Organism : Pseudomonas aeruginosa | Module List :
PA4089

probable short-chain dehydrogenase (NCBI)

CircVis
Functional Annotations (7)
Function System
Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) cog/ cog
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity go/ molecular_function
metabolic process go/ biological_process
oxidoreductase activity go/ molecular_function
Fatty acid biosynthesis kegg/ kegg pathway
Biosynthesis of unsaturated fatty acids kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4089
(Mouseover regulator name to see its description)

PA4089 is regulated by 51 influences and regulates 0 modules.
Regulators for PA4089 (51)
Regulator Module Operator
PA0191 285 tf
PA0512 285 tf
PA0701 285 tf
PA0748 285 tf
PA1347 285 tf
PA1949 285 tf
PA1980 285 tf
PA2258 285 tf
PA2276 285 tf
PA2354 285 tf
PA2547 285 tf
PA2838 285 tf
PA3045 285 tf
PA3381 285 tf
PA3420 285 tf
PA3596 285 tf
PA3630 285 tf
PA3711 285 tf
PA4341 285 tf
PA4989 285 tf
PA5562 285 tf
PA0056 36 tf
PA0191 36 tf
PA0306 36 tf
PA0393 36 tf
PA0512 36 tf
PA0701 36 tf
PA0748 36 tf
PA0784 36 tf
PA0893 36 tf
PA1285 36 tf
PA1347 36 tf
PA1351 36 tf
PA1949 36 tf
PA1980 36 tf
PA2276 36 tf
PA2547 36 tf
PA3045 36 tf
PA3249 36 tf
PA3381 36 tf
PA3420 36 tf
PA3596 36 tf
PA3711 36 tf
PA3771 36 tf
PA3932 36 tf
PA4074 36 tf
PA4341 36 tf
PA5032 36 tf
PA5293 36 tf
PA5382 36 tf
PA5562 36 tf

Warning: PA4089 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2904 8.20e-02 aAcaAcaAC.aat..AAgaat
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2905 6.50e+03 AcaTCatcaA.cAgt
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3398 1.50e-03 aAcaAcaaCAA
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3399 1.10e+04 tATttCcgTtG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4089

PA4089 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) cog/ cog
3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity go/ molecular_function
metabolic process go/ biological_process
oxidoreductase activity go/ molecular_function
Fatty acid biosynthesis kegg/ kegg pathway
Biosynthesis of unsaturated fatty acids kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Module neighborhood information for PA4089

PA4089 has total of 34 gene neighbors in modules 36, 285
Gene neighbors (34)
Gene Common Name Description Module membership
PA0189 PA0189 probable porin (NCBI) 36, 201
PA0439 PA0439 dihydropyrimidine dehydrogenase (NCBI) 285, 481
PA0440 PA0440 probable oxidoreductase (NCBI) 285, 481
PA0480 PA0480 probable hydrolase (NCBI) 172, 285
PA1025 PA1025 probable porin (NCBI) 36, 425
PA1267 PA1267 hypothetical protein (NCBI) 16, 36
PA1346 PA1346 hypothetical protein (NCBI) 36, 158
PA2124 PA2124 probable dehydrogenase (NCBI) 12, 36
PA2129 cupA2 chaperone CupA2 (NCBI) 36, 439
PA2133 PA2133 hypothetical protein (NCBI) 36, 285
PA2214 PA2214 probable major facilitator superfamily (MFS) transporter (NCBI) 148, 285
PA2215 PA2215 hypothetical protein (NCBI) 285, 428
PA2217 PA2217 probable aldehyde dehydrogenase (NCBI) 36, 340
PA2348 PA2348 hypothetical protein (NCBI) 285, 304
PA2472 PA2472 probable major facilitator superfamily (MFS) transporter (NCBI) 285, 304
PA2474 PA2474 hypothetical protein (NCBI) 285, 304
PA2516 xylZ toluate 1,2-dioxygenase electron transfer component (NCBI) 172, 285
PA2517 xylY toluate 1,2-dioxygenase beta subunit (NCBI) 172, 285
PA2518 xylX toluate 1,2-dioxygenase alpha subunit (NCBI) 172, 285
PA2924 hisQ histidine transport system permease HisQ (NCBI) 36, 285
PA2926 hisP histidine transport protein HisP (NCBI) 92, 285
PA3395 nosY NosY protein (NCBI) 36, 497
PA3589 PA3589 acetyl-CoA acetyltransferase (NCBI) 36, 463
PA3591 PA3591 probable enoyl-CoA hydratase/isomerase (NCBI) 36, 463
PA4071 PA4071 hypothetical protein (NCBI) 36, 285
PA4072 PA4072 probable amino acid permease (NCBI) 36, 285
PA4083 cupB4 chaperone CupB4 (NCBI) 36, 439
PA4084 cupB3 usher CupB3 (NCBI) 12, 36
PA4085 cupB2 chaperone CupB2 (NCBI) 158, 285
PA4087 PA4087 hypothetical protein (NCBI) 235, 285
PA4088 PA4088 probable aminotransferase (NCBI) 36, 285
PA4089 PA4089 probable short-chain dehydrogenase (NCBI) 36, 285
PA4100 PA4100 probable dehydrogenase (NCBI) 125, 285
PA4137 PA4137 probable porin (NCBI) 36, 177
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4089
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend