCBI) 132, 171
PA3425 PA3425 hypothetical protein (NCBI) 443, 483
PA3426 PA3426 enoyl-CoA hydratase (NCBI) 28, 443
PA3462 PA3462 probable sensor/response regulator hybrid (NCBI) 171, 345
PA3682 PA3682 hypothetical protein (NCBI) 171, 498
PA3715 PA3715 hypothetical protein (NCBI) 101, 443
PA3847 PA3847 hypothetical protein (NCBI) 397, 443
PA3856 PA3856 hypothetical protein (NCBI) 378, 443
PA3943 PA3943 hypothetical protein (NCBI) 443, 546
PA3944 PA3944 hypothetical protein (NCBI) 378, 443
PA3953 PA3953 hypothetical protein (NCBI) 171, 489
PA4074 PA4074 probable transcriptional regulator (NCBI) 171, 445
PA4091 hpaA 4-hydroxyphenylacetate 3-monooxygenase large chain (NCBI) 171, 483
PA4147 acoR transcriptional regulator AcoR (NCBI) 236, 443
PA4199 PA4199 probable acyl-CoA dehydrogenase (NCBI) 3, 443
PA4201 ddlA D-alanine-D-alanine ligase A (NCBI) 443, 469
PA4282 PA4282 probable exonuclease (NCBI) 443, 523
PA4330 PA4330 probable enoyl-CoA hydratase/isomerase (NCBI) 192, 443
PA4331 PA4331 hypothetical protein (NCBI) 62, 443
PA4332 PA4332 hypothetical protein (NCBI) 62, 443
PA4335 PA4335 hypothetical protein (NCBI) 443, 462
PA4346 PA4346 hypothetical protein (NCBI) 171, 483
PA4397 panE 2-dehydropantoate 2-reductase (NCBI) 10, 443
PA4875 PA4875 hypothetical protein (NCBI) 397, 443
PA5023 PA5023 hypothetical protein (NCBI) 31, 171
PA5026 PA5026 hypothetical protein (NCBI) 53, 171
PA5103 PA5103 hypothetical protein (NCBI) 171, 196
PA5272 cyaA adenylate cyclase (NCBI) 127, 443
PA5386 PA5386 3-hydroxybutyryl-CoA dehydrogenase (NCBI) 171, 534
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0475
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend