Organism : Pseudomonas aeruginosa | Module List :
PA2270

probable transcriptional regulator (NCBI)

CircVis
Functional Annotations (5)
Function System
Transcriptional regulator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
specific transcriptional repressor activity go/ molecular_function
negative regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2270
(Mouseover regulator name to see its description)

PA2270 is regulated by 34 influences and regulates 5 modules.
Regulators for PA2270 (34)
Regulator Module Operator
PA0037 351 tf
PA0152 351 tf
PA0253 351 tf
PA0831 351 tf
PA1241 351 tf
PA1290 351 tf
PA1630 351 tf
PA2115 351 tf
PA2270 351 tf
PA2447 351 tf
PA2510 351 tf
PA2921 351 tf
PA3322 351 tf
PA3604 351 tf
PA4451 351 tf
PA4890 351 tf
PA5356 351 tf
PA0514 257 tf
PA2259 257 tf
PA2267 257 tf
PA2270 257 tf
PA2299 257 tf
PA2312 257 tf
PA2332 257 tf
PA2337 257 tf
PA2359 257 tf
PA2383 257 tf
PA2387 257 tf
PA2417 257 tf
PA2423 257 tf
PA2484 257 tf
PA2492 257 tf
PA2692 257 tf
PA4203 257 tf
Regulated by PA2270 (5)
Module Residual Genes
27 0.50 22
257 0.52 23
339 0.52 23
351 0.53 26
494 0.51 22
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3342 1.30e-11 AAaAataCcc.tCaataTT.aaAa
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3343 1.70e-03 AAcataaTg.tTcga.ACtgc
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3526 2.00e-03 CgcCG.TcCgcCAt.CcttgtaG
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3527 6.60e+00 tTcG.CaAcGC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2270

PA2270 is enriched for 5 functions in 3 categories.
Module neighborhood information for PA2270

PA2270 has total of 48 gene neighbors in modules 257, 351
Gene neighbors (48)
Gene Common Name Description Module membership
PA0152 pcaQ transcriptional regulator PcaQ (NCBI) 351, 536
PA0253 PA0253 probable transcriptional regulator (NCBI) 181, 351
PA0350 folA dihydrofolate reductase (NCBI) 58, 351
PA0463 creB two-component response regulator CreB (NCBI) 58, 351
PA0464 creC two-component sensor CreC (NCBI) 351, 507
PA1332 PA1332 hypothetical protein (NCBI) 108, 351
PA1375 pdxB erythronate-4-phosphate dehydrogenase (NCBI) 351, 490
PA1965 PA1965 hypothetical protein (NCBI) 139, 351
PA2270 PA2270 probable transcriptional regulator (NCBI) 257, 351
PA2272 pbpC penicillin-binding protein 3A (NCBI) 257, 494
PA2301 PA2301 hypothetical protein (NCBI) 34, 257
PA2306 PA2306 hypothetical protein (NCBI) 257, 338
PA2327 PA2327 probable permease of ABC transporter (NCBI) 176, 257
PA2328 PA2328 hypothetical protein (NCBI) 176, 257
PA2329 PA2329 probable ATP-binding component of ABC transporter (NCBI) 176, 257
PA2330 PA2330 hypothetical protein (NCBI) 176, 257
PA2331 PA2331 hypothetical protein (NCBI) 176, 257
PA2332 PA2332 probable transcriptional regulator (NCBI) 257, 494
PA2378 PA2378 probable aldehyde dehydrogenase (NCBI) 257, 338
PA2379 PA2379 probable oxidoreductase (NCBI) 257, 338
PA2380 PA2380 hypothetical protein (NCBI) 257, 494
PA2383 PA2383 probable transcriptional regulator (NCBI) 257, 494
PA2387 PA2387 probable sigma-70 factor, ECF subfamily (NCBI) 257, 494
PA2388 PA2388 probable transmembrane sensor (NCBI) 257, 494
PA2417 PA2417 probable transcriptional regulator (NCBI) 257, 494
PA2423 PA2423 hypothetical protein (NCBI) 257, 494
PA2436 PA2436 hypothetical protein (NCBI) 34, 257
PA2450 PA2450 hypothetical protein (NCBI) 139, 351
PA2464 PA2464 hypothetical protein (NCBI) 257, 494
PA2483 PA2483 hypothetical protein (NCBI) 257, 505
PA2491 PA2491 probable oxidoreductase (NCBI) 257, 494
PA2492 mexT transcriptional regulator MexT (NCBI) 257, 494
PA2502 PA2502 hypothetical protein (NCBI) 135, 351
PA2503 PA2503 hypothetical protein (NCBI) 351, 490
PA2510 catR transcriptional regulator CatR (NCBI) 307, 351
PA2855 PA2855 hypothetical protein (NCBI) 230, 351
PA2921 PA2921 probable transcriptional regulator (NCBI) 61, 351
PA3388 PA3388 hypothetical protein (NCBI) 299, 351
PA3473 PA3473 hypothetical protein (NCBI) 351, 377
PA3474 PA3474 hypothetical protein (NCBI) 351, 377
PA3949 PA3949 hypothetical protein (NCBI) 351, 544
PA4013 PA4013 hypothetical protein (NCBI) 351, 422
PA4014 PA4014 hypothetical protein (NCBI) 58, 351
PA4634 PA4634 hypothetical protein (NCBI) 299, 351
PA4789 PA4789 hypothetical protein (NCBI) 229, 351
PA4790 PA4790 hypothetical protein (NCBI) 229, 351
PA5150 PA5150 probable short-chain dehydrogenase (NCBI) 190, 351
PA5151 PA5151 hypothetical protein (NCBI) 190, 351
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2270
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend