Organism : Pseudomonas aeruginosa | Module List :
PA5423

hypothetical protein (NCBI)

CircVis
Functional Annotations (1)
Function System
Uncharacterized protein conserved in bacteria cog/ cog
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA5423
(Mouseover regulator name to see its description)

PA5423 is regulated by 39 influences and regulates 0 modules.
Regulators for PA5423 (39)
Regulator Module Operator
PA0034 221 tf
PA0610 221 tf
PA0652 221 tf
PA0762 221 tf
PA1097 221 tf
PA1099 221 tf
PA1226 221 tf
PA1397 221 tf
PA2258 221 tf
PA2387 221 tf
PA2469 221 tf
PA2577 221 tf
PA2896 221 tf
PA3067 221 tf
PA3269 221 tf
PA3604 221 tf
PA3699 221 tf
PA4296 221 tf
PA5085 221 tf
PA5261 221 tf
PA5324 221 tf
PA5483 221 tf
PA0179 457 tf
PA0601 457 tf
PA0763 457 tf
PA0815 457 tf
PA0942 457 tf
PA1125 457 tf
PA1603 457 tf
PA1607 457 tf
PA2718 457 tf
PA2897 457 tf
PA2899 457 tf
PA3034 457 tf
PA3477 457 tf
PA3689 457 tf
PA5261 457 tf
PA5288 457 tf
PA5428 457 tf

Warning: PA5423 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3270 3.90e+03 AgCGTCaa.CATT
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3271 6.40e+02 TgtTgcgCct
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3730 4.30e+02 actgcCtaTTcgactA.Cc
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3731 3.40e+02 gGaAAaaC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA5423

PA5423 is enriched for 1 functions in 3 categories.
Enrichment Table (1)
Function System
Uncharacterized protein conserved in bacteria cog/ cog
Module neighborhood information for PA5423

PA5423 has total of 50 gene neighbors in modules 221, 457
Gene neighbors (50)
Gene Common Name Description Module membership
PA0062 PA0062 hypothetical protein (NCBI) 85, 221
PA0103 PA0103 probable sulfate transporter (NCBI) 85, 221
PA0309 PA0309 hypothetical protein (NCBI) 221, 551
PA0469 PA0469 hypothetical protein (NCBI) 376, 457
PA0566 PA0566 hypothetical protein (NCBI) 209, 457
PA0601 PA0601 probable two-component response regulator (NCBI) 53, 457
PA0810 PA0810 probable haloacid dehalogenase (NCBI) 185, 457
PA1036 PA1036 hypothetical protein (NCBI) 34, 457
PA1558 PA1558 hypothetical protein (NCBI) 220, 457
PA1603 PA1603 probable transcriptional regulator (NCBI) 53, 457
PA1607 PA1607 hypothetical protein (NCBI) 243, 457
PA1645 PA1645 hypothetical protein (NCBI) 229, 457
PA1889 PA1889 hypothetical protein (NCBI) 221, 547
PA1995 PA1995 hypothetical protein (NCBI) 457, 469
PA2145 PA2145 hypothetical protein (NCBI) 27, 221
PA2718 PA2718 probable transcriptional regulator (NCBI) 208, 457
PA2756 PA2756 hypothetical protein (NCBI) 63, 457
PA2790 PA2790 hypothetical protein (NCBI) 321, 457
PA2894 PA2894 hypothetical protein (NCBI) 27, 457
PA2899 PA2899 probable transcriptional regulator (NCBI) 208, 457
PA3069 PA3069 hypothetical protein (NCBI) 85, 221
PA3256 PA3256 probable oxidoreductase (NCBI) 405, 457
PA3259 PA3259 hypothetical protein (NCBI) 88, 457
PA3261 PA3261 hypothetical protein (NCBI) 248, 457
PA3354 PA3354 hypothetical protein (NCBI) 248, 457
PA3540 algD GDP-mannose 6-dehydrogenase AlgD (NCBI) 85, 221
PA3541 alg8 alginate biosynthesis protein Alg8 (NCBI) 85, 221
PA3542 alg44 alginate biosynthesis protein Alg44 (NCBI) 85, 221
PA3543 algK alginate biosynthetic protein AlgK precursor (NCBI) 85, 221
PA3544 algE Alginate production outer membrane protein AlgE precursor (NCBI) 85, 221
PA3545 algG alginate-c5-mannuronan-epimerase AlgG (NCBI) 85, 221
PA3546 algX alginate biosynthesis protein AlgX (NCBI) 85, 221
PA3547 algL poly(beta-D-mannuronate) lyase (NCBI) 85, 221
PA3548 algI alginate o-acetyltransferase AlgI (NCBI) 85, 221
PA3549 algJ alginate o-acetyltransferase AlgJ (NCBI) 85, 221
PA3550 algF alginate o-acetyltransferase AlgF (NCBI) 85, 221
PA3551 algA phosphomannose isomerase / guanosine 5'-diphospho-D-mannose pyrophosphorylase (NCBI) 85, 221
PA3576 PA3576 hypothetical protein (NCBI) 2, 457
PA3689 PA3689 probable transcriptional regulator (NCBI) 243, 457
PA3762 PA3762 hypothetical protein (NCBI) 221, 547
PA4111 PA4111 hypothetical protein (NCBI) 248, 457
PA4154 PA4154 hypothetical protein (NCBI) 85, 221
PA4338 PA4338 hypothetical protein (NCBI) 85, 221
PA4369 PA4369 hypothetical protein (NCBI) 452, 457
PA4955 PA4955 hypothetical protein (NCBI) 338, 457
PA5183 PA5183 hypothetical protein (NCBI) 85, 221
PA5258 PA5258 hypothetical protein (NCBI) 88, 457
PA5423 PA5423 hypothetical protein (NCBI) 221, 457
PA5483 algB two-component response regulator AlgB (NCBI) 107, 221
PA5484 PA5484 probable two-component sensor (NCBI) 107, 221
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA5423
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend