Organism : Pseudomonas aeruginosa | Module List :
PA4917

hypothetical protein (NCBI)

CircVis
Functional Annotations (4)
Function System
Nicotinic acid mononucleotide adenylyltransferase cog/ cog
biosynthetic process go/ biological_process
nucleotidyltransferase activity go/ molecular_function
cyt_tran_rel tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4917
(Mouseover regulator name to see its description)

PA4917 is regulated by 57 influences and regulates 0 modules.
Regulators for PA4917 (57)
Regulator Module Operator
PA0424 278 tf
PA0576 278 tf
PA0839 278 tf
PA1196 278 tf
PA1455 278 tf
PA1526 278 tf
PA2577 278 tf
PA2885 278 tf
PA3002 278 tf
PA3007 278 tf
PA3458 278 tf
PA3948 278 tf
PA3973 278 tf
PA4052 278 tf
PA4057 278 tf
PA4269 278 tf
PA4279 278 tf
PA4530 278 tf
PA4745 278 tf
PA5308 278 tf
PA0120 216 tf
PA0762 216 tf
PA0815 216 tf
PA0876 216 tf
PA0893 216 tf
PA1003 216 tf
PA1097 216 tf
PA1128 216 tf
PA1201 216 tf
PA1455 216 tf
PA1484 216 tf
PA1504 216 tf
PA1526 216 tf
PA1850 216 tf
PA1978 216 tf
PA2020 216 tf
PA2032 216 tf
PA2206 216 tf
PA2492 216 tf
PA2511 216 tf
PA2556 216 tf
PA2601 216 tf
PA2718 216 tf
PA2885 216 tf
PA2957 216 tf
PA3124 216 tf
PA3225 216 tf
PA3266 216 tf
PA3398 216 tf
PA3458 216 tf
PA3804 216 tf
PA4147 216 tf
PA4182 216 tf
PA4493 216 tf
PA4530 216 tf
PA4787 216 tf
PA5562 216 tf

Warning: PA4917 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3260 4.30e-03 TTCatCACgtgAaAtAAATCgtCA
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3261 4.30e-03 AAAAtaCCTTcCActgCGaAatTT
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3384 1.80e-05 A.aAaAtAccatggactgCGaAAt
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3385 6.90e-01 atgtattgcACgTgatGaAttAaG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4917

PA4917 is enriched for 4 functions in 3 categories.
Enrichment Table (4)
Function System
Nicotinic acid mononucleotide adenylyltransferase cog/ cog
biosynthetic process go/ biological_process
nucleotidyltransferase activity go/ molecular_function
cyt_tran_rel tigr/ tigrfam
Module neighborhood information for PA4917

PA4917 has total of 33 gene neighbors in modules 216, 278
Gene neighbors (33)
Gene Common Name Description Module membership
PA0404 PA0404 Holliday junction resolvase-like protein (NCBI) 86, 278
PA0596 PA0596 hypothetical protein (NCBI) 278, 452
PA0869 pbpG D-alanyl-D-alanine-endopeptidase (NCBI) 82, 278
PA2744 thrS threonyl-tRNA synthetase (NCBI) 278, 395
PA3141 wbpM nucleotide sugar epimerase/dehydratase WbpM (NCBI) 278, 488
PA3435 PA3435 flavodoxin (NCBI) 278, 541
PA3566 PA3566 hypothetical protein (NCBI) 225, 278
PA3567 PA3567 probable oxidoreductase (NCBI) 225, 278
PA3657 map methionine aminopeptidase (NCBI) 228, 278
PA3978 PA3978 hypothetical protein (NCBI) 278, 452
PA4057 PA4057 hypothetical protein (NCBI) 228, 278
PA4407 ftsZ cell division protein FtsZ (NCBI) 15, 216
PA4408 ftsA cell division protein FtsA (NCBI) 67, 216
PA4409 ftsQ cell division protein FtsQ (NCBI) 67, 216
PA4410 ddlB D-alanylalanine synthetase (NCBI) 67, 216
PA4411 murC UDP-N-acetylmuramate--L-alanine ligase (NCBI) 67, 216
PA4412 murG N-acetylglucosaminyl transferase (NCBI) 67, 216
PA4413 ftsW cell division protein FtsW (NCBI) 67, 216
PA4414 murD UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) 67, 216
PA4415 mraY phospho-N-acetylmuramoyl-pentapeptide-transferase (NCBI) 67, 216
PA4416 murF UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase (NCBI) 67, 216
PA4417 murE UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (NCBI) 67, 216
PA4418 ftsI penicillin-binding protein 3 (NCBI) 67, 216
PA4419 ftsL cell division protein FtsL (NCBI) 67, 216
PA4420 mraW S-adenosyl-methyltransferase (NCBI) 67, 216
PA4421 PA4421 hypothetical protein (NCBI) 67, 216
PA4916 PA4916 hypothetical protein (NCBI) 216, 278
PA4917 PA4917 hypothetical protein (NCBI) 216, 278
PA4918 PA4918 hypothetical protein (NCBI) 216, 278
PA4919 pncB1 nicotinate phosphoribosyltransferase (NCBI) 216, 278
PA4920 nadE NAD(+) synthetase (NCBI) 216, 278
PA4943 PA4943 probable GTP-binding protein (NCBI) 278, 409
PA5492 PA5492 GTP-binding protein (NCBI) 129, 278
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4917
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend